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Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

计算机视觉与模式识别 · 计算机科学 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Rapid advance of experimental techniques provides an unprecedented in-depth view into complex developmental processes. Still, little is known on how the complexity of multicellular organisms evolved by elaborating developmental programs and…

分子网络 · 定量生物学 2021-07-16 Somya Mani , Tsvi Tlusty

Modeling the 3D structures of cells and tissues is crucial in biology. Sequential cross-sectional images from electron microscopy provide high-resolution intracellular structure information. The segmentation of complex cell structures…

定量方法 · 定量生物学 2025-02-14 Jin Kousaka , Atsuko H. Iwane , Yuichi Togashi

Plants frequently contain numerous organs, organized in 3D branching systems defining the plant's architecture. Reconstructing the architecture of plants from unstructured observations is challenging because of self-occlusion and spatial…

计算机视觉与模式识别 · 计算机科学 2026-04-02 Samara Ghrer , Christophe Godin , Stefanie Wuhrer

Thin leaves, fine stems, self-occlusion, non-rigid and slowly changing structures make plants difficult for three-dimensional (3D) scanning and reconstruction -- two critical steps in automated visual phenotyping. Many current solutions…

计算机视觉与模式识别 · 计算机科学 2017-02-28 Chuong V Nguyen , Jurgen Fripp , David R Lovell , Robert Furbank , Peter Kuffner , Helen Daily , Xavier Sirault

Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…

We tackle the challenging problem of creating full and accurate three dimensional reconstructions of botanical trees with the topological and geometric accuracy required for subsequent physical simulation, e.g. in response to wind forces.…

计算机视觉与模式识别 · 计算机科学 2018-12-24 Ed Quigley , Winnie Lin , Yilin Zhu , Ronald Fedkiw

Plant phenotyping plays a pivotal role in understanding plant traits and their interactions with the environment, making it crucial for advancing precision agriculture and crop improvement. 3D reconstruction technologies have emerged as…

图像与视频处理 · 电气工程与系统科学 2025-05-05 Jiajia Li , Xinda Qi , Seyed Hamidreza Nabaei , Meiqi Liu , Dong Chen , Xin Zhang , Xunyuan Yin , Zhaojian Li

Biomaterials are becoming an essential tool in the study and application of stem cell research. Various types of biomaterials enable three-dimensional (3D) culture of stem cells, and, more recently, also enable high-resolution patterning…

组织与器官 · 定量生物学 2016-10-11 Richard J. McMurtrey

While high-resolution microscopic techniques are crucial for studying cellular structures in cell biology, obtaining such images from thick 3D engineered tissues remains challenging. In this review, we explore advancements in fluorescence…

组织与器官 · 定量生物学 2025-02-07 Yasaman Kargar Gaz Kooh , Nathaniel Huebsch

Structural reconstruction of plant roots from MRI is challenging, because of low resolution and low signal-to-noise ratio of the 3D measurements which may lead to disconnectivities and wrongly connected roots. We propose a two-stage…

计算机视觉与模式识别 · 计算机科学 2020-10-28 Jannis Horn , Yi Zhao , Nils Wandel , Magdalena Landl , Andrea Schnepf , Sven Behnke

Epithelial cells form diverse structures from squamous spherical organoids to densely packed pseudostratified tissues. Quantification of cellular properties in these contexts requires high-resolution deep imaging and computational…

定量方法 · 定量生物学 2025-02-03 G. Paci , P. Vicente-Munuera , I. Fernandez-Mosquera , A. Miranda , K. Lau , Q. Zhang , R. Barrientos , Y. Mao

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

图像与视频处理 · 电气工程与系统科学 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

图像与视频处理 · 电气工程与系统科学 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

Reconstruction based on the stereo camera has received considerable attention recently, but two particular challenges still remain. The first concerns the need to aggregate similar pixels in an effective approach, and the second is to…

计算机视觉与模式识别 · 计算机科学 2017-03-31 Lei Fan , Ziyu Pan , Long Chen , Kai Huang

The orientation of cell division is a major determinant of three-dimensional plant morphogenesis. Whether and how a simple division orientation rule explains the establishment of symmetric body plans is a fundamental question. Testing such…

细胞行为 · 定量生物学 2026-05-14 Naoya Kamamoto , Koichi Fujimoto

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…

流体动力学 · 物理学 2017-09-19 S. Quint , A. F. Christ , A. Guckenberger , S. Himbert , L. Kaestner , S. Gekle , C. Wagner

How morphogenesis depends on cell properties is an active direction of research. Here, we focus on mechanical models of growing plant tissues, where microscopic (sub)cellular structure is taken into account. In order to establish links…

偏微分方程分析 · 数学 2023-11-21 Arezki Boudaoud , Annamaria Kiss , Mariya Ptashnyk

How a single fertilized cell gives rise to a complex array of specialized cell types in development is a central question in biology. The cells grow, divide, and acquire differentiated characteristics through poorly understood molecular…

机器学习 · 计算机科学 2025-03-26 Da Kuang , Guanwen Qiu , Junhyong Kim

In many tissues, cell type varies over single-cell length-scales, creating detailed spatial heterogeneities fundamental to physiological function. To gain understanding of this relationship between tissue function and detailed structure,…

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