Versatile luminescence macroscope with dynamic illumination for photoactive systems
Abstract
Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation, either modified commercial systems or fully bespoke setups, which poses a barrier for researchers without expertise in optics, electronics, or software. To address this, we present a versatile macroscopic fluorescence imaging system capable of supporting a wide range of protocols, and provide detailed build instructions along with open-source software to enable replication with minimal prior experience. We demonstrate its broad utility through applications to plants, reversibly photoswitchable fluorescent proteins, and optoelectronic devices.
Cite
@article{arxiv.2506.20270,
title = {Versatile luminescence macroscope with dynamic illumination for photoactive systems},
author = {Ian Coghill and Alienor Lahlou and Andrea Lodetti and Shizue Matsubara and Johann Boucle and Thomas Le Saux and Ludovic Jullien},
journal= {arXiv preprint arXiv:2506.20270},
year = {2025}
}
Comments
\c{opyright} 2025 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved. Link to paper (and supporting information): https://opg.optica.org/oe/abstract.cfm?doi=10.1364/OE.570450