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CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers.…

种群与进化 · 定量生物学 2017-11-28 Melia E. Bonomo , Michael W. Deem

Bacteria and archaea have evolved an adaptive, heritable immune system that recognizes and protects against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or…

种群与进化 · 定量生物学 2014-01-21 Pu Han , Liang Ren Niestemski , Jeffrey E. Barrick , Michael W. Deem

CRISPR-Cas systems are an adaptive immunity that protects prokaryotes against foreign genetic elements. Genetic templates acquired during past infection events enable DNA-interacting enzymes to recognize foreign DNA for destruction. Due to…

基因组学 · 定量生物学 2022-02-16 Hyunjin Shim

Features of the CRISPR-Cas system, in which bacteria integrate small segments of phage genome (spacers) into their DNA to neutralize future attacks, suggest that its effect is not limited to individual bacteria but may control the fate and…

种群与进化 · 定量生物学 2018-07-30 Madeleine Bonsma-Fisher , Dominique Soutiere , Sidhartha Goyal

CRISPR-Cas mediated immunity in bacteria allows bacterial populations to protect themselves against pathogens. However, it also exposes them to the dangers of auto-immunity by developing protection that targets its own genome. Using a…

种群与进化 · 定量生物学 2018-11-12 Serena Bradde , Thierry Mora , Aleksandra M. Walczak

Today, the CRISPR (clustered regularly interspaced short palindromic repeats) region within bacterial and archaeal genomes is known to encode an adaptive immune system. We rely on previous results on the evolution of the CRISPR arrays,…

概率论 · 数学 2017-09-19 Franz Baumdicker , Ariana M. I. Huebner , Peter Pfaffelhuber

The CRISPR (clustered regularly interspaced short palindromic repeats) mechanism allows bacteria to adaptively defend against phages by acquiring short genomic sequences (spacers) that target specific sequences in the viral genome. We…

生物物理 · 物理学 2019-09-11 Serena Bradde , Marija Vucelja , Tiberiu Tesileanu , Vijay Balasubramanian

Some bacteria and archaea possess an immune system, based on the CRISPR-Cas mechanism, that confers adaptive immunity against phage. In such species, individual bacteria maintain a "cassette" of viral DNA elements called spacers as a memory…

种群与进化 · 定量生物学 2022-06-08 Serena Bradde , Armita Nourmohammad , Sidhartha Goyal , Vijay Balasubramanian

Clustered regularly interspaced short palindromic repeats (CRISPR) in bacterial and archaeal DNA have recently been shown to be a new type of anti-viral immune system in these organisms. We here study the diversity of spacers in CRISPR…

种群与进化 · 定量生物学 2010-08-17 Jiankui He , Michael W. Deem

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), linked with CRISPR associated (CAS) genes, play a profound role in the interactions between phage and their bacterial hosts. It is now well understood that CRISPR-CAS…

种群与进化 · 定量生物学 2016-03-18 Qasim Ali , Lindi Wahl

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells. A heritable, molecular memory is…

种群与进化 · 定量生物学 2018-03-28 Melia E. Bonomo , Michael W. Deem

We estimate the number of spacers in a CRISPR array of a bacterium which maximizes its protection against a viral attack. The optimality follows from a competition between two trends: too few distinct spacers make the bacteria vulnerable to…

种群与进化 · 定量生物学 2017-12-22 Alexander Martynov , Konstantin Severinov , Yaroslav Ispolatov

CRISPR is a newly discovered prokaryotic immune system. Bacteria and archaea with this system incorporate genetic material from invading viruses into their genomes, providing protection against future infection by similar viruses. The…

种群与进化 · 定量生物学 2017-08-18 Pu Han , Michael W. Deem

Biological organisms have evolved a wide range of immune mechanisms to defend themselves against pathogens. Beyond molecular details, these mechanisms differ in how protection is acquired, processed and passed on to subsequent generations…

种群与进化 · 定量生物学 2016-12-26 Andreas Mayer , Thierry Mora , Olivier Rivoire , Aleksandra M. Walczak

The immune system normally protects the human host against death by infection. However, when an immune response is mistakenly directed at self antigens, autoimmune disease can occur. We describe a model of protein evolution to simulate the…

种群与进化 · 定量生物学 2009-11-11 Jun Sun , David J. Earl , Michael W. Deem

To ensure faithful information transmission, cells utilize nonequilibrium drives to reduce errors. Kinetic proofreading is a classic mechanism known to sharpen ligand discrimination by T lymphocytes. It remains an open question whether the…

生物物理 · 物理学 2025-09-23 Qing Xu , Shenshen Wang

CRISPR-Cas is an adaptive immune mechanism that has been harnessed for a variety of genetic engineering applications: the Cas9 protein recognises a 2-5nt DNA motif, known as the PAM, and a programmable crRNA binds a target DNA sequence that…

生物物理 · 物理学 2021-01-06 Melia E. Bonomo

Background: The development of classification methods for personalized medicine is highly dependent on the identification of predictive genetic markers. In survival analysis it is often necessary to discriminate between influential and…

统计方法学 · 统计学 2018-02-27 Thomas Welchowski , Verena Zuber , Matthias Schmid

Deep learning models for Electrocardiogram (ECG) diagnosis have achieved remarkable accuracy but exhibit fragility against adversarial perturbations, particularly Smooth Adversarial Perturbations (SAP) that mimic biological morphology.…

机器学习 · 计算机科学 2026-01-01 Shunbo Jia , Caizhi Liao

Clonal structure of the human peripheral T-cell repertoire is shaped by a number of homeostatic mechanisms, including antigen presentation, cytokine and cell regulation. Its accurate tuning leads to a remarkable ability to combat pathogens…

定量方法 · 定量生物学 2014-10-07 O. V. Bolkhovskaya , D. Yu. Zorin , M. V. Ivanchenko
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