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We summarize the recent simulation progress of micromanipulation experiments on RNAs. Our work mainly consults with two important small RNAs unfolding experiments carried out by Bustamante group. Our results show that, in contrast to…

软凝聚态物质 · 物理学 2007-05-23 Fei Liu , Zhong-can Ou-Yang

The description of nonequilibrium processes in nano-sized objects, where the typical energies involved are a few times, is increasingly becoming central to disciplines as diverse as condensed-matter physics, materials science, and…

统计力学 · 物理学 2009-11-11 D. Collin , F. Ritort , C. Jarzynski , S. B. Smith , I. Tinoco , C. Bustamante

We consider in this paper, a few important issues in non-equilibrium work fluctuations and their relations to equilibrium free energies. First we show that Jarzynski identity can be viewed as a cumulant expansion of work. For a switching…

统计力学 · 物理学 2015-05-20 M Suman Kalyan , G Anjan Prasad , V S S Sastry , K P N Murthy

RNA folding is a kinetic process governed by the competition of a large number of structures stabilized by the transient formation of base pairs that may induce complex folding pathways and the formation of misfolded structures. Despite of…

生物物理 · 物理学 2009-03-16 M. Manosas , I. Junier , F. Ritort

When a system is in equilibrium, external perturbations yield a time series of non-equilibrium distributions, and recent experimental techniques give access to the non-equilibrium data that may contain critical information. Jinwoo and…

统计力学 · 物理学 2023-01-16 Lee Jinwoo

By exerting mechanical force it is possible to unfold/refold RNA molecules one at a time. In a small range of forces, an RNA molecule can hop between the folded and the unfolded state with force-dependent kinetic rates. Here, we introduce a…

生物物理 · 物理学 2012-08-27 M. Manosas , J. -D. Wen , P. T. X. Li , S. B. Smith , C. Bustamante , I. Tinoco, , F. Ritort

A simple zipper model is introduced, representing in a simplified way, e.g., the folded DNA double helix or hairpin structures in RNA. The double stranded hairpin is connected to a heat bath at temperature $T$ and subject to an external…

统计力学 · 物理学 2024-01-18 P. Werner , A. K. Hartmann , S. N. Majumdar

We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two state manner under the action of mechanical force. We apply path thermodynamics to derive analytical expressions for the average…

统计力学 · 物理学 2009-02-25 Maria Manosas , Alessandro Mossa , Nuria Forns , Josep Maria Huguet , Felix Ritort

We introduce a simple enhanced sampling approach for the calculation of free energy differences and barriers along a one-dimensional reaction coordinate. First, a small number of short nonequilibrium simulations are carried out along the…

化学物理 · 物理学 2021-11-03 Kristof M. Bal

We study theoretically the denaturation of single RNA molecules by mechanical stretching, focusing on signatures of the (un)folding pathway in molecular fluctuations. Our model describes the interactions between nucleotides by incorporating…

软凝聚态物质 · 物理学 2009-11-07 Ulrich Gerland , Ralf Bundschuh , Terence Hwa

Equilibrium thermodynamics is combined with Jarzynski's irreversible work theorem to quantify the excess entropy produced by irreversible processes. The resulting rectified form of the second law parallels the first law, in the sense that…

统计力学 · 物理学 2007-05-23 Dor Ben-Amotz , J. M. Honig

The mechanical unfolding of a simple RNA hairpin and of a 236--bases portion of the Tetrahymena thermophila ribozyme is studied by means of an Ising--like model. Phase diagrams and free energy landscapes are computed exactly and suggest a…

软凝聚态物质 · 物理学 2009-11-05 A. Imparato , A. Pelizzola , M. Zamparo

The Jarzynski equality and the fluctuation theorem relate equilibrium free energy differences to non-equilibrium measurements of the work. These relations extend to single-molecule experiments that have probed the finite-time thermodynamics…

统计力学 · 物理学 2008-08-30 Paul Maragakis , Felix Ritort , Carlos Bustamante , Martin Karplus , Gavin E. Crooks

We investigate the work dissipated during the irreversible unfolding of single molecules by mechanical force, using the simplest model necessary to represent experimental data. The model consists of two levels (folded and unfolded states)…

生物物理 · 物理学 2012-08-27 F. Ritort , C. Bustamante , I. Tinoco,

A system of a solvated canonical B-DNA of 12 base pairs with the specified sequence is initially equilibrated in a state of zero external force $f$ acting on it. After equilibration, a switching experiment is performed over the system by…

软凝聚态物质 · 物理学 2025-08-12 S. Siva Nasarayya Chari , Prabal K. Maiti

Rare event simulation and estimation for systems in equilibrium are among the most challenging topics in molecular dynamics. As was shown by Jarzynski and others, nonequilibrium forcing can theoretically be used to obtain equilibrium rare…

最优化与控制 · 数学 2015-06-11 Carsten Hartmann , Christof Schütte

We consider the paradigm of an overdamped Brownian particle in a potential well, which is modulated through an external protocol, in the presence of stochastic resetting. Thus, in addition to the short range diffusive motion, the particle…

统计力学 · 物理学 2020-03-25 Deepak Gupta , Carlos A. Plata , Arnab Pal

Nonequilibrium experiments of single biomolecules such as force-induced unfolding reveal details about a few degrees of freedom of a complex system. Molecular dynamics simulations can provide complementary information, but exploration of…

RNA duplex stability depends strongly on ionic conditions, and inside cells RNAs are exposed to both monovalent and multivalent ions. Despite recent advances, we do not have general methods to quantitatively account for the effects of…

生物大分子 · 定量生物学 2013-07-02 C. V. Bizarro , A. Alemany , F. Ritort

Using coarse-grained model we have explored forced-unfolding of RNA hairpin as a function of $f_S$ and the loading rate ($r_f$). The simulations and theoretical analysis have been done without and with the handles that are explicitly…

生物大分子 · 定量生物学 2009-11-13 Changbong Hyeon , D. Thirumalai
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