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Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

Optical tweezers are highly versatile laser traps for neutral microparticles, with fundamental applications in physics and in single molecule cell biology. Force measurements are performed by converting the stiffness response to…

光学 · 物理学 2014-06-30 R S Dutra , N B Viana , P A Maia Neto , H M Nussenzveig

We propose an enantioselective scheme to sort homogeneous chiral particles using optical tweezers. For a certain range of material parameters, we show that a highly focused circularly-polarized laser beam traps particles of a specific…

光学 · 物理学 2023-07-19 R. Ali , F. A. Pinheiro , R. S. Dutra , F. S. S. Rosa , P. A. Maia Neto

Droplet microlasers, as promising tools for biophotonics and biomedical sciences, have witnessed rapid advances due to their flexible reconfigurability, high sensitivity to stimuli, and label-free biosensing ability. However, designing…

We present the first successful trapping of single erbium atoms in an array of optical tweezers. Using a single narrow-line optical transition, we achieve deep cooling for direct tweezer loading, pairwise ejection, and continous imaging…

原子物理 · 物理学 2025-04-04 D. S. Grün , S. J. M. White , A. Ortu , A. Di Carli , H. Edri , M. Lepers , M. J. Mark , F. Ferlaino

Biophotonic techniques are growing in rapid rhythms enabling the monitoring of subcellular structures and non-invasive theranostic interventions in cancer and autoimmune diseases. The integration of Biophotonics with nanotechnology and…

医学物理 · 物理学 2021-06-15 Ellas Spyratou

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

光学 · 物理学 2021-02-02 Oumeng Zhang , Matthew D. Lew

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

The manipulation of nanoscale matter has the potential to revolutionize a variety of fields across nanoscience and technology. Here, we demonstrate experimentally and characterize numerically a device that combines the benefits of…

介观与纳米尺度物理 · 物理学 2024-08-13 Marco Riccardi , Olivier J. F. Martin

Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

图像与视频处理 · 电气工程与系统科学 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

Current optical manipulation techniques rely on carefully engineered setups and samples. Although similar conditions are routinely met in research laboratories, it is still a challenge to manipulate microparticles when the environment is…

光学 · 物理学 2014-07-22 Giorgio Volpe , Lisa Kurz , Agnese Callegari , Giovanni Volpe , Sylvain Gigan

Holographic Optical Tweezers (HOT) are powerful tools that can manipulate micro and nano-scale objects with high accuracy and precision. They are most commonly used for biological applications, such as cellular studies, and more recently,…

系统与控制 · 电气工程与系统科学 2024-04-29 Tommy Zhang , Nicole Werner , Ashis G. Banerjee

Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…

The movement of molecules inside living cells is a fundamental feature of biological processes. The ability to both observe and analyse the details of molecular diffusion in vivo at the single molecule and single cell level can add…

定量方法 · 定量生物学 2012-11-21 Alex Robson , Kevin Burrage , Mark Leake

Optical tweezers (OT) have become an essential technique in several fields of physics, chemistry, and biology as precise micromanipulation tools and microscopic force transducers. Quantitative measurements require the accurate calibration…

The ability to create dynamic, tailored optical potentials has become important across fields ranging from biology to quantum science. We demonstrate a method for the creation of arbitrary optical tweezer potentials using the broadband…

光学 · 物理学 2020-07-16 A. T. M. Anishur Rahman , P. F. Barker

Non-invasive optical manipulation of particles has emerged as a powerful and versatile tool for biological study and nanotechnology. In particular, trapping and rotation of cells, cell nuclei and sub-micron particles enables unique…

There is a trend in research towards more automation using smart systems powered by artificial intelligence. While experiments are often challenging to automate, they can greatly benefit from automation by reducing labor and increasing…