相关论文: Computational TIRF enables optical sectioning beyo…

Total internal reflection fluorescence microscopy (TIRF) has enabled low-background, live-cell friendly imaging of cell surfaces and other thin samples thanks to the shallow penetration of the evanescent light field into the sample. The…

Evanescent-wave microscopy achieves sub-diffraction axial sectioning by confining fluorescence excitation to a thin layer close to the cell/substrate interface. How thin this light sheet exactly is, however, is often unknown. Particularly…

Traditional glass-based optics are typically optimized for narrow spectral bands, such as the visible (400-700nm) or shortwave infrared (1000-1800nm). While the emergence of VIS-SWIR sensors (400-1700nm) offers transformative potential,…

Multi-dielectric coated coverslip can be designed to reach large optical field enhancements when working under proper illumination conditions and in total internal reflection. In an objective-based total internal reflection fluorescence…

Measuring the three dimension nanoscale organization of protein or cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied…

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…

Acquiring accurate depth information of transparent objects using off-the-shelf RGB-D cameras is a well-known challenge in Computer Vision and Robotics. Depth estimation/completion methods are typically employed and trained on datasets with…

At the pinnacle of computational imaging is the co-optimization of camera and algorithm. This, however, is not the only form of computational imaging. In problems such as imaging through adverse weather, the bigger challenge is how to…

This paper presents an innovative approach to computational acoustic imaging of biperiodic surfaces, exploiting the capabilities of an acoustic superlens to overcome the diffraction limit. We address the challenge of imaging physical…

Mid-InfraRed spectroscopy is a promising label-free technique that can offer insights into morphological and pathological alterations in biological tissues at the molecular level. Owing to the development of the Fourier Transform InfraRed…

We report a novel generalized optical measurement system and computational approach to determine and correct aberrations in optical systems. We developed a computational imaging method capable of reconstructing an optical system's…

In recent years, computational Time-of-Flight (ToF) imaging has emerged as an exciting and a novel imaging modality that offers new and powerful interpretations of natural scenes, with applications extending to 3D, light-in-flight, and…

Reconstructing the high-fidelity surface from multi-view images, especially sparse images, is a critical and practical task that has attracted widespread attention in recent years. However, existing methods are impeded by the memory…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Image inpainting has achieved fundamental advances with deep learning. However, almost all existing inpainting methods aim to process natural images, while few target Thermal Infrared (TIR) images, which have widespread applications. When…

Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms. Ligands bind to…

While Fourier ptychography (FP) offers super-resolution for macroscopic imaging, its real-world application is severely hampered by atmospheric turbulence, a challenge largely unaddressed in existing macroscopic FP research operating under…

Imaging through optical multimode fibers (MMFs) has the potential to enable hair-thin endoscopes that reduce the invasiveness of imaging deep inside tissues and organs. Current approaches predominantly require active wavefront shaping and…