相关论文: Fluorescence decay data analysis correcting for de…
RAndom Temporal Signals (RATS) method has proven to be a useful and versatile method for measuring photoluminescence (PL) dynamics and fluorescence lifetime imaging (FLIM). Here, we present two fundamental development steps in the method.…
Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…
A formalism for treating the pile-up produced in solid-state detectors by laser-driven pulsed x-ray sources has been developed. It allows the direct use of x-ray spectroscopy without artificially decreasing the number of counts in the…
Quality control in molecular optical sectioning microscopy is indispensable for transforming acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed,…
Scalable atom-based quantum platforms for simulation, computing, and metrology require fast high-fidelity, low-loss imaging of individual atoms. Standard fluorescence detection methods rely on continuous cooling, limiting the detection…
Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…
We present a comprehensive study addressing pile-up effects in single photoelectron counting with R-11265 Hamamatsu multi-anode photomultiplier tubes (MAPMTs) equipped with the SPACIROC-3 ASIC. Extended dead time in the electronics causes…
Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…
Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…
We consider a density estimation problem arising in nuclear physics. Gamma photons are impinging on a semiconductor detector, producing pulses of current. The integral of this pulse is equal to the total amount of charge created by the…
Frequency-filtered photon correlations have been proven to be extremely useful in grasping how the detection process alters photon statistics. Harnessing the spectral correlations also permits refinement of the emission and unraveling of…
We demonstrate an electro-optic wide-field method to enable fluorescence lifetime microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven Pockels cells are used to efficiently gate images at 39 MHz,…
The widely used experimental technique of continuous-wave detection assumes counting pulses of photocurrent from a click-type detector inside a given measurement time window. With such a procedure we miss out the photons detected after each…
Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…
We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…
Pulse pile-up in pulse-height energy analyzers increases when the incident rate of pulses increases relative to the inverse of the dead time per pulse of the detection system. Changes in the observed energy distributions with incident rate…
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…
In X-ray astronomy, most observatories utilize multi-pixel photon-counting devices. When a photon counting device observes a bright source, we face an unavoidable problem called pile-up. Pile-up leads to mistakes in the observational…
Time-resolved photoluminescence (PL) is commonly used to track dynamics in a broad range of materials. Thus, the search for simplification of the acquisition of PL kinetics attracts continuous attention. This paper presents a new robust and…