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We explain and demonstrate a new method of force- and position-calibration for optical tweezers with back-focal-plane photo detection. The method combines power spectral measurements of thermal motion and the response to a sinusoidal motion…

In granular media, the presence of even small amounts of interparticle cohesion manifests as an increase in the bulk strength and stiffness, effects that are typically associated with an increase in the average number of constraints per…

软凝聚态物质 · 物理学 2026-03-10 Abrar Naseer , Karen E. Daniels , Tejas G. Murthy

When pulled along the axis, double-strand DNA undergoes a large conformational change and elongates roughly twice its initial contour length at a pulling force about 70 pN. The transition to this highly overstretched form of DNA is very…

软凝聚态物质 · 物理学 2008-09-11 Mogurampelly Santosh , Prabal K Maiti

DNA-spools, structures in which DNA is wrapped and helically coiled onto itself or onto a protein core are ubiquitous in nature. We develop a general theory describing the non-equilibrium behavior of DNA-spools under linear tension. Two…

软凝聚态物质 · 物理学 2009-11-10 I. M. Kulic , H. Schiessel

Double-stranded DNA `overstretches' at a pulling force of about 65 pN, increasing in length by a factor of 1.7. The nature of the overstretched state is unknown, despite its considerable importance for DNA's biological function and…

统计力学 · 物理学 2009-11-13 Stephen Whitelam , Sander Pronk , Phillip L. Geissler

Acoustic tweezers can manipulate microscopic objects and cells independently of the optical, magnetic and electrical properties of the objects or their medium. However, because ultrasonic waves are attenuated within few millimeters,…

流体动力学 · 物理学 2022-03-29 Qing Wang , Shuhan Chen , Jia Zhou , Antoine Riaud

Many-body hydrodynamic interactions (HIs) play an important role in the dynamics of fluid suspensions. While many-body HIs have been studied extensively using particle simulations, there is a dearth of experimental frameworks with which to…

软凝聚态物质 · 物理学 2025-02-17 Dae Yeon Kim , Sachit G. Nagella , Kyu Hwan Choi , Sho C. Takatori

We present a statistical mechanical model of aggregation in colloidal systems with DNA mediated interactions. We obtain a general result for the two-particle binding energy in terms of the hybridization free energy $\Delta G$ of DNA and two…

统计力学 · 物理学 2009-11-11 Nicholas A. Licata , Alexei V. Tkachenko

We investigate the thermodynamics and kinetics of DNA hairpins that fold/unfold under the action of applied mechanical force. We introduce the concept of the molecular free energy landscape and derive simplified expressions for the force…

统计力学 · 物理学 2009-09-30 Alessandro Mossa , Maria Manosas , Nuria Forns , Josep Maria Huguet , Felix Ritort

We propose a new method for electrophoretic separation of DNA in which adsorbed polymers are driven over a disordered two-dimensional substrate which contains attractive sites for the polymers. Using simulations of a model for long polymer…

软凝聚态物质 · 物理学 2008-03-31 C. J. Olson Reichhardt , C. Reichhardt

Generation of mechanical force regulated by external electric field is studied both theoretically and by molecular dynamics (MD) simulations. The force arises in deformable bodies linked to the free end of a grafted polyelectrolyte chain…

统计力学 · 物理学 2014-11-11 Christian Seidel , Yury A. Budkov , Nikolay V. Brilliantov

We consider an elastic rod model for twisted DNA in the plectonemic regime. The molecule is treated as an impenetrable tube with an effective, adjustable radius. The model is solved analytically and we derive formulas for the contact…

生物大分子 · 定量生物学 2009-11-13 N. Clauvelin , B. Audoly , S. Neukirch

Solid-state nanopores are single molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage and salt dependence of the frequency of…

生物物理 · 物理学 2016-03-23 Nicholas A. W. Bell , Murugappan Muthukumar , Ulrich F. Keyser

Screening of a surface charge by electrolyte and the resulting interaction energy between charged objects is of fundamental importance in scenarios from bio-molecular interactions to energy storage. The conventional wisdom is that the…

化学物理 · 物理学 2017-01-30 Alpha A. Lee , Carla Perez-Martinez , Alexander M. Smith , Susan Perkin

This paper aims at a comprehensive understanding on the novel elastic property of double-stranded DNA (dsDNA) discovered very recently through single-molecule manipulation techniques. A general elastic model for double-stranded biopolymers…

软凝聚态物质 · 物理学 2009-10-31 Haijun Zhou , Yang Zhang , Zhong-can Ou-Yang

Recent theoretical predictions on DNA mechanical separation induced by pulling forces are numerically tested within a model in which self-avoidance for DNA strands is fully taken into account. DNA strands are described by interacting pairs…

The behavior of polyelectrolytes between charged surfaces immersed in semi-dilute solutions is investigated theoretically. A continuum mean field approach is used for calculating numerically concentration profiles between two electrodes…

软凝聚态物质 · 物理学 2007-05-23 I. Borukhov , D. Andelman , H. Orland

The mechanical separation of the double helical DNA structure induced by forces pulling apart the two DNA strands (``unzipping'') has been the subject of recent experiments. Analytical results are obtained within various models of…

统计力学 · 物理学 2009-11-07 D. Marenduzzo , A. Trovato , A. Maritan

Non-invasive optical manipulation of particles has emerged as a powerful and versatile tool for biological study and nanotechnology. In particular, trapping and rotation of cells, cell nuclei and sub-micron particles enables unique…

The distance-resolved effective interaction potential between two parallel DNA molecules is calculated by computer simulations with explicit tetravalent counterions and monovalent salt. Adding counterions first yields an attractive minimum…

软凝聚态物质 · 物理学 2009-11-10 E. Allahyarov , H. Löwen , G. Gompper
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