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The spectroscopic response of and structural dynamics around all azido-modified alanine residues (AlaN$_3$) in Lysozyme is characterized. It is found that AlaN$_3$ is a positionally sensitive probe for the local dynamics, covering a…

生物物理 · 物理学 2024-06-19 Seyedeh Maryam Salehi , Markus Meuwly

The changes in the local and global dynamics of azide-labelled Lysozyme compared with that of the wild type protein are quantitatively assessed for all alanine residues along the polypeptide chain. Although attaching -N$_3$ to alanine…

化学物理 · 物理学 2021-12-02 Seyedeh Maryam Salehi , Markus Meuwly

The dynamics of lysozyme is probed by attaching -SCN to all alanine-residues. The 1-dimensional infrared spectra exhibit frequency shifts in the position of the maximum absorption by 4 cm$^{-1}$ which is consistent with experiments in…

化学物理 · 物理学 2024-04-30 Sena Aydin , Seyedeh Maryam Salehi , Kai Töpfer , Markus Meuwly

While allostery is of paramount importance for protein regulation, the underlying dynamical process of ligand (un)binding at one site, resulting time evolution of the protein structure, and change of the binding affinity at a remote site is…

Prediction of protein-ligand binding affinity is a major goal in drug discovery. Generally, free energy gap is calculated between two states (e.g., ligand binding and unbinding). The energy gap implicitly includes the effects of changes in…

生物大分子 · 定量生物学 2022-05-20 Ikki Yasuda , Katsuhiro Endo , Eiji Yamamoto , Yoshinori Hirano , Kenji Yasuoka

In this work we present a detailed Raman scattering investigation of zinc oxide and aluminum-doped zinc oxide (AZO) films characterized by a variety of nanoscale structure and morphology and synthesized by pulsed laser deposition (PLD)…

材料科学 · 物理学 2017-10-12 V. Russo , M. Ghidelli , P. Gondoni , C. S. Casari , A. Li Bassi

Isotope labeling is a fundamental staple for the study of cellular metabolism and protein function. The conventional techniques that allow resolution and identification of isotopically-labeled biomarkers, such as mass spectrometry and…

The structural, electronic, optical and vibrational properties of LiN$_3$ under high pressure have been studied using plane wave pseudopotentials within the generalized gradient approximation for the exchange and correlation functional. The…

材料科学 · 物理学 2013-11-07 K. Ramesh Babu , Ch. Bheema Lingam , Surya P. Tewari , G. Vaitheeswaran

We analyze free energy estimators from simulation trials mimicking single-molecule pulling experiments on a neck linker of a kinesin motor. For that purpose, we have performed a version of steered molecular dynamics (SMD) calculations. The…

生物大分子 · 定量生物学 2018-09-19 Michał Świątek , Ewa Gudowska-Nowak

Determining the complete set of ligands' binding/unbinding pathways is important for drug discovery and to rationally interpret mutation data. Here we have developed a metadynamics-based technique that addressed this issue and allows…

化学物理 · 物理学 2019-04-25 Riccardo Capelli , Paolo Carloni , Michele Parrinello

We report density functional theory calculations on the crystal structure, elastic, lattice dynamics and electronic properties of iso-structural layered monoclinic alkali azides, LiN3 and NaN3. The effect of van der Waals interactions on…

材料科学 · 物理学 2013-11-04 K. Ramesh Babu , G. Vaitheeswaran

While much is known about different allosteric regulation mechanisms, the nature of the "allosteric signal", and the timescale on which it propagates, remains elusive. The PDZ3 domain from postsynaptic density-95 protein is a small protein…

Monitoring the kinetics and conformational dynamics of single enzymes is crucial in order to better understand their biological functions as these motions and structural dynamics are usually unsynchronized among the molecules. Detecting the…

生物物理 · 物理学 2017-05-29 Eugene Kim , Martin D. Baaske , Isabel Schuldes , Peter S. Wilsch , Frank Vollmer

Active Domain Adaptation (ADA) queries the labels of a small number of selected target samples to help adapting a model from a source domain to a target domain. The local context of queried data is important, especially when the domain gap…

机器学习 · 计算机科学 2023-08-29 Tao Sun , Cheng Lu , Haibin Ling

The protein dynamical transition is investigated as a function of protein structure using terahertz time domain spectroscopy (THz-TDS). Measurements performed for native state and denatured hen egg white lysozyme (HEWL) show that protein…

生物物理 · 物理学 2008-07-23 Yunfen He , Andrea G. Markelz

The electronic structure and the anisotropy of the Al - N {\pi} and {\sigma} chemical bonding of wurtzite AlN has been investigated by bulk-sensitive total fluorescence yield absorption and soft x-ray emission spectroscopies. The measured N…

材料科学 · 物理学 2011-12-30 M. Magnuson , M. Mattesini , C. Höglund , J. Birch , L. Hultman

Prediction of ligand binding sites of proteins is a fundamental and important task for understanding the function of proteins and screening potential drugs. Most existing methods require experimentally determined protein holo-structures as…

定量方法 · 定量生物学 2023-12-07 Shuo Zhang , Lei Xie

Protein-ligand complex structures have been utilised to design benchmark machine learning methods that perform important tasks related to drug design such as receptor binding site detection, small molecule docking and binding affinity…

生物大分子 · 定量生物学 2021-08-26 Rishal Aggarwal , Akash Gupta , U Deva Priyakumar

Allosteric interactions in DNA are crucial for various biological processes. These interactions are quantified by measuring the change in free energy as a function of the distance between the binding sites for two ligands. Here we show that…

生物物理 · 物理学 2018-05-15 Jaspreet Singh , Prashant K. Purohit

The influence of ligands on the low frequency vibration of different thicknesses cadmium selenide colloidal nanoplatelets is investigated using resonant low frequency Raman scattering. The strong vibration frequency shifts induced by ligand…

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