相关论文: Selective intracellular delivery and intracellular…
In electrophysiology, multielectrode array devices (MEA) are the gold standard for the study of large ensambles of electrogenic cells. In the last decades, thanks to the adoption of nanotechnologies, the study of physiological and…
This PhD thesis introduces the concept of meta-electrodes coupled with laser optoporation for high quality intracellular signals from hiPSCs-CM. These signals can be recorded on high-density commercial CMOS-MEAs from 3Brain characterized by…
Intracellular access with high spatiotemporal resolution can enhance our understanding of how neurons or cardiomyocytes regulate and orchestrate network activity, and how this activity can be affected with pharmacology or other…
Mesoscale molecular assemblies on the cell surface, such as cilia and filopodia, integrate information, control transport and amplify signals. Synthetic devices mimicking these structures could sensitively monitor these cellular functions…
Numerical investigation of the interaction of electromagnetic fields with eukaryotic cells requires specifically adapted computer models. Virtual microdosimetry, used to investigate exposure, requires volumetric cell models, which are…
Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined…
We present an electrophoretic platform based on 3D hollow nanoelectrodes capable of controlling and quantifying the intracellular delivery of single nanoparticles in single selected cells by surface-enhanced Raman spectroscopy (SERS). The…
Extracellular, large scale in vivo recording of neural activity is mandatory for elucidating the interaction of neurons within large neural networks at the level of their single unit activity. Technological achievements in MEMS-based…
Intracellular recordings of neuronal membrane potential are a central tool in neurophysiology. In many situations, especially in vivo, the traditional limitation of such recordings is the high electrode resistance, which may cause…
The formation and dissociation of specific noncovalent interactions between a variety of macromolecules play a crucial role in the function of biological systems. During the last few years, three main lines of research led to a dramatic…
A technology for recording electrical activity of large neuron populations at arbitrary depth in brain tissues with less than cell spatial and millisecond temporal resolutions was the most craving dream of neuroscientists and a long pursued…
Molecular communications (MC), where molecules are used to encode, transmit, and receive information, is a promising means of enabling the coordination of nanoscale devices. The paradigm has been extensively studied from various aspects,…
Multimodal single-cell technologies enable the simultaneous collection of diverse data types from individual cells, enhancing our understanding of cellular states. However, the integration of these datatypes and modeling the…
In the 21st century, biosensors have gathered much wider attention than ever before, irrespective of the technology that promises to bring them forward. With the recent COVID-19 outbreak, the concern and efforts to restore global health and…
The recent advancements in bio-engineering and wireless communications systems have motivated researchers to propose novel applications for telemedicine, therapeutics and human health monitoring. For instance, through wireless medical…
Optical biosensors are indispensable in medical and environmental diagnostics, yet existing approaches are fundamentally limited in their sensitivity due to ensemble-averaged measurements. Digital biosensing has emerged as a promising…
Multi-Electrode Arrays (MEAs) have been widely used to record neuronal activities, which could be used in the diagnosis of gene defects and drug effects. In this paper, we address the problem of classifying in vitro MEA recordings of mouse…
Biological tissue integrity is actively maintained by cells. It is essential to comprehend how cells accomplish this in order to stage tissue diseases. However, addressing the complexity of a cell's system of interrelated mechanisms poses a…
Biological cells, by definition, are the basic units which contain the fundamental molecules of life of which all living things are composed. Understanding how they function and differentiating cells from one another therefore is of…
In-vivo, cells are frequently exposed to multiple mechanical stimuli arising from the extracellular microenvironment, with deep impact on many biological functions. On the other hand, current methods for mechanobiology do not allow to…