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Modern developments in microscopy and image processing are revolutionizing areas of physics, chemistry and biology as nanoscale objects can be tracked with unprecedented accuracy. The goal of single particle tracking is to determine the…

统计力学 · 物理学 2015-06-04 Denis Boyer , David S. Dean , Carlos Mejía-Monasterio , Gleb Oshanin

Modern developments in microscopy and image processing are revolutionising areas of physics, chemistry, and biology as nanoscale objects can be tracked with unprecedented accuracy. However, the price paid for having a direct visualisation…

统计力学 · 物理学 2013-03-19 Denis Boyer , David S. Dean , Carlos Mejía-Monasterio , Gleb Oshanin

Time course measurement of single molecules on a cell surface provides detailed information on the dynamics of the molecules, which is otherwise inaccessible. To extract the quantitative information, single particle tracking (SPT) is…

定量方法 · 定量生物学 2016-01-06 Shunsuke Teraguchi , Yutaro Kumagai

We discuss the distribution of various estimators for extracting the diffusion constant of single Brownian trajectories obtained by fitting the squared displacement of the trajectory. The analysis of the problem can be framed in terms of…

统计力学 · 物理学 2015-05-28 Denis Boyer , David S. Dean

The movement of a particle described by Brownian motion is quantified by a single parameter, $D$, the diffusion constant. The estimation of $D$ from a discrete sequence of noisy observations is a fundamental problem in biological single…

亚细胞过程 · 定量生物学 2016-04-13 Peter K. Relich , Mark J. Olah , Patrick J. Cutler , Keith A. Lidke

Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…

生物大分子 · 定量生物学 2011-07-12 Shannon Kian Zareh , Michael C. DeSantis , Jonathan Kessler , Je-Luen Li , Y. M. Wang

Single-particle tracking (SPT) has become a popular tool to study the intracellular transport of molecules in living cells. Inferring the character of their dynamics is important, because it determines the organization and functions of the…

定量方法 · 定量生物学 2020-09-09 Joanna Janczura , Patrycja Kowalek , Hanna Loch-Olszewska , Janusz Szwabiński , Aleksander Weron

Single-particle tracking allows to infer the motion of single molecules in living cells. When we observe a long trajectory (more than 100 points), it is possible that the particle switches mode of motion over time. Then, fitting a single…

统计方法学 · 统计学 2018-04-16 Vincent Briane , Charles Kervrann , Myriam Vimond

A standard approach to study time-dependent stochastic processes is the power spectral density (PSD), an ensemble-averaged property defined as the Fourier transform of the autocorrelation function of the process in the asymptotic limit of…

统计力学 · 物理学 2019-09-04 Vittoria Sposini , Ralf Metzler , Gleb Oshanin

An increasing number of experimental studies employ single particle tracking to probe the physical environment in complex systems. We here propose and discuss new methods to analyze the time series of the particle traces, in particular, for…

A Langevin process diffusing in a periodic potential landscape has a time dependent diffusion constant which means that its average mean squared displacement (MSD) only becomes linear at late times. The long time, or effective diffusion…

统计力学 · 物理学 2015-06-19 David S. Dean , Gleb Oshanin

Single-molecule localization microscopy allows practitioners to locate and track labeled molecules in biological systems. When extracting diffusion coefficients from the resulting trajectories, it is common practice to perform a linear fit…

生物物理 · 物理学 2024-06-19 Jakob Tómas Bullerjahn , Gerhard Hummer

We study efficiency of non-parametric estimation of diffusions (stochastic differential equations driven by Brownian motion) from long stationary trajectories. First, we introduce estimators based on conditional expectation which is…

概率论 · 数学 2021-05-26 Xi Chen , Ilya Timofeyev

In molecular dynamics simulations in the NPT ensemble at constant pressure, the size and shape of the periodic simulation box fluctuate with time. For particle images far from the origin, the rescaling of the box by the barostat results in…

The fundamental insight into Brownian motion by Einstein is that all substances exhibit continual fluctuations due to thermal agitation balancing with the frictional resistance. However, even at thermal equilibrium, biological activity can…

软凝聚态物质 · 物理学 2014-02-19 Soya Shinkai , Yuichi Togashi

Single Particle Tracking (SPT) can aid in understanding complex spatio-temporal processes. However, quantifying diffusivity and forces from individual live cell trajectories is complicated by inter- & intra-trajectory kinetic heterogeneity,…

定量方法 · 定量生物学 2016-05-19 Christopher P. Calderon

The exact analytical expressions for the time-dependent cross-correlations of the translational and rotational Brownian displacements of a particle with arbitrary shape were derived by us in [J. Chem. Phys. 142, 214902 (2015) and 144,…

软凝聚态物质 · 物理学 2018-10-09 B. Cichocki , M. L. Ekiel-Jezewska , E. Wajnryb

Single Particle Tracking (SPT) is a powerful class of tools for analyzing the dynamics of individual biological macromolecules moving inside living cells. The acquired data is typically in the form of a sequence of camera images that are…

生物大分子 · 定量生物学 2020-01-23 Ye Lin , Sean B. Andersson

Heterogeneous diffusion processes occur in many different fields such as transport in living cells or diffusion in porous media. A characterization of the transport parameters of such processes can be achieved by ensemble-based methods,…

数据分析、统计与概率 · 物理学 2011-10-17 Michael Bauer , Rustem Valiullin , Günter Radons , Jörg Kärger

We study the first-passage-time (FPT) properties of active Brownian particles to reach an absorbing wall in two dimensions. Employing a perturbation approach we obtain exact analytical predictions for the survival and FPT distributions for…

软凝聚态物质 · 物理学 2025-03-10 Yanis Baouche , Magali Le Goff , Christina Kurzthaler , Thomas Franosch
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