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Serial section Microscopy is an established method for volumetric anatomy reconstruction. Section series imaged with Electron Microscopy are currently vital for the reconstruction of the synaptic connectivity of entire animal brains such as…

计算机视觉与模式识别 · 计算机科学 2016-06-20 Philipp Hanslovsky , John A. Bogovic , Stephan Saalfeld

State-of-the-art electron microscopes such as scanning electron microscopes (SEM), scanning transmission electron microscopes (STEM) and transmission electron microscopes (TEM) have become increasingly sophisticated. However, the quality of…

计算物理 · 物理学 2023-03-31 I. Lobato , T. Friedrich , S. Van Aert

A central problem in neuroscience is reconstructing neuronal circuits on the synapse level. Due to a wide range of scales in brain architecture such reconstruction requires imaging that is both high-resolution and high-throughput. Existing…

计算机视觉与模式识别 · 计算机科学 2012-10-03 Tao Hu , Juan Nunez-Iglesias , Shiv Vitaladevuni , Lou Scheffer , Shan Xu , Mehdi Bolorizadeh , Harald Hess , Richard Fetter , Dmitri Chklovskii

The most established method of reconstructing neural circuits from animals involves slicing tissue very thin, then taking mosaics of electron microscope (EM) images. To trace neurons across different images and through different sections,…

定量方法 · 定量生物学 2013-04-23 Louis K. Scheffer , Bill Karsh , Shiv Vitaladevun

The alignment of serial-section electron microscopy (ssEM) images is critical for efforts in neuroscience that seek to reconstruct neuronal circuits. However, each ssEM plane contains densely packed structures that vary from one section to…

计算机视觉与模式识别 · 计算机科学 2017-12-06 Inwan Yoo , David G. C. Hildebrand , Willie F. Tobin , Wei-Chung Allen Lee , Won-Ki Jeong

Serial section electron microscopy (ssEM) is a widely used technique for obtaining volumetric information of biological tissues at nanometer scale. However, accurate 3D reconstructions of identified cellular structures and volumetric…

图像与视频处理 · 电气工程与系统科学 2020-02-06 Thanuja D. Ambegoda , Julien N. P. Martel , Jozef Adamcik , Matthew Cook , Richard H. R. Hahnloser

Nowadays, modern electron microscopes deliver images at atomic scale. The precise atomic structure encodes information about material properties. Thus, an important ingredient in the image analysis is to locate the centers of the atoms…

计算机视觉与模式识别 · 计算机科学 2017-09-13 Benjamin Berkels , Benedikt Wirth

Comprehensive, synapse-resolution imaging of the brain will be crucial for understanding neuronal computations and function. In connectomics, this has been the sole purview of volume electron microscopy (EM), which entails an excruciatingly…

图像与视频处理 · 电气工程与系统科学 2023-03-03 Yicong Li , Yaron Meirovitch , Aaron T. Kuan , Jasper S. Phelps , Alexandra Pacureanu , Wei-Chung Allen Lee , Nir Shavit , Lu Mi

Neuron segmentation in electron microscopy (EM) aims to reconstruct the complete neuronal connectome; however, current deep learning-based methods are limited by their reliance on large-scale training data and extensive, time-consuming…

计算机视觉与模式识别 · 计算机科学 2026-01-23 Liuyun Jiang , Yanchao Zhang , Jinyue Guo , Yizhuo Lu , Ruining Zhou , Hua Han

Scanning Electron Microscopy (SEM) is indispensable in modern materials science, enabling high-resolution imaging across a wide range of structural, chemical, and functional investigations. However, SEM imaging remains constrained by…

Electron microscopy (EM) images exhibit anisotropic axial resolution due to the characteristics inherent to the imaging modality, presenting challenges in analysis and downstream tasks.In this paper, we propose a diffusion-model-based…

计算机视觉与模式识别 · 计算机科学 2023-08-04 Kyungryun Lee , Won-Ki Jeong

Scanning transmission electron microscopy (STEM) has become the technique of choice for quantitative characterization of atomic structure of materials, where the minute displacements of atomic columns from high-symmetry positions can be…

材料科学 · 物理学 2021-10-05 Kevin M. Roccapriore , Nicole Creange , Maxim Ziatdinov , Sergei V. Kalinin

The detailed reconstruction of neural anatomy for connectomics studies requires a combination of resolution and large three-dimensional data capture provided by serial section electron microscopy (ssEM). The convergence of high throughput…

计算机视觉与模式识别 · 计算机科学 2017-11-21 Arthur W. Wetzel , Jennifer Bakal , Markus Dittrich , David G. C. Hildebrand , Josh L. Morgan , Jeff W. Lichtman

Electron microscopy (EM) imaging offers unparalleled resolution for analyzing neural tissues, crucial for uncovering the intricacies of synaptic connections and neural processes fundamental to understanding behavioral mechanisms. Recently,…

计算机视觉与模式识别 · 计算机科学 2024-08-27 Ruohua Shi , Qiufan Pang , Lei Ma , Lingyu Duan , Tiejun Huang , Tingting Jiang

Morphology of mitochondria plays critical roles in mediating their physiological functions. Accurate segmentation of mitochondria from 3D electron microscopy (EM) images is essential to quantitative characterization of their morphology at…

计算机视觉与模式识别 · 计算机科学 2022-06-16 Yunpeng Xiao , Youpeng Zhao , Ge Yang

Instance segmentation in electron microscopy (EM) volumes is tough due to complex shapes and sparse annotations. Self-supervised learning helps but still struggles with intricate visual patterns in EM. To address this, we propose a…

计算机视觉与模式识别 · 计算机科学 2023-09-06 Yinda Chen , Wei Huang , Xiaoyu Liu , Shiyu Deng , Qi Chen , Zhiwei Xiong

Electron microscopy (EM) enables the reconstruction of neural circuits at the level of individual synapses, which has been transformative for scientific discoveries. However, due to the complex morphology, an accurate reconstruction of…

Neural circuits can be reconstructed from brain images acquired by serial section electron microscopy. Image analysis has been performed by manual labor for half a century, and efforts at automation date back almost as far. Convolutional…

计算机视觉与模式识别 · 计算机科学 2019-05-01 Kisuk Lee , Nicholas Turner , Thomas Macrina , Jingpeng Wu , Ran Lu , H. Sebastian Seung

Identifying complex neural circuitry from electron microscopic (EM) images may help unlock the mysteries of the brain. However, identifying this circuitry requires time-consuming, manual tracing (proofreading) due to the size and intricacy…

定量方法 · 定量生物学 2014-09-04 Stephen M. Plaza

We report resolution enhancement in scanning electron microscopy (SEM) images using a generative adversarial network. We demonstrate the veracity of this deep learning-based super-resolution technique by inferring unresolved features in…

计算机视觉与模式识别 · 计算机科学 2019-08-21 Kevin de Haan , Zachary S. Ballard , Yair Rivenson , Yichen Wu , Aydogan Ozcan
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