X射线揭示拥挤蛋白溶液中异常分子扩散及笼效应
软凝聚态物质
2025-12-04 v2 化学物理
摘要
了解细胞内蛋白质运动对于预测反应速率和细胞质中的macromolecular运输至关重要。一个关键问题是拥挤环境如何通过水动力学效应和在分子尺度上的直接相互作用影响蛋白质动力学。本文利用欧洲X射线自由电子激光器(EuXFEL)上的百兆赫X射线光相关谱(XPCS)技术，研究了铁蛋白在微秒时间尺度上的扩散行为。我们的结果揭示了异常扩散现象，这一行为表现为在高浓度下，强度自相关函数呈非指数衰减，与笼式捕获在短时间和长时间蛋白质扩散 regime 之间存在相吻合之况相符。通过纳入与蛋白质直接相互作用相关的比例因子，运用水动力相互作用群体的δγ理论对实验数据进行建模，成功复现了实验结果。这一发现为理解拥挤蛋白溶液中复杂的分子运动提供了新视角，对于优化以蛋白质扩散为限的铁蛋白制剂递送系统具有潜在应用价值。
引用
@article{arxiv.2410.08873,
title = {Coherent X-rays reveal anomalous molecular diffusion and cage effects in crowded protein solutions},
author = {Anita Girelli and Maddalena Bin and Mariia Filianina and Michelle Dargasz and Nimmi Das Anthuparambil and Johannes Möller and Alexey Zozulya and Iason Andronis and Sonja Timmermann and Sharon Berkowicz and Sebastian Retzbach and Mario Reiser and Agha Mohammad Raza and Marvin Kowalski and Mohammad Sayed Akhundzadeh and Jenny Schrage and Chang Hee Woo and Maximilian D. Senft and Lara Franziska Reichart and Aliaksandr Leonau and Prince Prabhu Rajaiah and William Chèvremont and Tilo Seydel and Jörg Hallmann and Angel Rodriguez-Fernandez and Jan-Etienne Pudell and Felix Brausse and Ulrike Boesenberg and James Wrigley and Mohamed Youssef and Wei Lu and Wonhyuk Jo and Roman Shayduk and Trey Guest and Anders Madsen and Felix Lehmkühler and Michael Paulus and Fajun Zhang and Frank Schreiber and Christian Gutt and Fivos Perakis},
journal= {arXiv preprint arXiv:2410.08873},
year = {2025}
}
备注
This version of the article has been accepted for publication after peer review, but is not the Version of Record and does not reflect post-acceptance improvements or any corrections. The Version of Record is available online at: https://doi.org/10.1038/s41467-025-66972-6