相关论文: Developing a new biophysical tool to combine magne…
Ultrafast ultrasound localization microscopy (ULM), which has shown promising results in microvascular imaging, overcomes the typical trade-off between resolution and penetration depth. Combining ultrasound contrast agents and high frame…
Time-lapse fluorescence microscopy (TLFM) is an important and powerful tool in synthetic biological research. Modeling TLFM experiments based on real data may enable researchers to repeat certain experiments with minor effort. This thesis…
Identification of every single genome present in a microbial sample is an important and challenging task with crucial applications. It is challenging because there are typically millions of cells in a microbial sample, the vast majority of…
Soft actuators that respond to external stimuli play a fundamental role in microscale robotics, active matter, and bio-inspired systems. Among these actuators, photo-thermal hybrid microgels (HMGs) containing plasmonic nanoparticles enable…
Holographic optical tweezers can be applied to manipulate microscopic particles in arbitrary optical patterns, which classical optical tweezers cannot do. This ability relies on accurate computer-generated holography (CGH), yet most CGH…
We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…
The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. This manuscript reports the development of Polarized Localization Microscopy (PLM),…
We propose and demonstrate a novel technique that combines Raman scattering and optical cycling in molecules with diagonal Franck-Condon factors. This resonance Raman optical cycling manipulates molecules to behave like efficient…
Simultaneous, diffraction-limited control of multiple optical beams is crucial for applications ranging from lithography to optogenetics, deep tissue imaging, and tweezer-based manipulation of cells, particles, or atoms. Despite the desire…
We present an experimental procedure to perform broadband microrheological measurements with optical tweezers. A generalised Langevin equation is adopted to relate the time-dependent trajectory of a particle in an imposed flow to the…
This paper presents an experimental investigation on pattern formation and evolution in bilayered tubular organs using swelling deformation of polydimethylsiloxane (PDMS) and aims at supplying a thorough comparison with theoretical and…
Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…
Traction Force Microscopy (TFM) is a technique used to determine the tensions that a biological cell conveys to the underlying surface. Typically, TFM requires culturing cells on gels with fluorescent beads, followed by bead displacement…
Plasmonic nanopores hold a significant promise for molecular sequencing, but their sensitivity and temporal resolution are constrained by limited signal strength and rapid translocation of molecules through the pore. Here we report an…
The spin-orbit interaction (SOI) of light generated by tight focusing in optical tweezers has been regularly employed in generating angular momentum - both spin and orbital - in trapped mesoscopic particles. Specifically, the transverse…
Magnetic imaging with ultra-high spatial resolution is crucial to exploring the magnetic textures of emerging quantum materials. We propose a novel magnetic imaging protocol that achieves Angstrom-scale resolution by combining spin defects…
Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…
Mode division multiplexing (MDM) is mooted as a technology to address future bandwidth issues, and has been successfully demonstrated in free space using spatial modes with orbital angular momentum (OAM). To further increase the data…
Optical tweezers, the three-dimensional confinement of a nanoparticle by a strongly focused beam of light, have been widely employed in investigating biomaterial nanomechanics, nanoscopic fluid properties, and ultrasensitive detections in…
Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…