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We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method…

Optics · Physics 2025-09-11 Charlie Kersuzan , Anirban Jana , Amaury Badon

The near-field diffraction of fs and sub-fs light pulses by nm-size slit-type apertures and its implication for near-field scanning optical microscopy (NSOM) is analyzed. The amplitude distributions of the diffracted wave-packets having the…

Optics · Physics 2013-10-11 S. V. Kukhlevsky , M. Mechler , L. Csapo

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. This manuscript reports the development of Polarized Localization Microscopy (PLM),…

Biological Physics · Physics 2017-03-31 Abir Maarouf Kabbani , Christopher V. Kelly

Computed tomography (CT) is widely used in scientific imaging systems such as synchrotron and laboratory-based nano-CT, but acquiring full-view sinograms requires high radiation dose and long scan times. Sparse-view CT reduces this burden…

Image and Video Processing · Electrical Eng. & Systems 2026-02-10 Jiaze E , Srutarshi Banerjee , Tekin Bicer , Guannan Wang , Yanfu Zhang , Bin Ren

Spatially entangled photon pairs (biphotons) generated by spontaneous parametric down-conversion offer unique opportunities for quantum imaging, but image-plane biphoton correlations are difficult to observe with camera-based detectors.…

Quantum Physics · Physics 2026-01-01 David McFadden , Rainer Heintzmann

The color X-ray camera (SLcam) is a full-field single photon imager. As stand-alone camera, it is applicable for energy and space-resolved X-ray detection measurements. The exchangeable poly-capillary optics in front of a beryllium entrance…

Instrumentation and Detectors · Physics 2015-06-11 A. Bjeoumikhov , G. Buzanich , N. Langhoff , I. Ordavo , M. Radtke , U. Reinholz , H. Riesemeier , O. Scharf , H. Soltau , R. Wedell

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

Optical micro-spectroscopy is an invaluable tool for studying and characterizing samples ranging from classical semiconductors to low-dimensional materials and heterostructures. To date, most implementations are based on point-scanning…

Image segmentation is a vital part of image processing. Segmentation has its application widespread in the field of medical images in order to diagnose curious diseases. The same medical images can be segmented manually. But the accuracy of…

Computer Vision and Pattern Recognition · Computer Science 2010-04-13 M. Gomathi , P. Thangaraj

Image deblurring aims to restore the detailed texture information or structures from blurry images, which has become an indispensable step in many computer vision tasks. Although various methods have been proposed to deal with the image…

Computer Vision and Pattern Recognition · Computer Science 2024-10-28 Yanni Zhang , Qiang Li , Miao Qi , Di Liu , Jun Kong , Jianzhong Wang

Accurate single-cell analysis is critical for diagnostics, immunomonitoring, and cell therapy, but coincident events - where multiple cells overlap in a sensing zone - can severely compromise signal fidelity. We present a hybrid framework…

Signal Processing · Electrical Eng. & Systems 2025-07-18 Moritz Leuthner , Rafael Vorländer , Oliver Hayden

Phase contrast microscopy (PCM) has been widely used in biomedicine research, which allows users to observe objectives without staining or killing them. One important related research is to employ PCM to monitor live cells. How to segment…

Computer Vision and Pattern Recognition · Computer Science 2019-11-26 Lin Zhang

The importance of spin-correlated radical pairs in biology is increasingly recognized, with roles in biological effects of weak magnetic fields and emerging quantum spin-based biomedical applications. Fluorescence microscopy offers…

Biological Physics · Physics 2025-10-08 Noboru Ikeya , Jonathan R. Woodward

Modeling the Point Spread Function (PSF) of wide-field surveys is vital for many astrophysical applications and cosmological probes including weak gravitational lensing. The PSF smears the image of any recorded object and therefore needs to…

Instrumentation and Methods for Astrophysics · Physics 2018-07-26 Jörg Herbel , Tomasz Kacprzak , Adam Amara , Alexandre Refregier , Aurelien Lucchi

Frequency-filtered photon correlations have been proven to be extremely useful in grasping how the detection process alters photon statistics. Harnessing the spectral correlations also permits refinement of the emission and unraveling of…

Lensless cameras offer significant advantages in size, weight, and cost compared to traditional lens-based systems. Without a focusing lens, lensless cameras rely on computational algorithms to recover the scenes from multiplexed…

Image and Video Processing · Electrical Eng. & Systems 2024-10-08 Xin Cai , Zhiyuan You , Hailong Zhang , Wentao Liu , Jinwei Gu , Tianfan Xue

Efficient ion-photon coupling is an important component for large-scale ion-trap quantum computing. We propose that arrays of phase Fresnel lenses (PFLs) are a favorable optical coupling technology to match with multi-zone ion traps. Both…

Quantum Physics · Physics 2011-01-25 E. W. Streed , B. G. Norton , J. J. Chapman , D. Kielpinski