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Fluorescence-detected Fourier transform (FT) spectroscopy is a technique in which the relative paths of an optical interferometer are controlled to excite a material sample, and the ensuing fluorescence is detected as a function of the…

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Detector characterization and instrumentation testing are often performed at cyclotron and synchrotron facilities, many of which were originally developed for medical applications in cancer therapy. For particle physics experiments…

Single-photon detection and photon counting play a central role in a large number of quantum communication and computation protocols. While the efficiency of state-of-the-art photo-detectors is well below the desired limits, quantum state…

量子物理 · 物理学 2009-11-07 A. Imamoglu

The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion of membrane receptors and the kinetics of transcription factors governing the rates of gene expression. Different fluorescence…

定量方法 · 定量生物学 2020-04-03 Maxime Woringer , Ignacio Izeddin , Cyril Favard , Hugues Berry

After receiving an X-ray photon, an X-ray detector is not operational for a duration known as deadtime. It is detector specific and its effect on the data depends upon the luminosity of the source. It reduces the observed photon count rate…

高能天体物理现象 · 物理学 2024-08-27 M. Vivekanand

Optical observations with high time resolution are essential for understanding the origin of sub-millisecond timescale astronomical phenomena, including giant radio pulses from the Crab Pulsar. We have developed a high-speed imaging system…

This paper reported a bespoke adder-based deep learning network for time-domain fluorescence lifetime imaging (FLIM). By leveraging the l1-norm extraction method, we propose a 1-D Fluorescence Lifetime AdderNet (FLAN) without…

信号处理 · 电气工程与系统科学 2022-09-13 Zhenya Zang , Dong Xiao , Quan Wang , Ziao Jiao , Chen Yu , David Day-Uei Li

The increasing complexity of the recent photonic experiments challenges developing efficient multi-channel coincidence counting systems with high-level functionality. Here, we report a coincidence unit able to count detection events ranging…

光学 · 物理学 2024-02-29 Josef Hloušek , Jan Grygar , Michal Dudka , Miroslav Ježek

Aberrations and multiple scattering in biological tissues critically distort light beams into highly complex speckle patterns. In this regard, digital optical phase conjugation (DOPC) is a promising technique enabling in-depth focusing.…

Photon correlation spectroscopy (PCS) is based on measuring the temporal correlation of the light intensity scattered by the investigated sample. A typical setup requires a temporally coherent light source. Here, we show that a…

光学 · 物理学 2015-05-30 D. Salerno , D. Brogioli , F. Croccolo , R. Ziano , F. Mantegazza

This paper presents a new Bayesian model and algorithm used for depth and intensity profiling using full waveforms from the time-correlated single photon counting (TCSPC) measurement in the limit of very low photon counts. The model…

应用统计 · 统计学 2016-04-20 Yoann Altmann , Ximing Ren , Aongus McCarthy , Gerald S. Buller , Steve McLaughlin

We study a simple modification to the conventional time of flight mass spectrometry (TOFMS) where a \emph{variable} and (pseudo)-\emph{random} pulsing rate is used which allows for traces from different pulses to overlap. This modification…

最优化与控制 · 数学 2013-07-30 Morteza Ibrahimi , Andrea Montanari , George S Moore

Detecting brief changes in time-series data remains a major challenge in fields where short-lived states carry meaning. In single-molecule localisation microscopy, this problem is particularly acute as fluorescent molecules used to tag…

统计方法学 · 统计学 2026-03-25 Emily Gribbin , Benjamin Davis , Daniel Rolfe , Hannah Mitchell

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

计算机视觉与模式识别 · 计算机科学 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

Photonic lattices - arrays of optical waveguides - are powerful platforms for simulating a range of phenomena, including topological phases. While probing dynamics is possible in these systems, by reinterpreting the propagation direction as…

-In the Alvarez-Macovski method [R.E. Alvarez and A. Macovski, Phys. Med. Biol., 1976, 733-744], the attenuation coefficient is approximated as a linear combination of functions of energy multiplied by coefficients that depend on the…

仪器与探测器 · 物理学 2017-02-17 Robert Alvarez

Photon number resolving detectors are needed for a variety of applications including linear-optics quantum computing. Here we describe the use of time-multiplexing techniques that allows ordinary single photon detectors, such as silicon…

量子物理 · 物理学 2007-05-23 M. J. Fitch , B. C. Jacobs , T. B. Pittman , J. D. Franson

Lidar with direct time-of-flight (dToF) technology based on single-photon avalanche diode detectors (SPAD) has been widely adopted in various applications. However, a comprehensive theoretical understanding of its fundamental ranging…

光学 · 物理学 2025-10-09 Hao Wu , Shiyi Sun , Lijie Zhao , Yingyu Wang , Limin Tong , Linjie Shen

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…