Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution
Abstract
Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid progress is being made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. This review outlines developments in far-field fluorescence methods which enable ultrahigh resolution imaging and application of these techniques to biology. Future possible trends and directions in far-field fluorescence imaging with ultrahigh resolution are also outlined.
Cite
@article{arxiv.0708.3336,
title = {Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution},
author = {James H. Rice},
journal= {arXiv preprint arXiv:0708.3336},
year = {2007}
}
Comments
27 pages, 2 figures, 2 tables, review of devlopments in super resolution fluorescence microscopy