定量方法
Objective: Murmurs are abnormal heart sounds, identified by experts through cardiac auscultation. The murmur grade, a quantitative measure of the murmur intensity, is strongly correlated with the patient's clinical condition. This work aims…
Any reliable biomarker has to be specific, generalizable, and reproducible across individuals and contexts. The exact values of such a biomarker must represent similar health states in different individuals and at different times within the…
CRISPR-Cas13 is a system that utilizes single stranded RNAs for RNA editing. Prediction of on-target and off-target effects for the CRISPR-Cas13d dependency enables us to design specific single guide RNAs (sgRNAs) that help locate the…
Patient-derived extracellular vesicles (EVs) that contains a complex biological cargo is a valuable source of liquid biopsy diagnostics to aid in early detection, cancer screening, and precision nanotherapeutics. In this study, we predicted…
Protein design is a technique to engineer proteins by modifying their sequence to obtain novel functionalities. In this method, amino acids in the sequence are permutated to find the low energy states satisfying the configuration. However,…
Cell fate decisions emerge as a consequence of a complex set of gene regulatory networks. Models of these networks are known to have more parameters than data can determine. Recent work, inspired by Waddington's metaphor of a landscape, has…
Fed-batch culture is an established operation mode for the production of biologics using mammalian cell cultures. Quantitative modeling integrates both kinetics for some key reaction steps and optimization-driven metabolic flux allocation,…
In the field of antibody engineering, an essential task is to design a novel antibody whose paratopes bind to a specific antigen with correct epitopes. Understanding antibody structure and its paratope can facilitate a mechanistic…
Culling newly hatched male chicks in industrial hatcheries poses a serious ethical problem. Both laying and broiler breeders need males, but it is a problem because they are produced more than needed. Being able to determine the sex of…
The shape of erythrocytes or red blood cells is altered in several pathological conditions. Therefore, identifying and quantifying different erythrocyte shapes can help diagnose various diseases and assist in designing a treatment strategy.…
Motivation: Machine learning methods can be used to support scientific discovery in healthcare-related research fields. However, these methods can only be reliably used if they can be trained on high-quality and curated datasets. Currently,…
Motivation: The size of available omics datasets is steadily increasing with technological advancement in recent years. While this increase in sample size can be used to improve the performance of relevant prediction tasks in healthcare,…
Structural alterations of the midsagittal corpus callosum (midCC) have been associated with a wide range of brain disorders. The midCC is visible on most MRI contrasts and in many acquisitions with a limited field-of-view. Here, we present…
In this paper, we introduce PathRTM, a novel deep neural network detector based on RTMDet, for automated KI-67 proliferation and tumor-infiltrated lymphocyte estimation. KI-67 proliferation and tumor-infiltrated lymphocyte estimation play a…
Approximate Bayesian Computation (ABC) is a widely applicable and popular approach to estimating unknown parameters of mechanistic models. As ABC analyses are computationally expensive, parallelization on high-performance infrastructure is…
Allostery plays a crucial role in regulating protein activity, making it a highly sought-after target in drug development. One of the major challenges in allosteric drug research is the identification of allosteric sites. In recent years,…
The alignment of biological sequences such as DNA, RNA, and proteins, is one of the basic tools that allow to detect evolutionary patterns, as well as functional/structural characterizations between homologous sequences in different…
Recent advances in immunomics have shown that T-cell receptor (TCR) signatures can accurately predict active or recent infection by leveraging the high specificity of TCR binding to disease antigens. However, the extreme diversity of the…
Within the soundscapes of open-plan offices, irrelevant speech has consistently been reported as the most distracting, and causing performance decrements for workers. Notwithstanding this generalization, the 'babble' created by multiple…
The changes in stromal collagen play a crucial role during the pathogenesis and progression of pancreatic intraepithelial neoplasm (PanIN) to pancreatic ductal adenocarcinoma (PDAC) while misdiagnosis of PanIN is common because of the…