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Networks of filamentous proteins play a crucial role in cell mechanics. These cytoskeletal networks, together with various crosslinking and other associated proteins largely determine the (visco)elastic response of cells. In this letter we…

无序系统与神经网络 · 物理学 2009-11-10 D. A. Head , A. J. Levine , F. C. MacKintosh

The proper functions of tissues depend on the ability of cells to withstand stress and maintain shape. Central to this process is the cytoskeleton, comprised of three polymeric networks: F-actin, microtubules, and intermediate filaments.…

生物物理 · 物理学 2020-08-25 Alison E. Patteson , Robert J. Carroll , Daniel V. Iwamoto , Paul A. Janmey

The mechanical properties of cells are dominated by the cytoskeleton, an interconnected network of long elastic filaments. The connections between the filaments are provided by crosslinking proteins, which constitute, next to the filaments,…

软凝聚态物质 · 物理学 2012-10-09 Claus Heussinger

Recent experiments have demonstrated that the nonlinear elasticity of in vitro networks of the biopolymer actin is dramatically altered in the presence of a flexible cross-linker such as the abundant cytoskeletal protein filamin. The basic…

软凝聚态物质 · 物理学 2015-05-13 C. P. Broedersz , C. Storm , F. C. MacKintosh

Intermediate filaments form an essential structural network, spread throughout the cytoplasm and play a key role in cell mechanics, intracellular organization and molecular signaling. The maintenance of the network and its adaptation to the…

亚细胞过程 · 定量生物学 2023-06-14 Youngmin Park , Cécile Leduc , Sandrine Etienne-Manneville , Stéphanie Portet

We use optical tweezers microrheology and fluorescence microscopy to apply nonlinear microscale strains to entangled and crosslinked actin networks, and measure the resulting stress and actin filament deformations. We couple nonlinear…

软凝聚态物质 · 物理学 2016-10-24 Bekele Gurmessa , Shea Ricketts , Rae M. Robertson-Anderson

Disordered filamentous networks with compliant crosslinks exhibit a low linear elastic shear modulus at small strains, but stiffen dramatically at high strains. Experiments have shown that the elastic modulus can increase by up to three…

The migration of cells through tight constricting spaces or along fibrous tracks in tissues is important for biological processes, such as embryogenesis, wound healing, and cancer metastasis, and depends on the mechanical properties of the…

Strain stiffening of protein networks is explored by means of a finite strain analysis of a two-dimensional network model of cross-linked semiflexible filaments. The results show that stiffening is caused by non-affine network…

软凝聚态物质 · 物理学 2009-11-11 P. R. Onck , T. Koeman , T. Van Dillen , E. Van der Giessen

Motivated by recent experiments showing nonlinear elasticity of in vitro networks of the biopolymer actin cross-linked with filamin, we present an effective medium theory of flexibly cross-linked stiff polymer networks. We model such…

软凝聚态物质 · 物理学 2008-10-19 C. P. Broedersz , C. Storm , F. C. MacKintosh

We have studied the elastic response of actin networks with both compliant and rigid crosslinks by modeling molecular motors as force dipoles. Our finite element simulations show that for compliant crosslinkers such as filamin A, the…

软凝聚态物质 · 物理学 2010-09-06 Peng Chen , Vivek B. Shenoy

In cells, cytoskeletal filament networks are responsible for cell movement, growth, and division. Filaments in the cytoskeleton are driven and organized by crosslinking molecular motors. In reconstituted cytoskeletal systems, motor activity…

We introduce a mathematical model for the mechanical behaviour of the eukaryotic cell cytoskeleton. This discrete model involves a regular array of pre-stressed protein filaments that exhibit resistance to enthalpic stretching, joined at…

定量方法 · 定量生物学 2023-09-06 J. Köry , N. A. Hill , X. Y. Luo , P. S. Stewart

Disordered networks of semiflexible filaments are common support structures in biology. Familiar examples include fibrous matrices in blood clots, bacterial biofilms, and essential components of cells and tissues of plants, animals, and…

软凝聚态物质 · 物理学 2023-09-28 Saamiya Syed , Fred C. MacKintosh , Jordan L. Shivers

The synthetic biopolymeric gels demand a great interest as bio-materials to mimic many biological scaffolding structures, which can contribute to a better understanding of the cytoskeleton-like structural building blocks and soft…

软凝聚态物质 · 物理学 2019-05-01 Horacio Lopez-Menendez , Libardo Gonzalez-Torres

The semiflexible F-actin network of the cytoskeleton is cross-linked by a variety of proteins including filamin, which contain Ig-domains that unfold under applied tension. We examine a simple semiflexible network model cross-linked by such…

软凝聚态物质 · 物理学 2009-11-11 B. A. DiDonna , Alex J. Levine

Bundles of polymer filaments are responsible for the rich and unique mechanical behaviors of many biomaterials, including cells and extracellular matrices. In fibrin biopolymers, whose nonlinear elastic properties are crucial for normal…

We present a theoretical framework for the linear and nonlinear visco-elastic properties of reversibly crosslinked networks of semiflexible polymers. In contrast to affine models where network strain couples to the polymer end-to-end…

软凝聚态物质 · 物理学 2016-07-06 Jan Plagge , Andreas Fischer , Claus Heussinger

We study the mechanical stiffening behavior in two-dimensional (2D) cross-linked networks of semiflexible biopolymer filaments under simple shear. Filamental constituents immersed in a fluid undergo thermally excited bending motions.…

生物物理 · 物理学 2007-05-23 T. Van Dillen , P. R. Onck , E. Van der Giessen

Experiments have shown that elasticity of disordered filamentous networks with compliant crosslinks is very different from networks with rigid crosslinks. Here, we model and analyze filamentous networks as a collection of randomly oriented…

生物物理 · 物理学 2015-06-17 A. Sharma , M. Sheinman , K. M. Heidemann , F. C. MacKintosh
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