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DNA translocation through nanopores is one of the most promising strategies for the next-generation sequencing technologies. Most part of experimental and numerical works has focused on polymer translocation biased by electrophoresis, where…

软凝聚态物质 · 物理学 2018-02-07 Timothée Menais

The twisting deformation of mechanically stretched DNA molecules is studied by a coarse grained Hamiltonian model incorporating the fundamental interactions that stabilize the double helix and accounting for the radial and angular base pair…

软凝聚态物质 · 物理学 2017-12-15 Marco Zoli

In this paper, we have studied, within a discrete WLC model, the spatial constraints in magnetic tweezers used in single molecule experiments. Two elements are involved: first, the fixed plastic slab on which is stuck the initial strand,…

生物大分子 · 定量生物学 2009-11-13 C. Bouchiat

A DNA-protein complex modelled by a semiflexible chain and an attractive spherical core is studied in the situation when an external stretching force is acting on one end monomer of the chain while the other end monomer is kept fixed in…

软凝聚态物质 · 物理学 2009-11-10 Takahiro Sakaue , Hartmut Löwen

In this dissertation we analyzed the double-layer force interaction between two dielectric charged colloidal spheres of different radii immersed in a solution of ions in water and in thermal equilibrium. In the limit of low electrostatic…

软凝聚态物质 · 物理学 2016-03-01 Daniel Martínez Tibaduiza

Experimental variables of optical tweezers instrumentation that affect RNA folding/unfolding kinetics were investigated. A model RNA hairpin, P5ab, was attached to two micron-sized beads through hybrid RNA/DNA handles; one bead was trapped…

生物物理 · 物理学 2009-11-13 J. -D. Wen , M. Manosas , P. T. X. Li , S. B. Smith , C. Bustamante , F. Ritort , I. Tinoco

We demonstrate the realization of (laterally) optically bounded colloidal structures on a liquid-liquid interface of an emulsion droplet. We use DNA tethers to graft the particles to the droplet surface, effectively confining them to a…

软凝聚态物质 · 物理学 2017-03-27 Alessio Caciagli , Darshana Joshi , Jurij Kotar , Erika Eiser

The strength of the spin-orbit interaction relevant to transport in a low dimensional structure depends critically on the relative geometrical arrangement of current carrying orbitals. Recent tight-binding orbital models for spin transport…

介观与纳米尺度物理 · 物理学 2017-10-20 Solmar Varela , Vladimiro Mujica , Ernesto Medina

The hybridization and dehybridization of DNA subject to tension is relevant to fundamental genetic processes and to the design of DNA-based mechanobiology assays. While strong tension accelerates DNA melting and decelerates DNA annealing,…

生物物理 · 物理学 2022-04-20 Derek J. Hart , Jiyoun Jeong , James C. Gumbart , Harold D. Kim

The symmetries of the DNA double helix require a new term in its linear response to stress: the coupling between twist and stretch. Recent experiments with torsionally-constrained single molecules give the first direct measurement of this…

软凝聚态物质 · 物理学 2009-10-30 Philip Nelson

Dielectrophoretic trapping of six different DNA fragments, sizes varying from the 27 to 8416 bp, has been studied using confocal microscopy. The effect of the DNA length and the size of the constriction between nanoscale fingertip…

We investigate the effects of crowding on the conformations and assembly of confined, highly charged, and thick polyelectrolyte brushes in the osmotic regime. Particle tracking experiments on increasingly dense suspensions of colloids…

We discuss theoretically the force F between two colloidal particles, each of them carrying one single strand DNA. The two strands are complementary only on a finite sequence of consecutive base pairs. We define an adjustment length (a few…

生物物理 · 物理学 2015-06-26 Pierre-Gilles de Gennes

The effective interaction between two parallel strands of helical bio-molecules, such as deoxyribose nucleic acids (DNA), is calculated using computer simulations of the "primitive" model of electrolytes. In particular we study a simple…

生物物理 · 物理学 2009-10-31 E. Allahyarov , H. Loewen

DNA condensation by multivalent cations plays a crucial role in genome packaging in viruses and sperm heads, and has been extensively studied using single-molecule experimental methods. In those experiments, the values of the critical…

软凝聚态物质 · 物理学 2015-04-13 Ruggero Cortini , Bertrand R. Caré , Jean-Marc Victor , Maria Barbi

We use optical tweezers to directly measure the intermolecular forces acting on a single polymer imposed by surrounding entangled polymers (115 kbp DNA, 1 mg/ml). A tube-like confining field was measured in accord with the key assumption of…

软凝聚态物质 · 物理学 2009-11-13 Rae M. Robertson , Douglas E. Smith

We study the control parameters that govern the dynamics of in vitro DNA ejection in bacteriophage lambda. Past work has demonstrated that bacteriophage DNA is highly pressurized; this pressure has been hypothesized to help drive DNA…

生物大分子 · 定量生物学 2015-05-18 David Wu , David Van Valen , Qicong Hu , Rob Phillips

Dual trap optical tweezers are often used in high-resolution measurements in single-molecule biophysics. Such measurements can be hindered by the presence of extraneous noise sources, the most prominent of which is the coupling of…

生物物理 · 物理学 2013-07-03 Marco Ribezzi Crivellari , Felix Ritort

Several controversial issues concerning the packing of linear DNA in bacteriophages and globules are discussed. Exact relations for the osmotic pressure, capsid pressure and loading force are derived in terms of the hole size inside phages…

软凝聚态物质 · 物理学 2007-05-23 Theo Odijk

The scaling properties of DNA knots of different complexities were studied by atomic force microscope. Following two different protocols DNA knots are adsorbed onto a mica surface in regimes of (i) strong binding, that induces a kinetic…