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Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

Diffuse speckle contrast analysis (DSCA), also called speckle contrast optical spectroscopy(SCOS), has emerged as a groundbreaking optical imaging technique for tracking dynamic biological processes, including blood flow and tissue…

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Conventional spectrometer and polarimeter systems rely on bulky optics, fundamentally limiting compact integration and hindering multi-dimensional optical sensing capabilities. Here, we propose a spectropolarimeter enabled by…

Optics · Physics 2026-04-13 Jumin Qiu , Tingting Liu , Chenxuan Xiang , Tianbao Yu , Qiegen Liu , Shuyuan Xiao

Phase-only spatial light modulators (SLMs) are used in optical systems for several purposes. In this article, the main landmarks of SLM-based imaging systems are surveyed. In addition to conventional two-dimensional imaging, these systems…

Optics · Physics 2026-03-10 Joseph Rosen

We report on a high-resolution double-channel imaging method used to synchronously map the intensity- and optical-frequency-distribution of a laser beam in the plane orthogonal to the propagation direction. The synchronous measurement…

Optics · Physics 2015-06-08 Tao Wang , Gian-Luca Lippi

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…

Signal Processing · Electrical Eng. & Systems 2023-07-19 Thanh-An Pham , Emmanuel Soubies , Ferréol Soulez , Michael Unser

The Habitable Worlds Observatory requires active speckle suppression to directly image Earth-like exoplanets. Focal plane wavefront sensing and control allows us to detect, and subsequently remove, time-varying speckles through measurements…

Instrumentation and Methods for Astrophysics · Physics 2025-09-05 Joshua Liberman , Sebastiaan Y. Haffert , Jared R. Males , Kevin Derby , Ewan S. Douglas

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

Microscopies have become pillars of our characterization tools to observe biological systems and assemblies. Correlative and synchronous use of different microscopies relies on the fundamental assumption of non-interference during images…

The Fast Beam Condition Monitor (FBCM) is a standalone luminometer for the High Luminosity LHC (HL-LHC) program of the CMS Experiment at CERN. The detector is under development and features a new, radiation-hard, front-end…

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

Optics · Physics 2025-01-31 Oumeng Zhang , Changhuei Yang

The work describes the capabilities of Laser Scanning Microscopy (LSM) as a spatially resolved method of testing high_Tc materials and devices. The earlier results obtained by the authors are briefly reviewed. Some novel applications of the…

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Electromechanical coupling is ubiquitous in biological systems with examples ranging from simple piezoelectricity in calcified and connective tissues to voltage-gated ion channels, energy storage in mitochondria, and electromechanical…

Laser dynamics underpin a broad range of modern photonic technologies and continue to reveal rich nonlinear behaviors. However, existing spectroscopic tools, most notably time-stretched dispersive Fourier transform spectroscopy (TS-DFT),…

Scanning Kelvin probe microscopy (SKPM) is a powerful technique for investigating the electrostatic properties of material surfaces, enabling the imaging of variations in work function, topology, surface charge density, or combinations…

Applied Physics · Physics 2024-07-26 Isaac C. D. Lenton , Felix Pertl , Lubuna Shafeek , Scott R. Waitukaitis

A reliable extraction of filament data from microscopic images is of high interest in the analysis of acto-myosin structures as early morphological markers in mechanically guided differentiation of human mesenchymal stem cells and the…

Computer Vision and Pattern Recognition · Computer Science 2015-07-15 Benjamin Eltzner , Carina Wollnik , Carsten Gottschlich , Stephan Huckemann , Florian Rehfeldt

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…