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We review a series of experimental studies of the thermodynamics of nonequilibrium processes at the microscale. In particular, in these experiments we studied the fluctuations of the thermodynamic properties of a single optically-trapped…

统计力学 · 物理学 2016-06-22 L. Dinis , I. A. Martínez , É. Roldán , J. M. R. Parrondo , R. A. Rica

The objective of this project is to develop an automated device used to spot protein samples on a hydrophobic membrane to be used for the patented electrophoresis method developed by Chang and Yonan in 2008 [1]. This novel method performs…

其他定量生物学 · 定量生物学 2023-03-13 Christopher M. Altenderfer , Frank N. Chang , Parsaoran Hutapea

Non-invasive optical manipulation of particles has emerged as a powerful and versatile tool for biological study and nanotechnology. In particular, trapping and rotation of cells, cell nuclei and sub-micron particles enables unique…

Active and diffusive motion in Brownian particles are regularly observed in fluidic environments, albeit at different time scales. Here, we experimentally study the dynamics of highly asymmetric microclusters trapped in air employing…

光学 · 物理学 2025-04-01 Anita Pahi , Kirty Ranjan Sahoo , Biswajit Das , Shuvojit Paul , Ayan Banerjee

We report initial observations of coherent optical transition radiation interferometry (COTRI) patterns generated by microbunched electrons from laser-driven plasma accelerators (LPAs). These are revealed in the angular distribution…

The determination of the mean first passage time (MFPT) for a Brownian particle in a bounded 2-D domain containing small absorbing traps is a fundamental problem with biophysical applications. The average MFPT is the expected capture time…

统计力学 · 物理学 2020-06-24 Sarafa A. Iyaniwura , Tony Wong , Colin B. Macdonald , Micheal J. Ward

The operation of a BEC based atom interferometer, where the atoms are held in a weakly-confining magnetic trap and manipulated with counter-propagating laser beams, is analyzed. A simple analytic model is developed to describe the dynamics…

原子物理 · 物理学 2009-11-13 James A. Stickney , Rudra P. Kafle , Dana Z. Anderson , Alex A. Zozulya

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this review, we examine different techniques of…

生物大分子 · 定量生物学 2007-05-23 Ivan Rasnik , Sean A. McKinney , Taekjip Ha

Continuous tuning of the frequency of laser light is the cornerstone for a plethora of applications in basic science as well as in the industrial environment. They range from the hunt for gravitational waves, the realization of optical…

光学 · 物理学 2023-11-22 Alexander Mrokon , Johanna Oehler , Ingo Breunig

Adenosine triphosphate (ATP) is a highly energetic molecule used as a coenzyme in the transfer of chemical energy required for the continuation of cellular metabolism. In addition, extracellular ATP has an important role in cell-to-cell…

医学物理 · 物理学 2018-10-17 Mustafa Sen

Absorbing aerosols, such as brown carbon (BrC) and absorbing secondary organic aerosols (SOA), has attracted broad interest due to their importance for climate and human health. The pronounced time-dependence of light absorption during…

光学 · 物理学 2026-05-18 Krispin M. Dettlaff , James Wenger , Grégory David , Ruth Signorell

The paper presents and experimentally investigates a concept of an efficient radiation trap based on adiabatic photon confinement between curved mirrors. Such a trap is free of restrictions typical to the Fabry-Perot cells, relating to the…

仪器与探测器 · 物理学 2017-08-28 S. S. Popov , M. G. Atluhanov , A. V. Burdakov , M. Yu. Ushkova

We experimentally realize quasistatic adiabatic processes using a single optically-trapped micro- sphere immersed in water whose effective temperature is controlled by an external random electric field. A full energetic characterization of…

统计力学 · 物理学 2015-06-03 Ignacio A. Martínez , Édgar Roldán , Luis Dinis , Dmitri Petrov , Raúl A. Rica

The dielectric breakdown approach for forming nanopores has greatly accelerated the pace of research in solid-state nanopore sensing, enabling inexpensive formation of nanopores via a bench top setup. Here we demonstrate the potential of…

Light-phase-sensitive techniques, such as coherent multidimensional spectroscopy, are well-established in a broad spectral range, already spanning from radio-frequencies in nuclear magnetic resonance spectroscopy to visible and ultraviolet…

The active Brownian particle (ABP) model describes a swimmer, synthetic or living, whose direction of swimming is a Brownian motion. The swimming is due to a propulsion force, and the fluctuations are typically thermal in origin. We present…

软凝聚态物质 · 物理学 2022-07-18 Jean-Luc Thiffeault , Jiajia Guo

A single-biomolecule optical toggle-switch is demonstrated based on a mutated green fluorescent protein (GFP). We have exploited molecular biology techniques to tailor GFP molecular structure and photophysical properties and give it…

Confocal time resolved single-molecule spectroscopy using pulsed laser excitation and synchronized multi channel time correlated single photon counting (TCSPC) provides detailed information about the conformational changes of a biological…

生物物理 · 物理学 2009-11-13 N. Zarrabi , M. G. Dueser , S. Ernst , R. Reuter , G. D. Glick , S. D. Dunn , J. Wrachtrup , M. Boersch

In this paper, we present a technique for magneto-optical cooling and trapping of neutral atoms using a single laser. The alternating-frequency magneto-optical trap (AF-MOT) uses an agile light source that sequentially switches between…

原子物理 · 物理学 2019-10-15 B. Wiegand , B. Leykauf , K. Döringshoff , Y. D. Gupta , A. Peters , M. Krutzik

FoF1-ATP synthase is the enzyme that provides the 'chemical energy currency' adenosine triphosphate, ATP, for living cells. The formation of ATP is accomplished by a stepwise internal rotation of subunits within the enzyme. Briefly, proton…

生物大分子 · 定量生物学 2009-11-13 N. Zarrabi , S. Ernst , M. G. Dueser , A. Golovina-Leiker , W. Becker , R. Erdmann , S. D. Dunn , M. Borsch