A common framework for single-molecule localization using sequential structured illumination
Abstract
Localization of single fluorescent molecules is key for physicochemical and biophysical measurements such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy (SMLM). Recently a series of methods have been developed in which the localization precision is enhanced by interrogating the molecular position with a sequence of spatially modulated patterns of light. Among them, the MINFLUX technique outstands for achieving a ~10-fold improvement compared to wide-field camera-based single-molecule localization, reaching ~1-2 nm localization precision at moderate photon counts. Here, we present a common mathematical framework for this type of measurement that allows a fair comparison between reported methods and facilitates the design and evaluation of new methods. With it, we benchmark all reported methods for single-molecule localization using sequential structured illumination, including long-established methods such as orbital tracking, along with two new proposed methods: orbital tracking and raster scanning with a minimum of intensity.
Cite
@article{arxiv.2109.01234,
title = {A common framework for single-molecule localization using sequential structured illumination},
author = {Luciano A. Masullo and Lucía F. Lopez and Fernando D. Stefani},
journal= {arXiv preprint arXiv:2109.01234},
year = {2021}
}