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Background: With the rapid growth of massively parallel sequencing technologies, still more laboratories are utilizing sequenced DNA fragments for genomic analyses. Interpretation of sequencing data is, however, strongly dependent on…

Reproducibility and sustainability present significant challenges in bioinformatics software development, where rapidly evolving tools and complex workflows often result in short-lived or difficult-to-adapt pipelines. This paper introduces…

软件工程 · 计算机科学 2025-05-07 Marco Masera , Alessandro Leone , Johannes Köster , Ivan Molineris

Summary: Mapping ancient DNA to a reference genome is challenging as it involves numerous steps, is time-consuming and has to be repeated within a study to assess the quality of extracts and libraries; as a result, the mapping needs to be…

Analyzing a functional genomics experiment, such as ATAC-, ChIP- or RNA-sequencing, requires reference data including a genome assembly and gene annotation. These resources can generally be retrieved from different organizations and in…

基因组学 · 定量生物学 2022-09-05 Siebren Frölich , Maarten van der Sande , Tilman Schäfers , Simon J. van Heeringen

We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and…

基因组学 · 定量生物学 2023-06-02 Phoebe C. R. Parrish , Daniel J. Groso , James D. Thomas , Robert K. Bradley , Alice H. Berger

Biosciences have been revolutionized by next generation sequencing (NGS) technologies in last years, leading to new perspectives in medical, industrial and environmental applications. And although our motivation comes from biosciences, the…

分布式、并行与集群计算 · 计算机科学 2017-01-16 Bruno Dantas , Calmenelias Fleitas , Alexandre P. Francisco , José Simão , Cátia Vaz

Modern DNA sequencing technologies enable geneticists to rapidly identify genetic variation among many human genomes. However, isolating the minority of variants underlying disease remains an important, yet formidable challenge for medical…

基因组学 · 定量生物学 2015-06-15 Uma Paila , Brad Chapman , Rory Kirchner , Aaron Quinlan

The emergence of R, a freely available data analysis environment, brought to the researcher in any science field a set of well-concerted instruments of immense power and low cost. In botany and zoology, these instruments could be used, for…

定量方法 · 定量生物学 2020-02-06 Alexey Shipunov

We present the first public release of ShapePipe, an open-source and modular weak-lensing measurement, analysis, and validation pipeline written in Python. We describe the design of the software and justify the choices made. We provide a…

天体物理仪器与方法 · 物理学 2022-08-24 S. Farrens , A. Guinot , M. Kilbinger , T. Liaudat , L. Baumont , X. Jimenez , A. Peel , A. Pujol , M. Schmitz , J. -L. Starck , A. Z. Vitorelli

Motivation: Quality control of genomic data is an essential but complicated multi-step procedure, often requiring separate installation and expert familiarity with a combination of disparate bioinformatics tools. Results: To provide an…

基因组学 · 定量生物学 2021-05-06 Christina Vasilopoulou , Benjamin Wingfield , Andrew P. Morris , William Duddy

High throughput genome sequencing technologies such as RNA-Seq and Microarray have the potential to transform clinical decision making and biomedical research by enabling high-throughput measurements of the genome at a granular level.…

Scaling up deep neural network capacity has been known as an effective approach to improving model quality for several different machine learning tasks. In many cases, increasing model capacity beyond the memory limit of a single…

计算机视觉与模式识别 · 计算机科学 2019-07-29 Yanping Huang , Youlong Cheng , Ankur Bapna , Orhan Firat , Mia Xu Chen , Dehao Chen , HyoukJoong Lee , Jiquan Ngiam , Quoc V. Le , Yonghui Wu , Zhifeng Chen

With the advent of Next-Generation (NG) sequencing, it has become possible to sequence an entire genome quickly and inexpensively. However, in some experiments one only needs to extract and assembly a portion of the sequence reads, for…

基因组学 · 定量生物学 2013-08-01 Ilya Y. Zhbannikov , Samuel S. Hunter , Matthew L. Settles , James A. Foster

Variant calling is a fundamental task in genomic research, essential for detecting genetic variations such as single nucleotide polymorphisms (SNPs) and insertions or deletions (indels). This paper presents an enhancement to DeepChem, a…

定量方法 · 定量生物学 2025-07-29 Ankita Vaishnobi Bisoi , Shreyas V , Jose Siguenza , Bharath Ramsundar

Non-sharable sensitive data collection and analysis in large-scale consortia for genomic research is complicated. Time consuming issues in installing software arise due to different operating systems, software dependencies and running the…

PaPy, which stands for parallel pipelines in Python, is a highly flexible framework that enables the construction of robust, scalable workflows for either generating or processing voluminous datasets. A workflow is created from user-written…

编程语言 · 计算机科学 2014-07-17 Marcin Cieslik , Cameron Mura

Scalable and efficient processing of genome sequence data, i.e. for variant discovery, is key to the mainstream adoption of High Throughput technology for disease prevention and for clinical use. Achieving scalability, however, requires a…

分布式、并行与集群计算 · 计算机科学 2018-06-05 Nicholas Tucci , Jacek Cala , Jannetta Steyn , Paolo Missier

The marine environment is one of the most important sources for microbial biodiversity on the planet. These microbes are drivers for many biogeochemical processes, and their enormous genetic potential is still not fully explored or…

Advances in genome sequencing technologies generate massive amounts of sequence data that are increasingly analyzed and shared through public repositories. On-demand infrastructure services on cloud computing platforms enable the processing…

分布式、并行与集群计算 · 计算机科学 2025-03-20 Junseok Park , Eduardo A. Maury , Changhoon Oh , Donghoon Shin , Danielle Denisko , Eunjung Alice Lee

Compendium Manager is a command-line tool written in Python to automate the provisioning, launch, and evaluation of bioinformatics pipelines. Although workflow management tools such as Snakemake and Nextflow enable users to automate the…

基因组学 · 定量生物学 2025-05-19 Richard J. Abdill , Ran Blekhman
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