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Current all-atom potential based molecular dynamics (MD) allow the identification of a protein's functional motions on a wide-range of time-scales, up to few tens of ns. However, functional large scale motions of proteins may occur on a…

统计力学 · 物理学 2007-05-23 Cristian Micheletti , Paolo Carloni , Amos Maritan

3D Gaussians, as a low-level scene representation, typically involve thousands to millions of Gaussians. This makes it difficult to control the scene in ways that reflect the underlying dynamic structure, where the number of independent…

计算机视觉与模式识别 · 计算机科学 2024-12-24 Yunchao Zhang , Guandao Yang , Leonidas Guibas , Yanchao Yang

Advances in hyperspectral imaging modes including electron energy loss spectroscopy (EELS) in scanning transmission electron microscopy (STEM) bring forth the challenges of exploratory and subsequently physics-based analysis of…

计算物理 · 物理学 2020-05-22 Sergei V. Kalinin , Andrew R. Lupini , Rama K. Vasudevan , Maxim Ziatdinov

We address recovery of the three-dimensional backbone structure of single polypeptide proteins from single-particle cryo-electron microscopy (Cryo-SPA) data. Cryo-SPA produces noisy tomographic projections of electrostatic potentials of…

生物大分子 · 定量生物学 2024-10-02 Erik Jansson , Jonathan Krook , Klas Modin , Ozan Öktem

Energy dispersive X-ray (EDX) spectrum imaging yields compositional information with a spatial resolution down to the atomic level. However, experimental limitations often produce extremely sparse and noisy EDX spectra. Under such…

Principal Component Analysis (PCA), a classical dimensionality reduction technique, and 2D Gaussian representation, an adaptation of 3D Gaussian Splatting for image representation, offer distinct approaches to modeling visual data. We…

计算机视觉与模式识别 · 计算机科学 2025-03-13 Lo-Wei Tai , Ching-En Li , Cheng-Lin Chen , Chih-Jung Tsai , Hwann-Tzong Chen , Tyng-Luh Liu

Cellular Electron CryoTomography (CECT) is a 3D imaging technique that captures information about the structure and spatial organization of macromolecular complexes within single cells, in near-native state and at sub-molecular resolution.…

定量方法 · 定量生物学 2018-07-04 Kai Wen Wang , Xiangrui Zeng , Xiaodan Liang , Zhiguang Huo , Eric P. Xing , Min Xu

Understanding sub-cellular protein localisation is an essential component to analyse context specific protein function. Recent advances in quantitative mass-spectrometry (MS) have led to high resolution mapping of thousands of proteins to…

应用统计 · 统计学 2019-03-12 Oliver M. Crook , Kathryn S. Lilley , Laurent Gatto , Paul D. W. Kirk

The use of high-dimensional data for targeted therapeutic interventions requires new ways to characterize the heterogeneity observed across subgroups of a specific population. In particular, models for partially exchangeable data are needed…

统计方法学 · 统计学 2020-08-18 Francesco Denti , Federico Camerlenghi , Michele Guindani , Antonietta Mira

The field of cryo-electron microscopy has made astounding advancements in the past few years, mainly due to advancements in electron detectors' technology. Yet, one of the key open challenges of the field remains the processing of…

计算机视觉与模式识别 · 计算机科学 2019-10-04 Yariv Aizenbud , Yoel Shkolnisky

Cellular Electron Cryo-Tomography (CECT) is a powerful imaging technique for the 3D visualization of cellular structure and organization at submolecular resolution. It enables analyzing the native structures of macromolecular complexes and…

定量方法 · 定量生物学 2018-05-16 Chang Liu , Xiangrui Zeng , Ruogu Lin , Xiaodan Liang , Zachary Freyberg , Eric Xing , Min Xu

Current personalized neural head avatars face a trade-off: lightweight models lack detail and realism, while high-quality, animatable avatars require significant computational resources, making them unsuitable for commodity devices. To…

计算机视觉与模式识别 · 计算机科学 2025-04-01 Wojciech Zielonka , Timo Bolkart , Thabo Beeler , Justus Thies

Recent breakthroughs in high-resolution imaging of biomolecules in solution with cryo-electron microscopy (cryo-EM) have unlocked new doors for the reconstruction of molecular volumes, thereby promising further advances in biology,…

图像与视频处理 · 电气工程与系统科学 2022-05-27 Claire Donnat , Axel Levy , Frederic Poitevin , Ellen Zhong , Nina Miolane

We describe a novel coarse-grained simulation method for modelling the dynamics of globular macromolecules, such as proteins. The macromolecule is treated as a continuum that is subject to thermal fluctuations. The model includes a…

计算物理 · 物理学 2015-06-11 Robin Oliver , Daniel J. Read , Oliver G. Harlen , Sarah A. Harris

Scanning transmission electron microscopy (STEM) has become the technique of choice for quantitative characterization of atomic structure of materials, where the minute displacements of atomic columns from high-symmetry positions can be…

材料科学 · 物理学 2021-10-05 Kevin M. Roccapriore , Nicole Creange , Maxim Ziatdinov , Sergei V. Kalinin

The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this article, we show that the basic premise of cryo-EM --- patching together…

计算机视觉与模式识别 · 计算机科学 2017-04-25 Ke Ye , Lek-Heng Lim

We investigate the accuracy and efficiency of the semiclassical Frozen Gaussian method in describing electron dynamics in real time. Model systems of two soft-Coulomb-interacting electrons are used to study correlated dynamics under…

化学物理 · 物理学 2015-05-30 Peter Elliott , Neepa T. Maitra

Electrospray ion beam deposition (ESIBD) is the intact, chemically selective deposition of molecular ions on surfaces in vacuum. Here, we present a general method and dedicated instrumentation for ESIBD-based cryoEM sample preparation of…

Conformational sampling of biomolecules using molecular dynamics simulations often produces large amount of high dimensional data that makes it difficult to interpret using conventional analysis techniques. Dimensionality reduction methods…

生物大分子 · 定量生物学 2021-12-08 Mahdi Ghorbani , Samarjeet Prasad , Jeffery B. Klauda , Bernard R. Brooks

Over the past decade, cryogenic electron microscopy (cryo-EM) has emerged as a primary method for determining near-native, near-atomic resolution 3D structures of biological macromolecules. In order to meet increasing demand for cryo-EM,…

图像与视频处理 · 电气工程与系统科学 2022-01-19 Paul T. Kim , Alex J. Noble , Anchi Cheng , Tristan Bepler