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Deep learning models that are trained on histopathological images obtained from a single lab and/or scanner give poor inference performance on images obtained from another scanner/lab with a different staining protocol. In recent years,…

图像与视频处理 · 电气工程与系统科学 2020-10-07 Harshal Nishar , Nikhil Chavanke , Nitin Singhal

Histological staining is the gold standard for tissue examination in clinical pathology and life-science research, which visualizes the tissue and cellular structures using chromatic dyes or fluorescence labels to aid the microscopic…

医学物理 · 物理学 2023-03-07 Bijie Bai , Xilin Yang , Yuzhu Li , Yijie Zhang , Nir Pillar , Aydogan Ozcan

Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation…

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

图像与视频处理 · 电气工程与系统科学 2020-02-06 Pegah Salehi , Abdolah Chalechale

Histopathological images (HIs) are the gold standard for evaluating some types of tumors for cancer diagnosis. The analysis of such images is not only time and resource consuming, but also very challenging even for experienced pathologists,…

Accurate histopathological diagnosis often requires multiple differently stained tissue sections, a process that is time-consuming, labor-intensive, and environmentally taxing due to the use of multiple chemical stains. Recently, virtual…

计算机视觉与模式识别 · 计算机科学 2025-09-18 Jiabo MA , Wenqiang Li , Jinbang Li , Ziyi Liu , Linshan Wu , Fengtao Zhou , Li Liang , Ronald Cheong Kin Chan , Terence T. W. Wong , Hao Chen

Stains are essential in histopathology to visualize specific tissue characteristics, with Haematoxylin and Eosin (H&E) serving as the clinical standard. However, pathologists frequently utilize a variety of special stains for the diagnosis…

计算机视觉与模式识别 · 计算机科学 2026-02-11 Oskar Thaeter , Christian Grashei , Anette Haas , Elisa Schmoeckel , Han Li , Peter J. Schüffler

In addition to evaluating tumor morphology using H&E staining, immunohistochemistry is used to assess the presence of specific proteins within the tissue. However, this is a costly and labor-intensive technique, for which virtual staining,…

计算机视觉与模式识别 · 计算机科学 2025-11-25 José Teixeira , Pascal Klöckner , Diana Montezuma , Melis Erdal Cesur , João Fraga , Hugo M. Horlings , Jaime S. Cardoso , Sara P. Oliveira

There is a strong need for automated systems to improve diagnostic quality and reduce the analysis time in histopathology image processing. Automated detection and classification of pathological tissue characteristics with computer-aided…

计算机视觉与模式识别 · 计算机科学 2019-11-22 Muhammed Talo

It is commonly recognized that color variations caused by differences in stains is a critical issue for histopathology image analysis. Existing methods adopt color matching, stain separation, stain transfer or the combination of them to…

图像与视频处理 · 电气工程与系统科学 2022-08-09 Hai-Li Ye , Da-Han Wang

Virtual staining offers a promising method for converting Hematoxylin and Eosin (H&E) images into Immunohistochemical (IHC) images, eliminating the need for costly chemical processes. However, existing methods often struggle to utilize…

计算机视觉与模式识别 · 计算机科学 2025-11-11 Sicheng Yang , Zhaohu Xing , Haipeng Zhou , Lei Zhu

The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

We propose a virtual staining methodology based on Generative Adversarial Networks to map histopathology images of breast cancer tissue from H&E stain to PHH3 and vice versa. We use the resulting synthetic images to build Convolutional…

图像与视频处理 · 电气工程与系统科学 2020-03-18 Caner Mercan , Germonda Reijnen-Mooij , David Tellez Martin , Johannes Lotz , Nick Weiss , Marcel van Gerven , Francesco Ciompi

Stain color variation in histological images, caused by a variety of factors, is a challenge not only for the visual diagnosis of pathologists but also for cell segmentation algorithms. To eliminate the color variation, many stain…

图像与视频处理 · 电气工程与系统科学 2022-10-27 Huaqian Wu , Nicolas Souedet , Camille Mabillon , Caroline Jan , Cédric Clouchoux , Thierry Delzescaux

Color inconsistency is an inevitable challenge in computational pathology, which generally happens because of stain intensity variations or sections scanned by different scanners. It harms the pathological image analysis methods, especially…

图像与视频处理 · 电气工程与系统科学 2022-03-01 Bingchao Zhao , Jiatai Lin , Changhong Liang , Zongjian Yi , Xin Chen , Bingbing Li , Weihao Qiu , Danyi Li , Li Liang , Chu Han , Zaiyi Liu

The color appearance of a pathological image is highly related to the imaging protocols, the proportion of different dyes, and the scanning devices. Computer-aided diagnostic systems may deteriorate when facing these color-variant…

图像与视频处理 · 电气工程与系统科学 2025-06-25 Zheng Chen

Medical image analysis has become a topic under the spotlight in recent years. There is a significant progress in medical image research concerning the usage of machine learning. However, there are still numerous questions and problems…

图像与视频处理 · 电气工程与系统科学 2018-05-16 Taha J. Alhindi , Shivam Kalra , Ka Hin Ng , Anika Afrin , Hamid R. Tizhoosh

In the present study, we propose a novel case-based similar image retrieval (SIR) method for hematoxylin and eosin (H&E)-stained histopathological images of malignant lymphoma. When a whole slide image (WSI) is used as an input query, it is…

In this paper, we develop a complete pipeline for stain normalization, segmentation, and classification of nuclei in hematoxylin and eosin (H&E) stained breast cancer histopathology images. In the first step, we use a CNN-based stain…

计算机视觉与模式识别 · 计算机科学 2018-11-12 Edwin Yuan , Junkyo Suh

Segmentation of nuclei regions from histological images is an important task for automated computer-aided analysis of histological images, particularly in the presence of impermissible color variation in the color appearance of stained…

图像与视频处理 · 电气工程与系统科学 2025-06-10 Suman Mahapatra , Pradipta Maji