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Double-stranded DNA translocates through sufficiently large nanopores either in a linear, single-file fashion or in a folded hairpin conformation when captured somewhere along its length. We show that the folding state of DNA can be…

生物物理 · 物理学 2018-08-15 Niklas Ermann , Nikita Hanikel , Vivian Wang , Kaikai Chen , Ulrich F. Keyser

A solid-state nanopore can electrophoretically capture a DNA molecule and pull it through in a folded configuration. The resulting ionic current signal indicates where along its length the DNA was captured. A statistical study using an 8 nm…

生物物理 · 物理学 2015-06-11 Mirna Mihovilovic , Nick Hagerty , Derek Stein

The translocation of a macromolecule through a nanometer-sized pore is an interesting process with important applications in the development of biosensors for single--molecule analysis and in drug delivery and gene therapy. We have carried…

统计力学 · 物理学 2009-09-29 Yves Lansac , Prabal K. Maiti , Matthew A. Glaser

We study voltage driven translocation of a single stranded (ss) DNA through a membrane channel. Our model, based on a master equation (ME) approach, investigates the probability density function (pdf) of the translocation times, and shows…

统计力学 · 物理学 2007-05-23 O. Flomenbom , J. Klafter

We investigate the dynamics of DNA translocation through a nanopore driven by an external force using Langevin dynamics simulations in two dimensions (2D) to study how the translocation dynamics depend on the details of the DNA sequences.…

软凝聚态物质 · 物理学 2008-12-09 Kaifu Luo , Tapio Ala-Nissila , See-Chen Ying , Aniket Bhattacharya

We discuss multiscale simulations of long biopolymer translocation through wide nanopores that can accommodate multiple polymer strands. The simulations provide clear evidence of folding quantization, namely, the translocation proceeds…

生物物理 · 物理学 2009-11-13 Simone Melchionna , Massimo Bernaschi , Maria Fyta , Efthimios Kaxiras , Sauro Succi

Graphene nanopore based sensor devices are exhibiting the great potential for the detection of DNA. To understand the fundamental aspects of DNA translocating through a graphene nanopore, in this work, molecular dynamics (MD) simulations…

生物物理 · 物理学 2012-11-30 Wenping Lv , Maodu Chen , Renan Wu

Polymer translocation through a nanopore in a membrane investigated theoretically. Recent experiments on voltage-driven DNA and RNA translocations through a nanopore indicate that the size and geometry of the pore are important factors in…

统计力学 · 物理学 2009-11-07 Elena Slonkina , Anatoly B. Kolomeisky

DNA translocation through nanopores is one of the most promising strategies for the next-generation sequencing technologies. Most part of experimental and numerical works has focused on polymer translocation biased by electrophoresis, where…

软凝聚态物质 · 物理学 2018-02-07 Timothée Menais

We consider the influence of electrostatic forces on driven translocation dynamics of a flexible polyelectrolyte being pulled through a nanopore by an external force on the head monomer. To this end, we augment the iso-flux tension…

软凝聚态物质 · 物理学 2020-07-07 Jalal Sarabadani , Sahin Buyukdagli , Tapio Ala-Nissila

The threading of a polymer chain through a small pore is a classic problem in polymer dynamics and underlies nanopore sensing technology. However important experimental aspects of the polymer motion in a solid-state nanopore, such as an…

生物物理 · 物理学 2016-07-18 Nicholas A. W. Bell , Ulrich F. Keyser

We investigate the dynamics of DNA translocation through a nanopore using 2D Langevin dynamics simulations, focusing on the dependence of the translocation dynamics on the details of DNA sequences. The DNA molecules studied in this work are…

软凝聚态物质 · 物理学 2008-02-05 Kaifu Luo , Tapio Ala-Nissila , See-Chen Ying , Aniket Bhattacharya

Polymer translocation is a promising strategy for the next-generation DNA sequencing technologies. The use of biological and synthetic nano-pores, however, still suffers from serious drawbacks. In particular, the width of the membrane layer…

软凝聚态物质 · 物理学 2017-11-30 Timothee Menais , Stefano Mossa , Arnaud Buhot

We investigate the translocation of a single stranded DNA through a pore which fluctuates between two conformations, using coupled master equations. The probability density function of the first passage times (FPT) of the translocation…

统计力学 · 物理学 2007-05-23 O. Flomenbom , J. Klafter

The detection of biomolecules at the single molecular level have important applications in the fields of biosensing and biomedical diagnosis. Solid state nanopore (SS-nanopore) is an effective tool to perform the single molecular detection,…

生物物理 · 物理学 2022-10-14 Jing Yang , Juan Wang , Ranfeng Wu , Yiming Chen , Cheng Zhang

Nanopores -- nanosized holes that can transport ions and molecules -- are very promising devices for genomic screening, in particular DNA sequencing. Both solid-state and biological pores suffer from the drawback, however, that the channel…

Translocation of a single stranded DNA through genetically engineered $\alpha$-hemolysin channels with positively charged walls is studied. It is predicted that transport properties of such channels are dramatically different from neutral…

亚细胞过程 · 定量生物学 2008-09-10 Tao Hu , B. I. Shklovskii

A multiscale approach is used to simulate the translocation of DNA through a nanopore. Within this scheme, the interactions of the molecule with the surrounding fluid (solvent) are explicitly taken into account. By generating polymers of…

生物物理 · 物理学 2009-11-13 Simone Melchionna , Maria Fyta , Efthimios Kaxiras , Sauro Succi

The passage of DNA through a nanopore can be effectively decomposed into two distinct phases, docking and actual translocation. In experiments each phase is characterized by a distinct current signature which allows the discrimination of…

In this short note, a correction is made to the recently proposed solution [1] to a 1D biased diffusion model for linear DNA translocation and a new analysis will be given to the data in [1]. It was pointed out [2] by us recently that this…

软凝聚态物质 · 物理学 2013-08-23 Daniel Y. Ling , X. S. Ling
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