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The kinetic folding of RNA sequences into secondary structures is modeled as a complex adaptive system, the components of which are possible RNA structural rearrangements (SRs) and their associated bases and base pairs. RNA bases and base…

生物大分子 · 定量生物学 2007-05-23 Wilfred Ndifon

In biology, predicting RNA secondary structures plays a vital role in determining its physical and chemical properties. Although we have powerful energy models to predict them as well as parametric analysis to understand the models…

生物大分子 · 定量生物学 2023-05-01 Doan Dai Nguyen

RNA function is intimately related to its structural dynamics. Molecular dynamics simulations are useful for exploring biomolecular flexibility but are severely limited by the accessible timescale. Enhanced sampling methods allow this…

生物大分子 · 定量生物学 2018-02-06 Vojtěch Mlýnský , Giovanni Bussi

RNA function crucially depends on its structure. Thermodynamic models currently used for secondary structure prediction rely on computing the partition function of folding ensembles, and can thus estimate minimum free-energy structures and…

生物大分子 · 定量生物学 2022-07-26 Nicola Calonaci , Alisha Jones , Francesca Cuturello , Michael Sattler , Giovanni Bussi

Atomically detailed simulations of RNA folding have proven very challenging in view of the difficulties of developing realistic force fields and the intrinsic computational complexity of sampling rare conformational transitions. To tackle…

软凝聚态物质 · 物理学 2022-05-26 Gianmarco Lazzeri , Cristian Micheletti , Samuela Pasquali , Pietro Faccioli

We analyze a microscopic RNA model, which includes two widely used models as limiting cases, namely it contains terms for bond as well as for stacking energies. We numerically investigate possible changes in the qualitative and quantitative…

定量方法 · 定量生物学 2009-11-10 Bernd Burghardt , Alexander K. Hartmann

Although RNAs play many cellular functions little is known about the dynamics and thermodynamics of these molecules. In principle, all-atom molecular dynamics simulations can investigate these issues, but with current computer facilities,…

生物大分子 · 定量生物学 2013-01-22 Tristan Cragnolini , Philippe Derreumaux , Samuela Pasquali

RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by…

生物物理 · 物理学 2015-06-26 S. Harlepp , T. Marchal , J. Robert , J-F. Leger , A. Xayaphoummine , H. Isambert , D. Chatenay

The statistical mechanics of heteropolymer structure formation is studied in the context of RNA secondary structures. A designed RNA sequence biased energetically towards a particular native structure (a hairpin) is used to study the…

统计力学 · 物理学 2009-10-31 R. Bundschuh , T. Hwa

Recent computational efforts have shown that the current potential energy models used in molecular dynamics are not accurate enough to describe the conformational ensemble of RNA oligomers and suggest that molecular dynamics should be…

计算物理 · 物理学 2016-12-23 Andrea Cesari , Alejandro Gil-Ley , Giovanni Bussi

The process of RNA base fraying (i.e. the transient opening of the termini of a helix) is involved in many aspects of RNA dynamics. We here use molecular dynamics simulations and Markov state models to characterize the kinetics of RNA…

计算物理 · 物理学 2019-04-22 Giovanni Pinamonti , Fabian Paul , Frank Noé , Alex Rodriguez , Giovanni Bussi

In a previous paper we have introduced a phenomenological model of cell metabolism and of the cell cycle to simulate the behavior of large tumor cell populations (Chignola R and Milotti E, Phys. Biol. 2 (2005) 8-22). Here we describe a…

生物物理 · 物理学 2009-11-13 Roberto Chignola , Alessio Del Fabbro , Chiara Dalla Pellegrina , Edoardo Milotti

Phylogenetics uses alignments of molecular sequence data to learn about evolutionary trees relating species. Along branches, sequence evolution is modelled using a continuous-time Markov process characterised by an instantaneous rate…

We introduce a model for simulating mutation of prokaryote DNA sequences. Using that model we can then evaluated traditional techniques like parsimony and maximum likelihood methods for computing phylogenetic relationships. We also use the…

种群与进化 · 定量生物学 2007-05-23 Matthew J. Berryman , Andrew Allison , Derek Abbott

RNA secondary structure is modeled with the novel arbitrary-order hidden Markov model ({\alpha}-HMM). The {\alpha}-HMM extends over the traditional HMM with capability to model stochastic events that may be in influenced by historically…

生物大分子 · 定量生物学 2024-01-09 Sixiang Zhang , Aaron J. Yang , Liming Cai

The phase field model can accurately simulate the evolution of microstructures with complex morphologies, and it has been widely used for cell modeling in the last two decades. However, compared to other cellular models such as the…

生物物理 · 物理学 2022-06-13 Xiangyu Kuang , Guoye Guan , Chao Tang , Lei Zhang

Computational prediction of RNA structures is an important problem in computational structural biology. Studies of RNA structure formation often assume that the process starts from a fully synthesized sequence. Experimental evidence,…

生物大分子 · 定量生物学 2021-04-28 Vo Hong Thanh , Dani Korpela , Pekka Orponen

Elastic network models (ENMs) are valuable and efficient tools for characterizing the collective internal dynamics of proteins based on the knowledge of their native structures. The increasing evidence that the biological functionality of…

生物大分子 · 定量生物学 2015-09-01 Giovanni Pinamonti , Sandro Bottaro , Cristian Micheletti , Giovanni Bussi

Ab initio RNA secondary structure predictions have long dismissed helices interior to loops, so-called pseudoknots, despite their structural importance. Here, we report that many pseudoknots can be predicted through long time scales RNA…

生物物理 · 物理学 2009-11-10 A. Xayaphoummine , T. Bucher , F. Thalmann , H. Isambert

Energy landscapes provide a valuable means for studying the folding dynamics of short RNA molecules in detail by modeling all possible structures and their transitions. Higher abstraction levels based on a macro-state decomposition of the…

生物大分子 · 定量生物学 2014-09-09 Martin Mann , Marcel Kucharik , Christoph Flamm , Michael T. Wolfinger
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