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Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs),…

基因组学 · 定量生物学 2015-11-24 Nikolai Slavov , Sefan Semrau , Edoardo Airoldi , Bogdan Budnik , Alexander van Oudenaarden

The ribosomal density along the coding region of the mRNA molecule affect various fundamental intracellular phenomena including: protein production rates, organismal fitness, ribosomal drop off, and co-translational protein folding. Thus,…

基因组学 · 定量生物学 2017-05-19 Yoram Zarai , Michael Margaliot , Eduardo D. Sontag , Tamir Tuller

The process of polymerizing a protein by a ribosome, using a messenger RNA (mRNA) as the corresponding template, is called {\it translation}. Ribosome may be regarded as a molecular motor for which the mRNA template serves also as the…

生物物理 · 物理学 2015-03-19 Ajeet K. Sharma , Debashish Chowdhury

Many pressing medical challenges - such as diagnosing disease, enhancing directed stem cell differentiation, and classifying cancers - have long been hindered by limitations in our ability to quantify proteins in single cells.…

基因组学 · 定量生物学 2018-08-06 Harrison Specht , Nikolai Slavov

Translation is an important process for prokaryotic and eukaryotic cells to produce necessary proteins for cell growth. Numerious experiments have been performed to explore the translational properties. Diverse models have also been…

生物物理 · 物理学 2015-02-10 Jingwei Li , Yunxin Zhang

Research in the life sciences often employs messenger ribonucleic acids (mRNA) quantification as a standalone approach for functional analysis. However, although the correlation between the measured levels of mRNA and proteins is positive,…

定量方法 · 定量生物学 2025-01-07 Romain-Daniel Gosselin

Gene expression consists in the synthesis of proteins from the information encoded on DNA. One of the two main steps of gene expression is the translation of messenger RNA (mRNA) into polypeptide sequences of amino acids. Here, by taking…

Using the perturbation-response scanning (PRS) technique, we study a set of 23 proteins that display a variety of conformational motions upon ligand binding (e.g. shear, hinge, allosteric). In most cases, PRS determines residues that may be…

生物大分子 · 定量生物学 2015-05-18 C Atilgan , Z N Gerek , S B Ozkan , A R Atilgan

Down regulation of mRNA translation is an important problem in various bio-medical domains ranging from developing effective medicines for tumors and for viral diseases to developing attenuated virus strains that can be used for…

基因组学 · 定量生物学 2016-06-29 Yoram Zarai , Michael Margaliot , Tamir Tuller

In covariate-adaptive or response-adaptive randomization, the treatment assignment and outcome can be correlated. Under this situation, re-randomization tests are a straightforward and attractive method to provide valid statistical…

统计方法学 · 统计学 2023-03-14 Yilong Zhang , Yujie Zhao , Yiwen Luo

The increasing complexity of medical imaging data underscores the need for advanced anomaly detection methods to automatically identify diverse pathologies. Current methods face challenges in capturing the broad spectrum of anomalies, often…

图像与视频处理 · 电气工程与系统科学 2024-01-22 Cosmin I. Bercea , Benedikt Wiestler , Daniel Rueckert , Julia A. Schnabel

Transcriptional and post-transcriptional regulation shape tissue-type-specific proteomes, but their relative contributions remain contested. Estimates of the factors determining protein levels in human tissues do not distinguish between (i)…

基因组学 · 定量生物学 2017-05-15 Alexander Franks , Edoardo Airoldi , Nikolai Slavov

The variation in DNA copy number carries information on the modalities of genome evolution and misregulation of DNA replication in cancer cells; its study can be helpful to localize tumor suppressor genes, distinguish different populations…

统计方法学 · 统计学 2012-03-20 Zhongyang Zhang , Kenneth Lange , Chiara Sabatti

Many {\it ribosomes} simultaneously move on the same messenger RNA (mRNA), each synthesizing separately a copy of the same protein. In contrast to the earlier models, here {\it we develop a ``unified'' theoretical model} that not only…

生物物理 · 物理学 2007-05-23 Aakash Basu , Debashish Chowdhury

We develop a stochastic model for the programmed frameshift of ribosomes synthesizing a protein while moving along a mRNA template. Normally the reading frame of a ribosome decodes successive triplets of nucleotides on the mRNA in a…

生物物理 · 物理学 2017-06-01 Bhavya Mishra , Gunter M. Schütz , Debashish Chowdhury

Proteins are polymerized by cyclic machines called ribosome which use their messenger RNA (mRNA) track also as the corresponding template and the process is called translation. We explore, in depth and detail, the stochastic nature of the…

生物物理 · 物理学 2009-11-13 Ashok Garai , Debashish Chowdhury , T. V. Ramakrishnan

Radiomics analysis has emerged as a promising approach for extracting quantitative features from medical images to aid in cancer diagnosis and treatment. However, radiomics research currently lacks standardization, and radiomics features…

Intrinsically disordered proteins and regions are increasingly appreciated for their abundance in the proteome and the many functional roles they play in the cell. In this short review, we describe a variety of approaches used to obtain…

生物物理 · 物理学 2024-12-31 Zi Hao Liu , Maria Tsanai , Oufan Zhang , Teresa Head-Gordon , Julie Forman-Kay

Translation of proteins is a fundamental part of gene expression that is mediated by ribosomes. As ribosomes significantly contribute to both cellular mass and energy consumption, achieving efficient management of the ribosome population is…

亚细胞过程 · 定量生物学 2024-04-18 Clément Soubrier , Eric Foxall , Luca Ciandrini , Khanh Dao Duc

Replicability and reproducibility of experimental results are primary concerns in all the areas of science and IR is not an exception. Besides the problem of moving the field towards more reproducible experimental practices and protocols,…

信息检索 · 计算机科学 2020-10-27 Timo Breuer , Nicola Ferro , Norbert Fuhr , Maria Maistro , Tetsuya Sakai , Philipp Schaer , Ian Soboroff
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