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The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. This manuscript reports the development of Polarized Localization Microscopy (PLM),…

生物物理 · 物理学 2017-03-31 Abir Maarouf Kabbani , Christopher V. Kelly

Evanescent light excitation is widely used in super-resolution fluorescence microscopy to confine light and reduce background noise. Herein we propose a method of exploiting evanescent light in the context of emission. When a fluorophore is…

生物物理 · 物理学 2015-09-30 N. Bourg , C. Mayet , G. Dupuis , T. Barroca , P. Bon , S. Lécart , E. Fort , S. Lévêque-Fort

This paper studies a near-field multiple-input multiple-output (MIMO) radar sensing system, in which the transceivers with massive antennas aim to localize multiple near-field targets in the three-dimensional (3D) space over unknown…

信息论 · 计算机科学 2023-08-31 Haocheng Hua , Jie Xu , Yonina C. Eldar

We report a photoluminescence imaging system for locating single quantum emitters with respect to alignment features. Samples are interrogated in a 4~K closed-cycle cryostat by a high numerical aperture (NA=0.9, 100$\times$ magnification)…

The localisation of fluorophores is an important aspect of the determination of the biological function of cellular systems. Quantum correlation microscopy (QCM) is a promising technique for providing diffraction unlimited emitter…

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

光学 · 物理学 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

The detection of landmarks or patterns is of interest for extracting features in biological images. Hence, algorithms for finding these keypoints have been extensively investigated in the literature, and their localization and detection…

信息论 · 计算机科学 2017-10-11 Zsuzsanna Püspöki , Arash Amini , Julien Fageot , John Paul Ward , Michael Unser

The orientation of luminescent molecules in organic light-emitting diodes (OLEDs) strongly influences device performance. However, our understanding of the factors controlling emitter orientation is limited as current measurements only…

Emitter localization is widely applied in the military and civilian _elds. In this paper, we tackle the problem of position estimation for multiple stationary emitters using Doppler frequency shifts and angles by moving receivers. The…

信号处理 · 电气工程与系统科学 2021-12-07 Ziqiang Wang , Yimao Sun , Qun Wan , Lei Xie , Ning Liu

Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…

The precise determination of the position of point-like emitters and scatterers using far-field optical imaging techniques is of utmost importance for a wide range of applications in medicine, biology, astronomy, and physics. Although the…

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…

光学 · 物理学 2020-08-05 Daniel Böning , Franz-Ferdinand Wieser , Vahid Sandoghdar

We investigate a wireless network localization scenario in which the need for synchronized nodes is avoided. It consists of a set of fixed anchor nodes transmitting according to a given sequence and a self-localizing receiver node. The…

统计理论 · 数学 2013-04-24 Dave Zachariah , Alessio De Angelis , Satyam Dwivedi , Peter Händel

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

光学 · 物理学 2024-03-26 Tingting Wu , Matthew D. Lew

Interferometric imaging is an emerging technique for particle tracking and mass photometry. Mass or position are estimated from weak signals, coherently scattered from nanoparticles or single molecules, and interfered with a co-propagating…

光学 · 物理学 2021-08-11 Jonathan Dong , Dante Maestre , Clara Conrad-Billroth , Thomas Juffmann

Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…

The localization of directional RF emitters presents significant challenges for electronic warfare applications. Traditional localization methods, designed for omnidirectional emitters, experience degraded performance when applied to…

信号处理 · 电气工程与系统科学 2025-10-30 Fraser Williams , Akila Pemasiri , Dhammika Jayalath , Terry Martin , Clinton Fookes

Future sixth-generation (6G) networks are envisioned to provide both sensing and communications functionalities by using densely deployed base stations (BSs) with massive antennas operating in millimeter wave (mmWave) and terahertz (THz).…

信息论 · 计算机科学 2023-08-16 Haocheng Hua , Jie Xu

Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…

生物物理 · 物理学 2017-05-08 Martin Lindén , Vladimir Ćurić , Elias Amselem , Johan Elf

We report an experimental technique to map and exploit the local density of optical states of arbitrary planar nano-photonic structures. The method relies on positioning a spontaneous emitter attached to a scanning probe deterministically…

光学 · 物理学 2012-08-22 Martin Frimmer , Yuntian Chen , A. Femius Koenderink