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The Penning-trap electronic-detection technique that offers the precision and sensitivity requested in mass spectrometry for fundamental studies in nuclear and particle physics has not been proven yet to be universal. This has motivated the…

There is a trend in research towards more automation using smart systems powered by artificial intelligence. While experiments are often challenging to automate, they can greatly benefit from automation by reducing labor and increasing…

Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool for analyzing diffusion and binding processes. Confocal scanning laser microscopes that are used in FRAP experiments bleach regions with a radially…

亚细胞过程 · 定量生物学 2009-03-09 George D Tsibidis

The enzyme FoF1-ATP synthase provides the 'chemical energy currency' adenosine triphosphate (ATP) for living cells. Catalysis is driven by mechanochemical coupling of subunit rotation within the enzyme with conformational changes in the…

生物大分子 · 定量生物学 2015-06-04 Stefan Ernst , Monika G. Dueser , Nawid Zarrabi , Michael Boersch

Brownian ratchet has emerged as a promising tool for understanding motion mechanism of molecules and proteins, and dynamically manipulating particles in non-equilibrium thermodynamics state. Here, we propose and experimentally demonstrate a…

光学 · 物理学 2021-06-02 Xionggui Tang , Yanhua Xu

Accurate detection and characterization of nanoparticles within confined spaces is crucial for applications ranging from nanofluidics to biotechnology. We present a novel approach that combines interferometric scattering (iSCAT) detection…

Single particle diffraction imaging experiments at free-electron lasers (FEL) have a great potential for structure determination of reproducible biological specimens that can not be crystallized. One of the challenges in processing the data…

生物物理 · 物理学 2013-10-16 O. M. Yefanov , I. A. Vartanyants

Optical metasurfaces have been extensively investigated, demonstrating diverse and multiple functionalities with complete control over the transmitted and reflected fields. Most optical metasurfaces are however static, with only a few…

We investigate the local fluctuations of filamentous actin (F-actin), with focus on the skeletal thin filament, using single-particle optical trapping interferometry. This experimental technique allows us to detect the Brownian motion of a…

软凝聚态物质 · 物理学 2024-05-27 Pablo Domínguez-García , Jose R. Pinto , Ana Akrap , Sylvia Jeney

We present the design and fabrication of an endcap-type Paul trap. The trap is designed for studies with Ca$^{+}$ and Yb$^{+}$. The design, fabrication process, and characterization are presented in detail with a focus on trapping a single…

A colloidal suspension of active Brownian particles (ABPs) driven by controllable forces into directed or persistent motions can serve as a model for understanding the biological systems. Experiments and numerical simulations are…

软凝聚态物质 · 物理学 2019-08-27 Chong Shen , Lanfang Li , Zhiyu Jiang , H. Daneil Ou-Yang

Protein function and activity are a consequence of its three-dimensional structure. Single particle analysis of cryogenic electron micrographs has radically changed structural biology allowing atomic reconstruction of almost any type of…

生物大分子 · 定量生物学 2019-07-09 Gabriele Marchello , Cesare De Pace , Neil Wilkinson , Lorena Ruiz-Perez , Giuseppe Battaglia

Photosensitive proteins embedded in the cell membrane (about 5 nm thickness) act as photoactivated proton pumps, ion gates, enzymes, or more generally, as initiators of stimuli for the cell activity. They are composed of a protein backbone…

With the aim to parallelize and monitor biological or biochemical phenomena, trapping and immobilization of objects such as particles, droplets or cells in microfluidic devices has been an intense area of research and engineering so far.…

Advances in cryogenic sciences have enabled several observations of new low-temperature physical phenomena including superconductivity, superfluidity, and Bose-Einstein condensates. Heat transfer is also critical in numerous applications…

介观与纳米尺度物理 · 物理学 2015-03-26 Paden B. Roder , Bennett E. Smith , Xuezhe Zhou , Matthew J. Crane , Peter J. Pauzauskie

The local viscoelastic (VE) environment governs the motion of an embedded microsphere and consequently, pertinent dynamical phenomena. However, studying such phenomena with varying VE properties remains challenging for various reasons,…

软凝聚态物质 · 物理学 2026-03-10 Sanatan Halder , Manas Khan

In this article we explore the dynamics of a Brownian particle in a feedback-free dynamic thermophoretic trap. The trap contains a focused laser beam heating a circular gold structure locally and creating a repulsive thermal potential for a…

软凝聚态物质 · 物理学 2017-09-13 Marco Braun , Alois Würger , Frank Cichos

Temporally and spatially resolved measurements of protein transport inside cells provide important clues to the functional architecture and dynamics of biological systems. Fluorescence Recovery After Photobleaching (FRAP) technique has been…

亚细胞过程 · 定量生物学 2009-03-04 George D. Tsibidis

Synchrotron x-ray scattering studies of anti-ferroelectric nanoregions (AFR) as fluctuations, which give rise to 1/2(hk0) superlattice reflections (alpha spots), were systematically performed in Pb(Mg1/3Nb2/3)O3 (PMN) ferroelectric relaxor.…

材料科学 · 物理学 2007-05-23 A. Tkachuk , Haydn Chen

Atomic Beam Probe (ABP) is an extension of the routinely used Beam Emission Spectroscopy (BES) diagnostic for plasma edge current fluctuation measurement at magnetically confined plasmas. Beam atoms ionized by the plasma are directed to a…