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Intrinsically disordered proteins (IDPs) are typically low in nonpolar/hydrophobic but relatively high in polar, charged, and aromatic amino acid compositions. Some IDPs undergo liquid-liquid phase separation in the aqueous milieu of the…

生物大分子 · 定量生物学 2017-05-19 Yi-Hsuan Lin , Jianhui Song , Julie D. Forman-Kay , Hue Sun Chan

Endeavoring toward a transferable, predictive coarse-grained explicit-chain model for biomolecular condensates underlain by liquid-liquid phase separation (LLPS), we conducted multiple-chain simulations of the N-terminal intrinsically…

生物大分子 · 定量生物学 2020-12-10 Suman Das , Yi-Hsuan Lin , Robert M. Vernon , Julie D. Forman-Kay , Hue Sun Chan

Biomolecular condensates, physically underpinned to a significant extent by liquid-liquid phase separation (LLPS), are now widely recognized by numerous experimental studies to be of fundamental biological, biomedical, and biophysical…

生物大分子 · 定量生物学 2022-10-18 Yi-Hsuan Lin , Jonas Wessén , Tanmoy Pal , Suman Das , Hue Sun Chan

Biomolecular condensates undergirded by phase separations of proteins and nucleic acids serve crucial biological functions. To gain physical insights into their genetic basis, we study how liquid-liquid phase separation (LLPS) of…

生物大分子 · 定量生物学 2018-12-03 Suman Das , Alan Amin , Yi-Hsuan Lin , Hue Sun Chan

Biologically functional liquid-liquid phase separation of intrinsically disordered proteins (IDPs) is driven by interactions encoded by their amino acid sequences. Little is currently known about the molecular recognition mechanisms for…

生物大分子 · 定量生物学 2017-11-15 Yi-Hsuan Lin , Jacob P. Brady , Julie D. Forman-Kay , Hue Sun Chan

In view of recent intense experimental and theoretical interests in the biophysics of liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs), heteropolymer models with chain molecules configured as self-avoiding…

生物大分子 · 定量生物学 2018-08-30 Suman Das , Adam Eisen , Yi-Hsuan Lin , Hue Sun Chan

The physical chemistry of liquid-liquid phase separation (LLPS) of polymer solutions bears directly on the assembly of biologically functional droplet-like bodies from proteins and nucleic acids. These biomolecular condensates include…

生物大分子 · 定量生物学 2020-01-31 Yi-Hsuan Lin , Jacob P. Brady , Hue Sun Chan , Kingshuk Ghosh

We report simulation studies of 33 single intrinsically disordered proteins (IDPs) using coarse-grained (CG) bead-spring models where interactions among different amino acids are introduced through a hydropathy matrix and additional…

软凝聚态物质 · 物理学 2023-12-07 Swarnadeep Seth , Brandon Stine , Aniket Bhattacharya

Liquid-liquid phase separation (LLPS) of protein solutions is governed by highly complex protein-protein interactions. Nevertheless, it has been suggested that based on the extended law of corresponding states (ELCS), as proposed for…

软凝聚态物质 · 物理学 2023-02-08 Jan Hansen , Stefan U. Egelhaaf , Florian Platten

Biomolecular condensates such as membraneless organelles, underpinned by liquid-liquid phase separation (LLPS), are important for physiological function, with electrostatics -- among other interaction types -- being a prominent force in…

生物大分子 · 定量生物学 2021-05-26 Jonas Wessén , Tanmoy Pal , Suman Das , Yi-Hsuan Lin , Hue Sun Chan

The assembly of functional biomolecular condensates often involves liquid-liquid phase separation (LLPS) of proteins with multiple modular domains, which can be folded or conformationally disordered to various degrees. To understand the…

生物大分子 · 定量生物学 2022-01-11 Yi-Hsuan Lin , Haowei Wu , Bowen Jia , Mingjie Zhang , Hue Sun Chan

Liquid liquid phase separation (LLPS) of proteins is an intracellular process that is widely used by cells for many purposes. In living cells (in vivo), LLPS occurs in complex and crowded environments. Amino acids (AAs) are vital components…

We propose a simplified version of local molecular field (LMF) theory to treat Coulomb interactions in simulations of ionic fluids. LMF theory relies on splitting the Coulomb potential into a short-ranged part that combines with other…

统计力学 · 物理学 2009-11-13 Natalia A. Denesyuk , John D. Weeks

We investigate liquid-liquid phase separation (LLPS) and interfacial properties of two LLPS modes: associative (ALLPS) and segregative (SLLPS). Analytical expressions for the critical point (CP) and binodal boundaries are derived and show…

软凝聚态物质 · 物理学 2025-10-30 Remco Tuinier , Alvaro Gonzalez Garcia

Liquid-liquid phase separation of charge/aromatic-enriched intrinsically disordered proteins (IDPs) is critical in the biological function of membraneless organelles. Much of the physics of this recent discovery remains to be elucidated.…

生物大分子 · 定量生物学 2016-10-18 Yi-Hsuan Lin , Julie D. Forman-Kay , Hue Sun Chan

Synthetic copolymers and biopolymers, such as polypeptides and double-stranded DNA, often exhibit strong variations in bending stiffness along their contour, which can significantly impact conformational behavior at larger scales. To…

软凝聚态物质 · 物理学 2025-11-04 Yannick Witzky , Friederike Schmid , Arash Nikoubashman

Proteins can form droplets via liquid-liquid phase separation (LLPS) in cells. Recent experiments demonstrate that LLPS is qualitatively different on two-dimensional (2d) surfaces compared to three-dimensional (3d) solutions. In this paper,…

定量方法 · 定量生物学 2024-08-29 Jinyoung Kim , Sean D. Lawley , Jinsu Kim

Liquid-liquid phase separation of intrinsically disordered proteins (IDPs) is a major undergirding factor in the regulated formation of membraneless organelles in the cell. The phase behavior of an IDP is sensitive to its amino acid…

生物大分子 · 定量生物学 2022-01-11 Yi-Hsuan Lin , Hue Sun Chan

Liquid-liquid phase separation (LLPS) involving intrinsically disordered protein regions (IDRs) is a major physical mechanism for biological membraneless compartmentalization. The multifaceted electrostatic effects in these biomolecular…

The theory of first order density-driven phase transitions with frustration due to the long range Coulomb (LRC) interaction develop on paper I of this series is applied to the following physical systems: i) the low density electron gas ii)…

强关联电子 · 物理学 2016-08-31 J. Lorenzana , C. Castellani , C. Di Castro
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