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Many researchers have been encouraged to describe the dynamics of chromosomal loci in chromatin using the classical Rouse model of polymer dynamics by the agreement between the measured mean square displacement (MSD) versus time of…

Active loop extrusion - the process of formation of dynamically growing chromatin loops due to the motor activity of DNA-binding protein complexes - is firmly established mechanism responsible for chromatin spatial organization at different…

统计力学 · 物理学 2023-01-11 Sergey Belan , Vladimir Parfenyev

While the structure of chromatin has been studied in great detail on length scales below 30 nm, amazingly little is known about the higher-order folding motifs of chromatin in interphase. Recent experiments give evidence that the folding…

软凝聚态物质 · 物理学 2009-02-16 Manfred Bohn , Dieter W. Heermann , Roel van Driel

The classic model of eukaryotic gene expression requires direct spatial contact between a distal enhancer and a proximal promoter. Recent Chromosome Conformation Capture (3C) studies show that enhancers and promoters are embedded in a…

基因组学 · 定量生物学 2014-11-25 Boryana Doyle , Geoffrey Fudenberg , Maxim Imakaev , Leonid A. Mirny

We investigate the conformational and dynamical properties of a partially active Rouse chain, where activity is localized within a specific segment, positioned at various locations along the chain and spanning any given length. Through…

软凝聚态物质 · 物理学 2024-12-06 Koushik Goswami , Norman Hsia , Cheng-Hung Chang , Hong-Yan Shih

We use Brownian dynamics simulations to study the formation of chromatin loops through diffusive sliding of slip-link-like proteins, mimicking the behaviour of cohesin molecules. We recently proposed that diffusive sliding is sufficient to…

软凝聚态物质 · 物理学 2020-11-02 Andrea Bonato , Chris A. Brackley , James Johnson , Davide Michieletto , Davide Marenduzzo

How long threadlike eukaryotic chromosomes fit tidily in the small volume of the nucleus without significant entanglement is just beginning to be understood, thanks to major advances in experimental techniques. Several polymer models, which…

软凝聚态物质 · 物理学 2024-10-03 D. Thirumalai , Guang Shi , Sucheol Shin , Changbong Hyeon

Polymer models are used to describe chromatin, which can be folded at different spatial scales by binding molecules. By folding, chromatin generates loops of various sizes. We present here a randomly cross-linked (RCL) polymer model, where…

软凝聚态物质 · 物理学 2017-05-12 Ofir Shukron , David Holcman

Chromatin loop extrusion is a popular model for the formation of CTCF loops and topological domains. Recent HiC data have revealed a strong bias in favour of a particular arrangement of the CTCF binding motifs that stabilize loops, and…

生物物理 · 物理学 2020-10-07 C. A. Brackley , J. Johnson , D. Michieletto , A. N. Morozov , M. Nicodemi , P. R. Cook , D. Marenduzzo

The population-averaged contact maps generated by the chromosome conformation capture technique provide important information about the average frequency of contact between pairs of chromatin loci as a function of the genetic distance…

统计力学 · 物理学 2024-04-09 Dmitry Starkov , Sergey Belan

We propose a model for the formation of chromatin loops based on the diffusive sliding of a DNA-bound factor which can dimerise to form a molecular slip-link. Our slip-links mimic the behaviour of cohesin-like molecules, which, along with…

生物物理 · 物理学 2017-10-04 C. A. Brackley , J. Johnson , D. Michieletto , A. N. Morozov , M. Nicodemi , P. R. Cook , D. Marenduzzo

The Rouse model can be regarded as the standard model to describe the dynamics of a short polymer chain under melt conditions. In this contribution, we explicitly check one of the fundamental assumptions of this model, namely that of a…

软凝聚态物质 · 物理学 2014-10-14 Diddo Diddens , Andreas Heuer

Chromatin and associated proteins constitute the highly folded structure of chromosomes. We consider a self-avoiding polymer model of the chromatin, segments of which may get cross-linked via protein binders that repel each other. The…

软凝聚态物质 · 物理学 2019-06-13 Amit Kumar , Debasish Chaudhuri

A living cell is an active environment where the organization and dynamics of chromatin are affected by different forms of activity. Optical experiments report that loci show subdiffusive dynamics and the chromatin fiber is seen to be…

软凝聚态物质 · 物理学 2023-03-15 Subhasish Chaki , Ligesh Theeyancheri , Rajarshi Chakrabarti

An active loop-extrusion mechanism is regarded as the main out--of--equilibrium mechanism responsible for the structuring of megabase-sized domains in chromosomes. We developed a model to study the dynamics of the chromosome fibre by…

生物大分子 · 定量生物学 2020-09-30 M. Crippa , Y. Zhan , G. Tiana

Motivated by the recent experimental observations of the DNA loop extrusion by protein motors, in this paper we investigate the statistical properties of the growing polymer loops within the ideal chain model. The loop conformation is…

统计力学 · 物理学 2021-04-30 Dmitry Starkov , Vladimir Parfenyev , Sergey Belan

The chromosome is a key player of cell physiology, and its dynamics provides valuable information about its physical organization. In both prokaryotes and eukaryotes, the short-time motion of chromosomal loci has been described as a Rouse…

生物物理 · 物理学 2018-02-28 K. E. Polovnikov , M. Gherardi , M. Cosentino-Lagomarsino , M. V. Tamm

The 3D folding of a mammalian gene can be studied by a polymer model, where the chromatin fibre is represented by a semiflexible polymer which interacts with multivalent proteins, representing complexes of DNA-binding transcription factors…

The formation of interface from an initial sharp interface in polydisperse A/B blends is studied using the external potential dynamic method. The present model is a nonlocal coupling model as we take into account the correlation between…

软凝聚态物质 · 物理学 2015-05-13 Shuanhu Qi , Xinghu Zhang , Dadong Yan

Chromatin looping is a major epigenetic regulatory mechanism in higher eukaryotes. Besides its role in transcriptional regulation, chromatin loops have been proposed to play a pivotal role in the segregation of entire chromosomes. The…

软凝聚态物质 · 物理学 2015-05-18 Manfred Bohn , Dieter W. Heermann
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