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Microrheology is a branch of rheology having the same principles as conventional bulk rheology, but working on micron length scales and micro-litre volumes. Optical tweezers have been successfully used with Newtonian fluids for rheological…

Optical tweezers enable non-contact trapping of micro-scale objects using light. Despite their widespread use, it is currently not known how tightly it is possible to three-dimensionally trap micro-particles with a given photon budget.…

Sub-micrometer scale light patterns play a pivotal role in various fields, including biology, biophysics, and AMO physics. High-resolution, in situ observation of light profiles is essential for their design and application. However,…

We propose effective generation of entangled and squeezed states in an optoelectromechanical system comprising of a macroscopic LC electrical circuit and an optomechanical system. We obtain enhanced entanglement between optical and LC…

量子物理 · 物理学 2022-10-19 Sampreet Kalita , Saumya Shah , Amarendra K. Sarma

A non-invasive, in-situ calibration method for Total Internal Reflection Microscopy (TIRM) based on optical tweezing is presented which greatly expands the capabilities of this technique. We show that by making only simple modifications to…

光学 · 物理学 2016-05-27 Lulu Liu , Alexander Woolf , Alejandro W. Rodriguez , Federico Capasso

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

Lightwave-driven scanning tunnelling microscopy (STM) at near-IR frequencies promises an unprecedented combination of atomic spatial resolution and temporal resolution approaching the attosecond range. To achieve this goal, high-sensitivity…

Microfabrication using nano- to micron-sized building blocks holds great potential for applications in next-generation electronics, optoelectronics, and advanced materials. However, traditional methods like chemical vapor deposition and…

Trapped atoms near nanophotonics form an exciting platform for bottom-up synthesis of strongly interacting quantum matter. The ability to induce tunable long-range atom-atom interactions with photons presents an opportunity to explore…

量子物理 · 物理学 2019-04-12 May E. Kim , Tzu-Han Chang , Brian M. Fields , Cheng-An Chen , Chen-Lung Hung

Polyatomic molecules have rich structural features that make them uniquely suited to applications in quantum information science, quantum simulation, ultracold chemistry, and searches for physics beyond the Standard Model. However, a key…

Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…

仪器与探测器 · 物理学 2018-10-12 Matthias Eibl , Sebastian Karpf , Hubertus Hakert , Daniel Weng , Robert Huber

Oscillatory dynamics is a key tool in optical tweezers applications. It is usually implemented by mechanical interventions that cannot be optically controlled. In this work we show that Germanium semiconductor beads behave as optically…

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

细胞行为 · 定量生物学 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

Optical tweezers have become essential tools to manipulate atoms or molecules at a single particle level. However, using standard diffracted-limited optical systems, the transverse size of the trap is lower bounded by the optical…

We demonstrate a method to double the collection efficiency in Laser Tweezers Raman Spectroscopy (LTRS) by collecting both the forward and back-scattered light in a single-shot multitrack measurement. Our method can collect signals at…

光学 · 物理学 2021-06-09 Tobias Dahlberg , Magnus Andersson

Single molecule localization microscopy (SMLM) permits the visualization of cellular structures an order of magnitude smaller than the diffraction limit of visible light, and an accurate, objective evaluation of the resolution of an SMLM…

Improving the detailed understanding of the underlying properties and functions of biomolecules has recently attracted growing interest, enabled by the possibility of real-space imaging of single, intact macromolecules using Scanning…

介观与纳米尺度物理 · 物理学 2026-03-10 Tim J. Seifert , Dhaneesh Kumar , Markus Etzkorn , Stephan Rauschenbach , Klaus Kern , Kelvin Anggara , Uta Schlickum

Dual trap optical tweezers are often used in high-resolution measurements in single-molecule biophysics. Such measurements can be hindered by the presence of extraneous noise sources, the most prominent of which is the coupling of…

生物物理 · 物理学 2013-07-03 Marco Ribezzi Crivellari , Felix Ritort

In this article we explore the requirements for enabling high quality optically detected magnetic resonance (ODMR) spectroscopy in a conventional gradient force optical tweezers using nanodiamonds containing nitrogen-vacancy (NV$^{-}$)…

光学 · 物理学 2021-01-25 L. W. Russell , E. C. Dossetor , A. A. Wood , D. A. Simpson , P. J. Reece

In this paper, we show how one can change the stable equilibrium of a particle trapped into an optical tweezer by varying the intensity of superposed Bessel beams with different orders. The gradient forces acting on particles of different…