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We present a new technique for monitoring microlensing activity even in highly crowded fields, and use this technique to place limits on low-mass MACHOs in the haloes of M31 and the Galaxy. Unlike present Galactic microlensing surveys, we…

Astrophysics · Physics 2009-09-28 Austin B. Tomaney , Arlin P. S. Crotts

Fluorescence fluctuations-based super-resolution microscopy (FF-SRM) is an emerging field promising low-cost and live-cell compatible imaging beyond the resolution of conventional optical microscopy. A comprehensive overview on how the…

Flow cytometry (FCM) is an analytic technique that is capable of detecting and recording the emission of fluorescence and light scattering of cells or particles (that are collectively called "events") in a population. A typical FCM…

Quantitative Methods · Quantitative Biology 2020-11-20 Nathan Wong , Daehwan Kim , Zachery Robinson , Connie Huang , Irina M. Conboy

Fibered confocal fluorescence microscopy (FCFM) is a new technique that can be used during a bronchoscopy to analyze the nature of the human bronchial and alveolar mucosa fluorescence microstructure. An endoscopic fibered confocal…

Medical Physics · Physics 2008-02-27 G. Bourg-Heckly , L. Thiberville , C. Vever-Bizet , B. Viellerobe

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…

Biological Physics · Physics 2014-03-13 Charles-Edouard Leroux , Alexei Grichine , Irène Wang , Antoine Delon

Light rays incident on a transparent object of uniform refractive index undergo deflections, which uniquely characterize the surface geometry of the object. Associated with each point on the surface is a deflection map (or spectrum) which…

Computer Vision and Pattern Recognition · Computer Science 2015-07-15 Prasad Sudhakar , Laurent Jacques , Xavier Dubois , Philippe Antoine , Luc Joannes

Speckle-correlation imaging techniques are widely used for non-invasive imaging through complex scattering media. While light propagation through multimode fibers and scattering media share many analogies, reconstructing images through…

Metasurfaces (MSs) have recently become an efficient solution for controlling a spatial field distribution according to the demand of an electromagnetic device. In particular, anomalous reflectors based on impenetrable MSs can be realized…

Applied Physics · Physics 2025-05-15 Vyacheslav Ivliev , Stanislav Glybovski

Objective: Survival rate of oral squamous cell carcinoma (OSCC) patients is very poor and can be improved using highly sensitive, specific and accurate techniques. Autofluorescence and fluorescence techniques are very sensitive and useful…

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Diffuse correlation spectroscopy (DCS) is an emerging noninvasive technique that measures the tissue blood flow, by using near-infrared coherent point-source illumination to detect spectral changes. While machine learning has demonstrated…

Machine Learning · Computer Science 2024-02-02 Xi Chen , Xingda Li

Transient Photoluminescence Microscopy (TPLM) allows for the direct visualization of carrier transport in semiconductor materials with sub nanosecond and few nanometer resolution. The technique is based on measuring changes in the spatial…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

The high-luminosity upgrade of the LHC brings unprecedented requirements for real-time and precision bunch-by-bunch online luminosity measurement and beam-induced background monitoring. A key component of the CMS Beam Radiation,…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Optical spectroscopy plays an essential role across scientific research and industry for non-contact materials analysis1-3, increasingly through in-situ or portable platforms4-6. However, when considering low-light-level applications,…

Photonic Lanterns (PLs) are tapered waveguides that gradually transition from a multi-mode fiber geometry to a bundle of single-mode fibers (SMFs). They can efficiently couple multi-mode telescope light into a multi-mode fiber entrance at…

Instrumentation and Methods for Astrophysics · Physics 2024-02-14 Yoo Jung Kim , Michael P. Fitzgerald , Jonathan Lin , Steph Sallum , Yinzi Xin , Nemanja Jovanovic , Sergio Leon-Saval

Imaging with a layered superlens is a spatial filtering operation characterized by the point spread function (PSF). We show that in the same optical system the image of a narrow sub-wavelength Gaussian incident field may be surprisingly…

Optics · Physics 2010-04-08 Rafal Kotynski , Tomasz Stefaniuk

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

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