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Designing enzymes with substrate-binding pockets is a critical challenge in protein engineering, as catalytic activity depends on the precise interaction between pockets and substrates. Currently, generative models dominate functional…

生物大分子 · 定量生物学 2026-01-28 Zefeng Lin , Zhihang Zhang , Weirong Zhu , Tongchang Han , Xianyong Fang , Tianfan Fu , Xiaohua Xu

Enzyme design is a critical area in biotechnology, with applications ranging from drug development to synthetic biology. Traditional methods for enzyme function prediction or protein binding pocket design often fall short in capturing the…

机器学习 · 计算机科学 2024-10-02 Chenqing Hua , Yong Liu , Dinghuai Zhang , Odin Zhang , Sitao Luan , Kevin K. Yang , Guy Wolf , Doina Precup , Shuangjia Zheng

Enzymes are nano-scale machines that have evolved to drive chemical reactions out of equilibrium in the right place at the right time. Given the complexity and specificity of enzymatic function, bottom-up design of enzymes presents a…

软凝聚态物质 · 物理学 2025-05-28 Michalis Chatzittofi , Jaime Agudo-Canalejo , Ramin Golestanian

Enzyme design plays a crucial role in both industrial production and biology. However, this field faces challenges due to the lack of comprehensive benchmarks and the complexity of enzyme design tasks, leading to a dearth of systematic…

生物大分子 · 定量生物学 2024-08-21 Jiangbin Zheng , Han Zhang , Qianqing Xu , An-Ping Zeng , Stan Z. Li

Enzymes are genetically encoded biocatalysts capable of accelerating chemical reactions. How can we automatically design functional enzymes? In this paper, we propose EnzyGen, an approach to learn a unified model to design enzymes across…

机器学习 · 计算机科学 2026-03-03 Zhenqiao Song , Yunlong Zhao , Wenxian Shi , Wengong Jin , Yang Yang , Lei Li

Sparked by innovations in generative artificial intelligence (AI), the field of protein design has undergone a paradigm shift with an explosion of new models for optimizing existing enzymes or creating them from scratch. After more than one…

生物大分子 · 定量生物学 2026-02-04 Lasse Middendorf , Noelia Ferruz

Designing enzyme backbones with substrate-specific functionality is a critical challenge in computational protein engineering. Current generative models excel in protein design but face limitations in binding data, substrate-specific…

生物大分子 · 定量生物学 2025-10-30 Chao Song , Zhiyuan Liu , Han Huang , Liang Wang , Qiong Wang , Jianyu Shi , Hui Yu , Yihang Zhou , Yang Zhang

Proteins are dynamic molecular machines whose biological functions, spanning enzymatic catalysis, signal transduction, and structural adaptation, are intrinsically linked to their motions. Designing proteins with targeted dynamic…

生物大分子 · 定量生物学 2025-02-17 Bo Ni , Markus J. Buehler

We present SeedProteo, a diffusion-based model for de novo all-atom protein design. We demonstrate how to repurpose a cutting-edge folding architecture into a powerful generative design framework by effectively integrating self-conditioning…

生物大分子 · 定量生物学 2026-02-25 Wei Qu , Yiming Ma , Fei Ye , Chan Lu , Yi Zhou , Kexin Zhang , Lan Wang , Minrui Gui , Quanquan Gu

Spatial organization in metabolic pathways can arise from the interplay between enzymatic reaction kinetics and diffusion-driven instabilities. In this work we investigate how reversible enzyme--substrate binding influences pattern…

分子网络 · 定量生物学 2026-04-27 Faezeh Farivar

Enzymes, with their specific catalyzed reactions, are necessary for all aspects of life, enabling diverse biological processes and adaptations. Predicting enzyme functions is essential for understanding biological pathways, guiding drug…

机器学习 · 计算机科学 2024-10-02 Chenqing Hua , Bozitao Zhong , Sitao Luan , Liang Hong , Guy Wolf , Doina Precup , Shuangjia Zheng

Structure-based drug design involves finding ligand molecules that exhibit structural and chemical complementarity to protein pockets. Deep generative methods have shown promise in proposing novel molecules from scratch (de-novo design),…

定量方法 · 定量生物学 2021-11-09 Pavol Drotár , Arian Rokkum Jamasb , Ben Day , Cătălina Cangea , Pietro Liò

Understanding and modeling enzyme-substrate interactions is crucial for catalytic mechanism research, enzyme engineering, and metabolic engineering. Although a large number of predictive methods have emerged, they do not incorporate prior…

生物大分子 · 定量生物学 2025-06-24 Zhiwei Nie , Hongyu Zhang , Hao Jiang , Yutian Liu , Xiansong Huang , Fan Xu , Jie Fu , Zhixiang Ren , Yonghong Tian , Wen-Bin Zhang , Jie Chen

De novo molecular design has facilitated the exploration of large chemical space to accelerate drug discovery. Structure-based de novo method can overcome the data scarcity of active ligands by incorporating drug-target interaction into…

生物大分子 · 定量生物学 2022-09-16 Yaqin Li , Lingli Li , Yongjin Xu , Yi Yu

Deep learning has transformed protein design, enabling accurate structure prediction, sequence optimization, and de novo protein generation. Advances in single-chain protein structure prediction via AlphaFold2, RoseTTAFold, ESMFold, and…

机器学习 · 计算机科学 2025-02-27 Gregory W. Kyro , Tianyin Qiu , Victor S. Batista

Evolution is an extraordinary engine for enzymatic diversity, yet the chemistry it has explored remains a narrow slice of what DNA can encode. Deep generative models can design new proteins that bind ligands, but none have created enzymes…

The de novo design of proteins refers to creating proteins with specific structures and functions that do not naturally exist. In recent years, the accumulation of high-quality protein structure and sequence data and technological…

生物大分子 · 定量生物学 2025-04-24 Yujie Qin , Ming He , Changyong Yu , Ming Ni , Xian Liu , Xiaochen Bo

In this paper we present a new method for determining optimal designs for enzyme inhibition kinetic models, which are used to model the influence of the concentration of a substrate and an inhibition on the velocity of a reaction. The…

统计方法学 · 统计学 2017-09-18 Kirsten Schorning , Holger Dette , Katrin Kettelhake , Tilman Möller

Function-guided protein design is a crucial task with significant applications in drug discovery and enzyme engineering. However, the field lacks a unified and comprehensive evaluation framework. Current models are assessed using…

机器学习 · 计算机科学 2025-09-30 Jiahao Kuang , Nuowei Liu , Jie Wang , Changzhi Sun , Tao Ji , Yuanbin Wu

The design of \emph{de novo} functional proteins that bind specific ligand molecules is paramount in therapeutics and bio-engineering. A critical yet formidable task in this endeavor is the design of the protein pocket, which is the cavity…

生物大分子 · 定量生物学 2023-10-23 Zaixi Zhang , Zepu Lu , Zhongkai Hao , Marinka Zitnik , Qi Liu
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