相关论文: Two-photon MINFLUX with doubled localization preci…
Point Spread Function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline…
In wide-field nonlinear microscopy, wavefront modulation by means of phase-only spatial light modulators (SLMs) allows achieving simultaneous two-photon excitation and fluorescence emission from specific region-of-interests (ROIs) of…
Light penetration depth in biological tissue is limited by tissue scattering. Correcting scattering becomes particularly challenging in scenarios with limited photon availability and when access to the transmission side of the scattering…
Biological and biomedical samples are routinely examined using focused two-photon (2P) fluorescence microscopy due to its intrinsic axial sectioning and reduced out-of-focus bleaching. However, 2P imaging often requires excitation…
We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During…
We closely replicated and extended a recent experiment ("Spatial properties of entangled two-photon absorption," Phys. Rev. Lett. 129, 183601, 2022) that reportedly observed enhancement of two-photon absorption rates in molecular samples by…
The research on recognizing the most discriminative regions provides referential information for weakly supervised object localization with only image-level annotations. However, the most discriminative regions usually conceal the other…
Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…
Due to limited size and imperfect of the optical components in a spectrometer, aberration has inevitably been brought into two-dimensional multi-fiber spectrum image in LAMOST, which leads to obvious spacial variation of the point spread…
The localisation of fluorophores is an important aspect of the determination of the biological function of cellular systems. Quantum correlation microscopy (QCM) is a promising technique for providing diffraction unlimited emitter…
We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…
Two-photon excited fluorescence (TPEF) is a standard technique in modern microscopy but still affected by photo-damage of the probe. It was proposed that TPEF can be enhanced by using entangled photons, but has proven to be challenging.…
Evanescent light excitation is widely used in super-resolution fluorescence microscopy to confine light and reduce background noise. Herein we propose a method of exploiting evanescent light in the context of emission. When a fluorophore is…
We show that the modulation of the phases of the laser beams of ultra-short pulses leads to modulation of the two photon fluorescence intensity. The phase modulation technique when used in multi-photon microscopy can improve the signal to…
Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…
We investigate the prospects of using two-mode intensity squeezed twin-beams, generated in Rb vapor, to improve the sensitivity of spectroscopic measurements by engaging two-photon Raman transitions. As a proof of principle demonstration,…
Recently, millimeter-wave (mmWave) 5G localization has been shown to be to provide centimeter-level accuracy, lending itself to many location-aware applications, e.g., connected autonomous vehicles (CAVs). One assumption usually made in the…
A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…
We present a novel approach to achieve hyper spectral resolution, high sensitive detection, and high speed data acquisition Stimulated Raman Spectroscopy by employing amplified offset-phase controlled fs-pulse bursts. In this approach, the…
A dual-excitation method for resonant-frequency tracking in scanning probe microscopy based on amplitude detection is developed. This method allows the cantilever to be operated at or near resonance for techniques where standard phase…