English

Optical Diffraction Tomography Meets Fluorescence Localization Microscopy

Signal Processing 2023-07-19 v1 Optics

Abstract

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the illumination sources are fluorophores within the sample. Building upon this model, we propose a joint optimization framework to estimate both the refractive index map and the position of fluorescent molecules from the sole SMLM frames.

Keywords

Cite

@article{arxiv.2307.09261,
  title  = {Optical Diffraction Tomography Meets Fluorescence Localization Microscopy},
  author = {Thanh-An Pham and Emmanuel Soubies and Ferréol Soulez and Michael Unser},
  journal= {arXiv preprint arXiv:2307.09261},
  year   = {2023}
}

Comments

Presented in ISCS23

R2 v1 2026-06-28T11:33:35.249Z