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Confined hydration and conformational flexibility are some of the challenges encountered for the rational design of selective antagonists of G-protein coupled receptors. We present a set of C3-substituted (-)-stepholidine derivatives as…

Computing equilibrium concentrations of molecular complexes is generally analytically intractable and requires numerical approaches. In this work we focus on the polymer-monomer level, where indivisible molecules (monomers) combine to form…

数据结构与算法 · 计算机科学 2025-08-25 Hamidreza Akef , Minki Hhan , David Soloveichik

Understanding how different classes of molecules move across biological membranes is a prerequisite to predicting a solute's permeation rate, which is a critical factor in the fields of drug design and pharmacology. We use biased Molecular…

生物物理 · 物理学 2018-05-16 Nihit Pokhrel , Lutz Maibaum

The adhesion of cells is mediated by receptors and ligands anchored in apposing membranes. A central question is how to characterize the binding affinity of these membrane-anchored molecules. For soluble molecules, the binding affinity is…

软凝聚态物质 · 物理学 2009-06-03 Heinrich Krobath , Bartosz Rozycki , Reinhard Lipowsky , Thomas R. Weikl

We introduce a derangement model of ligand-receptor binding that allows us to quantitatively frame the question "How can ligands seek out and bind to their optimal receptor sites in a sea of other competing ligands and suboptimal receptor…

生物大分子 · 定量生物学 2022-08-24 Mobolaji Williams

Understanding design principles of biomolecular recognition is a key question of molecular biology. Yet the enormous complexity and diversity of biological molecules hamper the efforts to gain a predictive ability for the free energy of…

生物大分子 · 定量生物学 2012-04-19 Michael Elkin , Ingemar Andre , David B. Lukatsky

In multi-resolution simulations, different system components are simultaneously modelled at different levels of resolution, these being smoothly coupled together. In the case of enzyme systems, computationally expensive atomistic detail is…

生物物理 · 物理学 2016-11-03 Aoife C. Fogarty , Raffaello Potestio , Kurt Kremer

The phase separation in lipid bilayers that include negatively charged lipids is examined experimentally. We observed phase-separated structures and determined the membrane miscibility temperatures in several binary and ternary lipid…

软凝聚态物质 · 物理学 2014-10-16 Hiroki Himeno , Naofumi Shimokawa , Shigeyuki Komura , David Andelman , Tsutomu Hamada , Masahiro Takagi

Free energy calculations are rapidly becoming indispensable in structure-enabled drug discovery programs. As new methods, force fields, and implementations are developed, assessing their expected accuracy on real-world systems…

The concept of a protein diffusing in its free energy folding landscape has been fruitful for both theory and experiment. Yet the choice of the reaction coordinate (RC) introduces an undesirable degree of arbitrariness into the problem. We…

生物物理 · 物理学 2015-05-19 Michael Hinczewski , Yann von Hansen , Joachim Dzubiella , Roland R. Netz

Although the importance of protein dynamics in protein function is generally recognized, the role of protein fluctuations in allosteric effects scarcely has been considered. To address this gap, the Kullback-Leibler divergence (Dx) between…

生物大分子 · 定量生物学 2009-11-13 Michael E. Wall

We present the multicomponent functionalized free energies that characterize the low-energy packings of amphiphilic molecules within a membrane through a correspondence to connecting orbits within a reduced dynamical system. To each…

偏微分方程分析 · 数学 2020-10-28 Yuan Chen , Arjen Doelman , Keith Promislow , Frits Veerman

Alchemical Grid Dock (AlGDock) is open-source software designed to compute the binding potential of mean force (BPMF) - the binding free energy between a flexible ligand and a rigid receptor - for a small organic ligand and a biological…

化学物理 · 物理学 2020-06-30 David D. L Minh

Absolute Binding Free Energy (ABFE) methods are among the most accurate computational techniques for predicting protein-ligand binding affinities, but their utility is limited by the need for many simulations of alchemically modified…

定量方法 · 定量生物学 2026-03-18 Michael Brocidiacono , Brandon Novy , Rishabh Dey , Konstantin I. Popov , Alexander Tropsha

The massively parallel computation of absolute binding free energy with a well-equilibrated system (MP-CAFEE) has been developed [H. Fujitani, Y. Tanida, M. Ito, G. Jayachandran, C. D. Snow, M. R. Shirts, E. J. Sorin, and V. S. Pande, J.…

软凝聚态物质 · 物理学 2009-11-13 Yoshiaki Tanida , Masakatsu Ito , Hideaki Fujitani

The molecular distributions obtained from canonical Monte Carlo simulations can be used to find an approximate interaction energy. This serves as the basis of a method for estimating the binding free energy for a ligand to a protein which…

化学物理 · 物理学 2007-05-23 Charles F. F. Karney , Jason E. Ferrara , Clay D. Spence

We present a dynamical model of lipoprotein metabolism derived by combining a cascading process in the blood stream and cellular level regulatory dynamics. We analyse the existence and stability of equilibria and show that this…

定量方法 · 定量生物学 2007-05-23 Elias August , Kim H. Parker , Mauricio Barahona

The efficacy of drug treatments depends on how tightly small molecules bind to their target proteins. The rapid and accurate quantification of the strength of these interactions (as measured by binding affinity) is a grand challenge of…

分布式、并行与集群计算 · 计算机科学 2018-10-30 Jumana Dakka , Kristof Farkas-Pall , Matteo Turilli , David W Wright , Peter V Coveney , Shantenu Jha

Field-theoretical method is proposed, that yields analytical expression for lateral pressure distribution across hydrophobic core of a bilayer lipid membrane. Lipid molecule, repelling entropicaly surrounding neighbors, is modeled as a…

软凝聚态物质 · 物理学 2007-05-23 Sergei I. Mukhin , Svetlana V. Baoukina

The prediction of protein-ligand binding affinity is of great significance for discovering lead compounds in drug research. Facing this challenging task, most existing prediction methods rely on the topological and/or spatial structure of…

生物大分子 · 定量生物学 2022-09-28 Yang Zhang , Gengmo Zhou , Zhewei Wei , Hongteng Xu