Super-resolution Live-cell Fluorescence Lifetime Imaging
Optics
2025-02-25 v1
Abstract
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM makes it readily compatible with live-cell imaging. Here, we combine SR-SIM with electro-optic fluorescence lifetime imaging (EOFLIM), adding the capability of monitoring physicochemical parameters with 156 nm spatial resolution at high frame rate for live-cell imaging. We demonstrate that our new SIMFLIM technique enables super-resolved multiplexed imaging of spectrally overlapping fluorophores, environmental sensing, and live-cell imaging.
Cite
@article{arxiv.2502.16672,
title = {Super-resolution Live-cell Fluorescence Lifetime Imaging},
author = {Raphaël Marchand and Henning Ortkrass and Daniel Aziz and Franz Pfanner and Eman Abbas and Silvio O. Rizzoli and Wolfgang Hübner and Adam Bowman and Thomas Huser and Thomas Juffmann},
journal= {arXiv preprint arXiv:2502.16672},
year = {2025}
}