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We propose a general framework for a collaborative machine learning system to assist bioscience researchers with the task of labeling specific cell identities from microscopic still or video imaging. The distinguishing features of this…

定量方法 · 定量生物学 2019-03-25 Greg Bubnis , Steven Ban , Matthew D. DiFranco , Saul Kato

Cell detection is the task of detecting the approximate positions of cell centroids from microscopy images. Recently, convolutional neural network-based approaches have achieved promising performance. However, these methods require a…

计算机视觉与模式识别 · 计算机科学 2021-07-20 Kazuya Nishimura , Hyeonwoo Cho , Ryoma Bise

Determining the trajectories of cells and their lineages or ancestries in live-cell experiments are fundamental to the understanding of how cells behave and divide. This paper proposes novel online algorithms for jointly tracking and…

信号处理 · 电气工程与系统科学 2021-10-29 Tran Thien Dat Nguyen , Ba-Ngu Vo , Ba-Tuong Vo , Du Yong Kim , Yu Suk Choi

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

图像与视频处理 · 电气工程与系统科学 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Tracking cells in time-lapse videos is an essential technique for monitoring cell population dynamics at a single-cell level. Current methods for cell tracking are developed on videos with mostly single, constant signals and do not detect…

计算机视觉与模式识别 · 计算机科学 2025-12-17 Florian Bürger , Martim Dias Gomes , Nica Gutu , Adrián E. Granada , Noémie Moreau , Katarzyna Bozek

Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…

计算机视觉与模式识别 · 计算机科学 2017-05-10 Saad Ullah Akram , Juho Kannala , Lauri Eklund , Janne Heikkilä

Label-free cell classification is advantageous for supplying pristine cells for further use or examination, yet existing techniques frequently fall short in terms of specificity and speed. In this study, we address these limitations through…

图像与视频处理 · 电气工程与系统科学 2025-02-25 Khayrul Islam , Ratul Paul , Shen Wang , Yuwen Zhao , Partho Adhikary , Qiying Li , Xiaochen Qin , Yaling Liu

Cell tracking is a key computational task in live-cell microscopy, but fully automated analysis of high-throughput imaging requires reliable and, thus, uncertainty-aware data analysis tools, as the amount of data recorded within a single…

计算机视觉与模式识别 · 计算机科学 2025-03-13 Richard D. Paul , Johannes Seiffarth , David Rügamer , Hanno Scharr , Katharina Nöh

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

3D cell tracking in a living organism has a crucial role in live cell image analysis. Cell tracking in C. elegans has two difficulties. First, cell migration in a consecutive frame is large since they move their head during scanning.…

计算机视觉与模式识别 · 计算机科学 2024-03-21 Kaito Shiku , Hiromitsu Shirai , Takeshi Ishihara , Ryoma Bise

The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object tracking methods. In recent years, several existing and new applications have…

图像与视频处理 · 电气工程与系统科学 2025-02-03 Gergely Szabó , Paolo Bonaiuti , Andrea Ciliberto , András Horváth

A novel and fast semi-automatic method for segmentation, locating and counting blood cells in an image is proposed. In this method, thresholding is used to separate the nucleus from the other parts. We also use Hough transform for circles…

图像与视频处理 · 电气工程与系统科学 2020-04-21 Hamed Sadeghi , Shahram Shirani , David W. Capson

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

计算机视觉与模式识别 · 计算机科学 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

计算机视觉与模式识别 · 计算机科学 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Fluorescence labeling is the standard approach to reveal cellular structures and other subcellular constituents for microscopy images. However, this invasive procedure may perturb or even kill the cells and the procedure itself is highly…

图像与视频处理 · 电气工程与系统科学 2024-06-25 Han Liu , Hao Li , Jiacheng Wang , Yubo Fan , Zhoubing Xu , Ipek Oguz

Quantitative analysis of the dynamics of tiny cellular and sub-cellular structures, known as particles, in time-lapse cell microscopy sequences requires the development of a reliable multi-target tracking method capable of tracking numerous…

计算机视觉与模式识别 · 计算机科学 2015-07-24 Seyed Hamid Rezatofighi , Stephen Gould , Ba Tuong Vo , Ba-Ngu Vo , Katarina Mele , Richard Hartley

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

计算机视觉与模式识别 · 计算机科学 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

Cell image classification methods are currently being used in numerous applications in cell biology and medicine. Applications include understanding the effects of genes and drugs in screening experiments, understanding the role and…

定量方法 · 定量生物学 2022-03-04 Mohammad Shifat-E-Rabbi , Xuwang Yin , Cailey Elizabeth Fitzgerald , Gustavo K. Rohde

Cell detection is an essential task in cell image analysis. Recent deep learning-based detection methods have achieved very promising results. In general, these methods require exhaustively annotating the cells in an entire image. If some…

计算机视觉与模式识别 · 计算机科学 2021-07-22 Kazuma Fujii , Daiki Suehiro , Kazuya Nishimura , Ryoma Bise

Cell tracking is an essential tool in live-cell imaging to determine single-cell features, such as division patterns or elongation rates. Unlike in common multiple object tracking, in microbial live-cell experiments cells are growing,…

计算机视觉与模式识别 · 计算机科学 2023-03-20 Karina Ruzaeva , Jan-Christopher Cohrs , Keitaro Kasahara , Dietrich Kohlheyer , Katharina Nöh , Benjamin Berkels
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