嵌入分子诱导的DNA熵弹性转变
生物物理
2009-11-13 v1 化学物理
摘要
我们使用光镊对DNA分子与可嵌入DNA分子的药物柔红霉素和溴化乙锭相互作用时进行拉伸实验。这些实验在从零到2 pN的低力区间内进行。我们的结果表明，随着药物浓度增加至某一临界值，DNA-药物复合物的持久长度强烈增加。超过该临界值后，持久长度急剧下降，并在更高药物浓度下基本保持恒定。分子的围长随药物浓度增加而单调增加并趋于饱和。测量得到的嵌入剂临界浓度与先前由沉降实验获得的DNA-药物复合物螺旋-线团转变的报道值一致。
引用
@article{arxiv.0704.2346,
title = {Transition on the entropic elasticity of DNA induced by intercalating molecules},
author = {M. S. Rocha and M. C. Ferreira and O. N. Mesquita},
journal= {arXiv preprint arXiv:0704.2346},
year = {2009}
}
备注
This experimental article shows and discuss a transition observed in the persistence length of DNA molecules when studied as a function of some intercalating drug concentrations, like daunomycin and ethidium bromide. It has 15 pages and 4 figures. The article presented here is in preprint format