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Molecular chaperones are machines that consume copious amount of ATP to drive misfolded proteins or RNA to fold into functionally competent native states. Because the folding landscapes of biomolecules with complex native state topology are…

生物物理 · 物理学 2025-06-17 Changbong Hyeon , D. Thirumalai

Molecular chaperones are ATP-consuming biological machines, which facilitate the folding of proteins and RNA molecules that are kinetically trapped in misfolded states for long times. Unassisted folding occurs by the kinetic partitioning…

生物大分子 · 定量生物学 2019-09-18 D. Thirumalai , George H. Lorimer , Changbong Hyeon

RNA folding is a kinetic process governed by the competition of a large number of structures stabilized by the transient formation of base pairs that may induce complex folding pathways and the formation of misfolded structures. Despite of…

生物物理 · 物理学 2009-03-16 M. Manosas , I. Junier , F. Ritort

The kinetic folding of RNA sequences into secondary structures is modeled as a complex adaptive system, the components of which are possible RNA structural rearrangements (SRs) and their associated bases and base pairs. RNA bases and base…

生物大分子 · 定量生物学 2007-05-23 Wilfred Ndifon

Chaperonins are biological nanomachines that help newly translated proteins to fold by rescuing them from kinetically trapped misfolded states. Protein folding assistance by the chaperonin machinery is obligatory in vivo for a subset of…

生物大分子 · 定量生物学 2022-11-29 George Stan , George H. Lorimer , D. Thirumalai

We study the statistical mechanics of RNA secondary structures designed to have an attraction between two different types of structures as a model system for heteropolymer aggregation. The competition between the branching entropy of the…

生物物理 · 物理学 2009-11-11 Vishwesha Guttal , Ralf Bundschuh

The folding of RNA and DNA strands plays crucial roles in biological systems and bionanotechnology. However, studying these processes with high-resolution numerical models is beyond current computational capabilities due to the timescales…

软凝聚态物质 · 物理学 2024-02-07 F. Tosti Guerra , E. Poppleton , P. Šulc , L. Rovigatti

RNAs self-interact through hydrogen-bond base-pairing between nucleotides and fold into specific, stable structures that substantially govern their biochemical behaviour. Experimental characterization of these structures remains difficult,…

量子物理 · 物理学 2023-05-02 Tristan Zaborniak , Juan Giraldo , Hausi Müller , Hosna Jabbari , Ulrike Stege

Atomically detailed simulations of RNA folding have proven very challenging in view of the difficulties of developing realistic force fields and the intrinsic computational complexity of sampling rare conformational transitions. To tackle…

软凝聚态物质 · 物理学 2022-05-26 Gianmarco Lazzeri , Cristian Micheletti , Samuela Pasquali , Pietro Faccioli

We introduce a method for predicting RNA folding pathways, with an application to the most important RNA tetraloops. The method is based on the idea that ensembles of three-dimensional fragments extracted from high-resolution crystal…

生物大分子 · 定量生物学 2016-11-21 Sandro Bottaro , Alejandro Gil-Ley , Giovanni Bussi

RNA molecules are essential cellular machines performing a wide variety of functions for which a specific three-dimensional structure is required. Over the last several years, experimental determination of RNA structures through X-ray…

生物大分子 · 定量生物学 2015-06-11 Tristan Cragnolini , Philippe Derreumaux , Samuela Pasquali

We present a thermodynamically robust coarse-grained model to simulate folding of RNA in monovalent salt solutions. The model includes stacking, hydrogen bond and electrostatic interactions as fundamental components in describing the…

生物大分子 · 定量生物学 2013-04-16 Natalia A. Denesyuk , D. Thirumalai

The increasing importance of RNA as a prime player in biology can hardly be overstated. Problems in RNA, such as folding and RNA-RNA interactions that drive phase separation, require cations. Because experiments alone cannot reveal the…

生物大分子 · 定量生物学 2025-06-10 D. Thirumalai , Naoto Hori , Hung T. Nguyen

Computational prediction of RNA structures is an important problem in computational structural biology. Studies of RNA structure formation often assume that the process starts from a fully synthesized sequence. Experimental evidence,…

生物大分子 · 定量生物学 2021-04-28 Vo Hong Thanh , Dani Korpela , Pekka Orponen

We present a solvable model that predicts the folding kinetics of two-state proteins from their native structures. The model is based on conditional chain entropies. It assumes that folding processes are dominated by small-loop closure…

生物大分子 · 定量生物学 2007-05-23 Thomas R. Weikl , Matteo Palassini , Ken A. Dill

We present a panoramic view of the utility of coarse-grained (CG) models to study folding and functions of proteins and RNA. Drawing largely on the methods developed in our group over the last twenty years, we describe a number of key…

生物大分子 · 定量生物学 2008-08-25 D. L. Pincus , S. S. Cho , C. Hyeon , D. Thirumalai

Non-coding RNA sequences play a great role in controlling a number of cellular functions, thus raising the need to understand their complex conformational dynamics in quantitative detail. In this perspective, we first show that single…

生物大分子 · 定量生物学 2012-11-29 Jong-Chin Lin , Changbong Hyeon , D. Thirumalai

The computer-aided folding of biomolecules, particularly RNAs, is one of the most difficult challenges in computational structural biology. RNA tetraloops are fundamental RNA motifs playing key roles in RNA folding and RNA-RNA and…

生物大分子 · 定量生物学 2016-09-28 Petra Kührová , Robert B. Best , Sandro Bottaro , Giovanni Bussi , Jiří Šponer , Michal Otyepka , Pavel Banáš

The translocation of structured RNA or DNA molecules through narrow pores necessitates the opening of all base pairs. Here, we study the interplay between the dynamics of translocation and base-pairing theoretically, using kinetic Monte…

生物大分子 · 定量生物学 2009-11-11 Ralf Bundschuh , Ulrich Gerland

Nowadays different experimental techniques, such as single molecule or relaxation experiments, can provide dynamic properties of biomolecular systems, but the amount of detail obtainable with these methods is often limited in terms of time…

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