相关论文: Standing-wave-excited multiplanar fluorescence in …
Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…
3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…
Photobleaching is a major limitation of superresolution Stimulated Depletion Emission (STED) microscopy. Fast scanning has long been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful…
With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the…
In this work we present a modification to conventional X-rays fluorescence using electrons as excitation source, and compare it with the traditional X-ray excitation for the study of pigments. For this purpose we have constructed a…
Evanescent-wave microscopy achieves sub-diffraction axial sectioning by confining fluorescence excitation to a thin layer close to the cell/substrate interface. How thin this light sheet exactly is, however, is often unknown. Particularly…
Recent observations of reflected propagating and standing slow-mode waves in hot flaring coronal loops have spurred our investigation into their underlying excitation and damping mechanisms. To understand these processes, we conduct 2.5D…
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…
Infrared optical measurement has a wide range of applications in industry and science, but infrared light detectors suffer from high costs and inferior performance than visible light detectors. Four-wave mixing (FWM) process allows…
Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample excitation.…
The OpenFlexure Microscope is a 3D printed, low-cost microscope capable of automated image acquisition through the use of a motorised translation stage and a Raspberry Pi imaging system. This automation has applications in research and…
We propose a new design for a multi-color, parallelized STED microscope, capable of multiple beam scanning. Our design is based on a common programmable diffracting optical element used to split, shape, and align both the excitation beams…
Several matrix approaches were developed to control light propagation through multiple scattering media under illumination of ultrashort pulses of light. These matrices can be recorded either with spectral or temporal resolution. Thanks to…
Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…
Single dye molecules at cryogenic temperatures display many spectroscopic phenomena known from free atoms and are thus promising candidates for fundamental quantum optical studies. However, the existing techniques for the detection of…
Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…
Using optical speckle scanning microscopy [1], we demonstrate that clear images of multiple cells can be obtained through biological scattering tissue, with subcellular resolution and good image quality, as long as the size of the imaging…
Stimulated amplification of fluorescence signals is obtained under conditions which approach biocompatibility by adding NanoParticles (NPs) to an aqueous solution of fluorescein. The conditions for the stability of the suspension and…
We propose an experimental arrangement to image, with attosecond resolution, transient surface plasmonic excitations. The required modifications to state-of-the-art setups used for attosecond streaking experiments from solid surfaces only…
The problem of standing wave formation by superposing two counter-propagating whistler waves in an overdense plasma, studied recently by Sano et al. (Phys. Rev. E 100, 053205 (2019) and Phys. Rev. E 101, 013206 (2020)), has been revisited…