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Toxoplasma gondii is a parasitic protozoan that causes dis- seminated toxoplasmosis, a disease that afflicts roughly a third of the worlds population. Its virulence is predicated on its motility and ability to enter and exit nucleated…

计算机视觉与模式识别 · 计算机科学 2018-01-15 Mojtaba S. Fazli , Stephen A. Vella , Silvia N. J. Moreno , Shannon Quinn

Toxoplasma gondii is the causative agent responsible for toxoplasmosis and serves as one of the most common parasites in the world. For a successful lytic cycle, T. gondii must traverse biological barriers in order to invade host cells, and…

定量方法 · 定量生物学 2017-08-21 Mojtaba Sedigh Fazli , Stephen Andrew Vella , Silvia N. J. Moreno , Shannon Quinn

T cells use sophisticated shape dynamics (morphodynamics) to migrate towards and neutralise infected and cancerous cells. However, there is limited quantitative understanding of the migration process in 3D extracellular matrices (ECMs) and…

生物物理 · 物理学 2022-04-11 Henry Cavanagh , Daryan Kempe , Jessica K. Mazalo , Maté Biro , Robert G. Endres

Tracking many cells in time-lapse 3D image sequences is an important challenging task of bioimage informatics. Motivated by a study of brain-wide 4D imaging of neural activity in C. elegans, we present a new method of multi-cell tracking.…

计算机视觉与模式识别 · 计算机科学 2018-01-18 Osamu Hirose , Shotaro Kawaguchi , Terumasa Tokunaga , Yu Toyoshima , Takayuki Teramoto , Sayuri Kuge , Takeshi Ishihara , Yuichi Iino , Ryo Yoshida

Mathematical models have been widely used to describe the collective movement of bacteria by chemotaxis. In particular, bacterial concentration waves traveling in a narrow channel have been experimentally observed and can be precisely…

偏微分方程分析 · 数学 2016-04-15 Casimir Emako , Charlène Gayrard , Axel Buguin , Luís Neves de Almeida , Nicolas Vauchelet

3D cell tracking in a living organism has a crucial role in live cell image analysis. Cell tracking in C. elegans has two difficulties. First, cell migration in a consecutive frame is large since they move their head during scanning.…

计算机视觉与模式识别 · 计算机科学 2024-03-21 Kaito Shiku , Hiromitsu Shirai , Takeshi Ishihara , Ryoma Bise

We introduce a device developed to perform a 3D tracking of passive or active particles under flow, confined in a medium of hundreds micrometers wide. Micro-objects are placed inside a vertical glass capillary and two mirrors are set behind…

软凝聚态物质 · 物理学 2025-06-09 J. Moscatelli , X Benoit Gonin , F. Elias

Parkinson's disease (PD) is a common neurodegenerative disease with a high degree of heterogeneity in its clinical features, rate of progression, and change of variables over time. In this work, we present a novel data-driven, network-based…

应用统计 · 统计学 2020-07-01 Sanjukta Krishnagopal , Rainer Von Coelln , Lisa M. Shulman , Michelle Girvan

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…

流体动力学 · 物理学 2017-09-19 S. Quint , A. F. Christ , A. Guckenberger , S. Himbert , L. Kaestner , S. Gekle , C. Wagner

The movement of the infective form of the T. Cruzi parasite within the human blood is not completely understood. Video microscopy observations confirm forward motility of the protozoa and relate it to the deformation of the body,…

生物物理 · 物理学 2025-10-27 Alberto-Mario Castillo , Gabriel Villalobos

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

图像与视频处理 · 电气工程与系统科学 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

Bacteria living on surfaces use different types of motility mechanisms to move on the surface in search of food or to form micro-colonies. Twitching is one such form of motility employed by bacteria such as N. gonorrhoeae, in which the…

生物物理 · 物理学 2020-04-29 Konark Bisht , Rahul Marathe

We propose a scalable track-before-detect (TBD) tracking method based on a Poisson/multi-Bernoulli model. To limit computational complexity, we approximate the exact multi-Bernoulli mixture posterior probability density function (pdf) by a…

信号处理 · 电气工程与系统科学 2021-09-06 Thomas Kropfreiter , Jason L. Williams , Florian Meyer

We seek to characterize the motility of mouse fibroblasts on 2D substrates. Utilizing automated tracking techniques, we find that cell trajectories are super-diffusive, where displacements scale faster than t^(1/2) in all directions. Two…

软凝聚态物质 · 物理学 2017-12-15 G Passucci , ME Brasch , JH Henderson , V Zaburdaev , ML Manning

The nematode Caenorhabditis elegans (C. elegans) is used as a model organism to better understand developmental biology and neurobiology. C. elegans features an invariant cell lineage, which has been catalogued and observed using…

计算机视觉与模式识别 · 计算机科学 2022-08-03 Andrew Lauziere , Ryan Christensen , Hari Shroff

Locomotion is essential for living cells. It enables bacteria and algae to explore space for food, cancer to spread, and immune system to fight infections. Motile cells display trajectories of intriguing complexity, from regular (e.g.…

软凝聚态物质 · 物理学 2021-12-28 C. Misbah , M. S. Rizvi , W. F. Hu , T. S. Lin , S. Rafai , A. Farutin

The persistent motion of bacteria produces clusters with a stationary cluster size distribution (CSD). Here we develop a minimal model for bacteria in a narrow channel to assess the relative importance of motility diversity (i.e.…

软凝聚态物质 · 物理学 2020-11-19 Pablo de Castro , Saulo Diles , Rodrigo Soto , Peter Sollich

Trypanosoma brucei (T. brucei), a single-celled parasite and natural microswimmer, is responsible for fatal sleeping sickness in infected mammals, including humans. Understanding how T. brucei interacts with fluid environments and navigates…

软凝聚态物质 · 物理学 2025-06-02 Zihan Tan , Julian I. U. Peters , Holger Stark

We present a fast, high-throughput method for characterizing the motility of microorganisms in 3D based on standard imaging microscopy. Instead of tracking individual cells, we analyse the spatio-temporal fluctuations of the intensity in…

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

计算机视觉与模式识别 · 计算机科学 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury
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