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Knowing a biomolecule's structure is inherently linked to and a prerequisite for any detailed understanding of its function. Significant effort has gone into developing technologies for structural characterization. These technologies do not…

数据结构与算法 · 计算机科学 2017-06-22 Michael Wegner , Oskar Taubert , Alexander Schug , Henning Meyerhenke

Curvilinear structures frequently appear in microscopy imaging as the object of interest. Crystallographic defects, i.e., dislocations, are one of the curvilinear structures that have been repeatedly investigated under transmission electron…

图像与视频处理 · 电气工程与系统科学 2021-10-19 Okan Altingövde , Anastasiia Mishchuk , Gulnaz Ganeeva , Emad Oveisi , Cecile Hebert , Pascal Fua

Cryo-electron microscopy is a technique in structural biology for discovering/determining the 3D structure of small molecules. A key step in this process is detecting common lines of intersection between unknown embedded image planes. We…

代数几何 · 数学 2014-02-25 David Dynerman

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the…

计算物理 · 物理学 2018-03-20 Amit Singer

Enhancing cryogenic electron microscopy (cryo-EM) 3D density maps at intermediate resolution (4-8 {\AA}) is crucial in protein structure determination. Recent advances in deep learning have led to the development of automated approaches for…

计算机视觉与模式识别 · 计算机科学 2025-05-16 Chenwei Zhang , Khanh Dao Duc

Electrical capacitance tomography (ECT) is a nonoptical imaging technique in which a map of the interior permittivity of a volume is estimated by making capacitance measurements at its boundary and solving an inverse problem. While previous…

计算机视觉与模式识别 · 计算机科学 2023-12-05 Manar Abdelatty , Joseph Incandela , Kangping Hu , Joseph W. Larkin , Sherief Reda , Jacob K. Rosenstein

Cryo-electron microscopy (cryo-EM) has emerged as a powerful technique for resolving the three-dimensional structures of macromolecules. A key challenge in cryo-EM is characterizing continuous heterogeneity, where molecules adopt a…

机器学习 · 统计学 2026-02-20 Roey Yadgar , Roy R. Lederman , Yoel Shkolnisky

Resolving the structural variability of proteins is often key to understanding the structure-function relationship of those macromolecular machines. Single particle analysis using Cryogenic electron microscopy (CryoEM), combined with…

定量方法 · 定量生物学 2022-11-22 Muyuan Chen , Bogdan Toader , Roy Lederman

One of the difficulties in 3D reconstruction of molecules from images in single particle Cryo-Electron Microscopy (Cryo-EM), in addition to high levels of noise and unknown image orientations, is heterogeneity in samples: in many cases, the…

计算机视觉与模式识别 · 计算机科学 2016-07-13 Roy R. Lederman , Amit Singer

Cryo-electron microscopy (cryo-EM) is a widely used technique for recovering the 3-D structure of biological molecules from a large number of experimentally generated noisy 2-D tomographic projection images of the 3-D structure, taken from…

数值分析 · 数学 2025-11-17 Jeremy Hoskins , Yuehaw Khoo , Oscar Mickelin , Amit Singer , Yuguan Wang

Single-particle cryo-electron microscopy (cryo-EM) has become one of the mainstream structural biology techniques because of its ability to determine high-resolution structures of dynamic bio-molecules. However, cryo-EM data acquisition…

机器学习 · 计算机科学 2022-04-18 Quanfu Fan , Yilai Li , Yuguang Yao , John Cohn , Sijia Liu , Seychelle M. Vos , Michael A. Cianfrocco

The central problem in cryo-electron microscopy (cryo-EM) is to recover the 3D structure from noisy 2D projection images which requires estimating the missing projection angles (poses). Recent methods attempted to solve the 3D…

计算机视觉与模式识别 · 计算机科学 2023-02-15 Lin Yao , Ruihan Xu , Zhifeng Gao , Guolin Ke , Yuhang Wang

Cryo-electron microscopy (cryo-EM) extracts single-particle density projections of individual biomolecules. Although cryo-EM is widely used for 3D reconstruction, due to its single-particle nature, it has the potential to provide…

Electron tomography offers important three-dimensional (3D) structural information which cannot be observed by two-dimensional imaging. By combining annular dark field scanning transmission electron microscopy (ADF-STEM) with aberration…

材料科学 · 物理学 2023-06-29 Juhyeok Lee , Moosung Lee , YongKeun Park , Colin Ophus , Yongsoo Yang

Cryo-electron microscopy provides 2-D projection images of the 3-D electron scattering intensity of many instances of the particle under study (e.g., a virus). Both symmetry (rotational point groups) and heterogeneity are important aspects…

应用统计 · 统计学 2019-05-17 Nan Xu , Peter C Doerschuk

Learning a disentangled, interpretable, and structured latent representation in 3D generative models of faces and bodies is still an open problem. The problem is particularly acute when control over identity features is required. In this…

计算机视觉与模式识别 · 计算机科学 2022-03-25 Simone Foti , Bongjin Koo , Danail Stoyanov , Matthew J. Clarkson

Four-dimensional scanning transmission electron microscopy (4D-STEM) of local atomic diffraction patterns is emerging as a powerful technique for probing intricate details of atomic structure and atomic electric fields. However, efficient…

图像与视频处理 · 电气工程与系统科学 2019-01-15 Xin Li , Ondrej E. Dyck , Mark P. Oxley , Andrew R. Lupini , Leland McInnes , John Healy , Stephen Jesse , Sergei V. Kalinin

Particle picking is currently a critical step in the cryo-electron microscopy single particle reconstruction pipeline. Contaminations in the acquired micrographs severely degrade the performance of particle pickers, resulting is many…

计算机视觉与模式识别 · 计算机科学 2022-01-19 Amitay Eldar , Ido Amos , Yoel Shkolnisky

Atomic electron tomography (AET) enables the determination of 3D atomic structures by acquiring a sequence of 2D tomographic projection measurements of a particle and then computationally solving for its underlying 3D representation.…

图像与视频处理 · 电气工程与系统科学 2025-12-18 Nalini M. Singh , Tiffany Chien , Arthur R. C. McCray , Colin Ophus , Laura Waller

The missing phase problem in X-ray crystallography is commonly solved using the technique of molecular replacement, which borrows phases from a previously solved homologous structure, and appends them to the measured Fourier magnitudes of…

计算机视觉与模式识别 · 计算机科学 2017-04-27 Tejal Bhamre , Teng Zhang , Amit Singer