Sample Motion for Structured Illumination Fluorescence Microscopy
Optics
2025-06-17 v2 Image and Video Processing
Abstract
Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single speckle illumination pattern and relying on inherent sample motion to encode the super-resolved information in multiple raw images. From a set of raw fluorescence images captured as the sample moves, we jointly estimate both the sample motion and the super-resolved image. We demonstrate the feasibility of the proposed method both in simulation and in experiment.
Cite
@article{arxiv.2502.03667,
title = {Sample Motion for Structured Illumination Fluorescence Microscopy},
author = {Ruiming Cao and Guanghan Meng and Laura Waller},
journal= {arXiv preprint arXiv:2502.03667},
year = {2025}
}