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In single-particle cryo-electron microscopy (cryo-EM), the efficient determination of orientation parameters for 2D projection images poses a significant challenge yet is crucial for reconstructing 3D structures. This task is complicated by…

图像与视频处理 · 电气工程与系统科学 2025-09-30 Szu-Chi Chung

Electrical Impedance Tomography (EIT) is a powerful imaging technique with diverse applications, e.g., medical diagnosis, industrial monitoring, and environmental studies. The EIT inverse problem is about inferring the internal conductivity…

机器学习 · 计算机科学 2023-10-31 Derick Nganyu Tanyu , Jianfeng Ning , Andreas Hauptmann , Bangti Jin , Peter Maass

Particle picking is a time-consuming step in single-particle analysis and often requires significant interventions from users, which has become a bottleneck for future automated electron cryo-microscopy (cryo-EM). Here we report a deep…

定量方法 · 定量生物学 2016-05-09 Feng Wang , Huichao Gong , Gaochao liu , Meijing Li , Chuangye Yan , Tian Xia , Xueming Li , Jianyang Zeng

Differentiating signals from the background in micrographs is a critical initial step for cryogenic electron microscopy (cryo-EM), yet it remains laborious due to low signal-to-noise ratio (SNR), the presence of contaminants and densely…

图像与视频处理 · 电气工程与系统科学 2025-09-30 Szu-Chi Chung , Po-Cheng Chou

We revisit the topic of common lines between projection images in single particle cryo-electron microscopy (cryo-EM). We derive a novel low-rank constraint on a certain $2n \times n$ matrix storing properly-scaled basis vectors for the…

最优化与控制 · 数学 2024-06-10 Tommi Muller , Adriana L. Duncan , Eric J. Verbeke , Joe Kileel

Cryo-EM is a powerful tool for understanding macromolecular structures, yet current methods for structure reconstruction are slow and computationally demanding. To accelerate research on pose estimation, we present CESPED, a new dataset…

定量方法 · 定量生物学 2024-05-03 Ruben Sanchez-Garcia , Michael Saur , Javier Vargas , Carl Poelking , Charlotte M Deane

To study the fundamental physics of complex multiphase flow systems using advanced measurement techniques, especially the electrical capacitance tomography (ECT) approach, this article carries out an initial literature review of the ECT…

计算机视觉与模式识别 · 计算机科学 2015-10-16 Kezhi Li

The aim of this paper is to give an introductory review of the cryoelectron microscopy as a complex data source for the most of the system biology branches, including the most perspective non-local approaches known as "localomics" and…

亚细胞过程 · 定量生物学 2015-01-20 Oleg V. Gradov , Margaret A. Gradova

Laser flash melting and revitrification experiments have recently improved the time resolution of cryo-electron microscopy (cryo-EM) to the microsecond timescale, making it fast enough to observe many of the protein motions that are…

A major challenge in single-particle cryo-electron microscopy (cryo-EM) is that the orientations adopted by the 3D particles prior to imaging are unknown; yet, this knowledge is essential for high-resolution reconstruction. We present a…

机器学习 · 计算机科学 2021-04-14 Jelena Banjac , Laurène Donati , Michaël Defferrard

Soot is an important material with impacts that depend on particle morphology. Transmission electron microscopy (TEM) represents one of the most direct routes to qualitatively assess particle characteristics. However, producing quantitative…

应用物理 · 物理学 2022-02-01 Timothy A. Sipkens , Max Frei , Alberto Baldelli , P. Kirchen , Frank E. Kruis , Steven N. Rogak

We introduce a framework for recovering an image from its rotationally and translationally invariant features based on autocorrelation analysis. This work is an instance of the multi-target detection statistical model, which is mainly used…

图像与视频处理 · 电气工程与系统科学 2022-03-03 Nicholas F. Marshall , Ti-Yen Lan , Tamir Bendory , Amit Singer

Electron microscopy has enabled many scientific breakthroughs across multiple fields. A key challenge is the tuning of microscope parameters based on images to overcome optical aberrations that deteriorate image quality. This calibration…

计算机视觉与模式识别 · 计算机科学 2026-03-20 Jilles S. van Hulst , W. P. M. H. Heemels , Duarte J. Antunes

Advances on cryo-electron imaging technologies have led to a rapidly increasing number of density maps. Alignment and comparison of density maps play a crucial role in interpreting structural information, such as conformational…

计算机视觉与模式识别 · 计算机科学 2023-09-19 Bintao He , Fa Zhang , Chenjie Feng , Jianyi Yang , Xin Gao , Renmin Han

In cryo-electron microscopy (cryo-EM), a microscope generates a top view of a sample of randomly-oriented copies of a molecule. The problem of single particle reconstruction (SPR) from cryo-EM is to use the resulting set of noisy 2D…

数值分析 · 数学 2014-09-16 Gene Katsevich , Alexander Katsevich , Amit Singer

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a…

定量方法 · 定量生物学 2024-03-14 Jeremy J. Axelrod , Jessie T. Zhang , Petar N. Petrov , Robert M. Glaeser , Holger Mueller

Advances in imagery at atomic and near-atomic resolution, such as cryogenic electron microscopy (cryo-EM), have led to an influx of high resolution images of proteins and other macromolecular structures to data banks worldwide. Producing a…

定量方法 · 定量生物学 2021-04-06 Nathan Ranno , Dong Si

Cryo Focused Ion-Beam Scanning Electron Microscopy (cryo FIB-SEM) enables three-dimensional and nanoscale imaging of biological specimens via a slice and view mechanism. The FIB-SEM experiments are, however, limited by a slow (typically,…

Scanning transmission electron microscopy (STEM) is an extremely versatile method for studying materials on the atomic scale. Many STEM experiments are supported or validated with electron scattering simulations. However, using the…

The shape of a molecule determines its physicochemical and biological properties. However, it is often underrepresented in standard molecular representation learning approaches. Here, we propose using the Euler Characteristic Transform…

机器学习 · 计算机科学 2025-07-08 Victor Toscano-Duran , Florian Rottach , Bastian Rieck