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To be able to resolve molecular-clusters it is crucial to access vital informations (such as, molecule density and cluster-size) that are key to understand disease progression and the underlying mechanism. Traditional single-molecule…

定量方法 · 定量生物学 2021-01-27 Partha Pratim Mondal

Coherently controlling the motion of single atoms in optical tweezers would enable new applications in quantum information science. To demonstrate this, we first prepare atoms in their motional ground state using a species-agnostic cooling…

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Efficient capture and detection of minute amount of deoxyribonucleic acid (DNA) molecules are pivotal for an array of modern gene technologies which are widely utilized in medical, forensic and defense applications, including DNA…

生物物理 · 物理学 2018-11-19 Jianhe Guo , Kwanoh Kim , Peter Vandeventer , Donglei , Fan

Acoustic tweezers can manipulate microscopic objects and cells independently of the optical, magnetic and electrical properties of the objects or their medium. However, because ultrasonic waves are attenuated within few millimeters,…

流体动力学 · 物理学 2022-03-29 Qing Wang , Shuhan Chen , Jia Zhou , Antoine Riaud

We experimentally demonstrate stable trapping and controlled manipulation of silica microspheres in a structured optical beam consisting of a dark focus surrounded by light in all directions - the so-called Dark Focus Tweezer. Results from…

All light has structure, but only recently it has become possible to construct highly controllable and precise potentials so that most laboratories can harness light for their specific applications. In this chapter, we review the emerging…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

图像与视频处理 · 电气工程与系统科学 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Ultrasound localization microscopy has enabled super-resolution vascular imaging through precise localization of individual ultrasound contrast agents (microbubbles) across numerous imaging frames. However, analysis of high-density regions…

信号处理 · 电气工程与系统科学 2018-12-14 Ruud J. G. van Sloun , Oren Solomon , Matthew Bruce , Zin Z. Khaing , Hessel Wijkstra , Yonina C. Eldar , Massimo Mischi

We have constructed a counterpropagating optical tweezers setup embedded in a Sagnac interferometer in order to increase the sensitivity of position tracking for particles in the geometrical optics regime. Enhanced position determination…

Optical tweezers are powerful tools based on focused laser beams. They are able to trap, manipulate and investigate a wide range of microscopic and nanoscopic particles in different media, such as liquids, air, and vacuum. Key applications…

We explain and demonstrate a new method of force- and position-calibration for optical tweezers with back-focal-plane photo detection. The method combines power spectral measurements of thermal motion and the response to a sinusoidal motion…

A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

图像与视频处理 · 电气工程与系统科学 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman

Optically levitated dielectric nanoparticles have become valuable tools for precision sensing and quantum optomechanical experiments. To predict the dynamic properties of a particle trapped in an optical tweezer with high fidelity, a tool…

光学 · 物理学 2024-11-20 Moosung Lee , Tobias Hanke , Sara Launer , Sungkun Hong

Acoustical tweezers open major prospects in microbiology for cells and microorganisms contactless manipulation, organization and mechanical properties testing since they are biocompatible, label-free and can exert forces several orders of…

Optical tweezers employing forces produced by light underpin important manipulation tools in many areas of applied and biological physics. Conventional optical tweezers are based on refractive optics, and they require excessive auxiliary…

Conventional optical tweezers are generated by the intensity gradient of highly focused laser beams, but the requirement of strong intensity gradient limits the tunability of optical traps. Here we show a new type of optical tweezers with…

光学 · 物理学 2019-12-03 Xionggui Tang , Fan Nan , Zijie Yan

We have developed a versatile near-field microscopy platform that can operate at high magnetic fields and below liquid-helium temperatures. We use this platform to demonstrate an extreme terahertz (THz) nanoscope operation and to obtain the…

介观与纳米尺度物理 · 物理学 2023-08-17 Richard H. J. Kim , Joong-Mok Park , Samuel J. Haeuser , Liang Luo , Jigang Wang

The increasing importance of artificial intelligence and machine learning in materials research has created demand for automated, high-throughput characterization techniques capable of rapidly generating large data sets. We describe here a…

Diffuse optical imaging (DOI) offers valuable insights into scattering mediums, but the quest for high-resolution imaging often requires dense sampling strategies, leading to higher imaging errors and lengthy acquisition times. This work…

光学 · 物理学 2025-04-07 Ben Wiesel , Shlomi Arnon
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