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Mid-infrared photothermal (MIP) microscopy has been a promising label-free chemical imaging technique for functional characterization of specimens owing to its enhanced spatial resolution and high specificity. Recently developed wide-field…

Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The…

Mid-infrared photo-induced force microscopy (IR-PiFM/PiF-IR) enables high-resolution chemical imaging of surfaces with lateral resolution less than 5 nm. Here are some answers to questions about the physical background, practical handling…

Materials Science · Physics 2026-05-05 Daniela Täuber

Magnetic resonance microimaging (MR mu I) is an outstanding technique for studying water transfers in millimetric bio-based materials in a non-destructive and non-invasive manner. However, depending on the composition of the material,…

Signal Processing · Electrical Eng. & Systems 2024-09-04 Sílvia Mas Garcia , Jean-Michel Roger , Ruzica Ferbus , Denis Lourdin , Corinne C. Rondeau-Mouro

This paper presents for the first time an innovative instrument called an inverted scanning microwave microscope (iSMM), which is capable of noninvasive and label-free imaging and characterization of intracellular structures of a live cell…

We present a novel method for real-time monitoring and kinetic analysis of fluorescence in situ hybridization (FISH). We implement the method using a vertical microfluidic probe containing a microstructure designed for rapid switching…

Biomolecules · Quantitative Biology 2018-10-04 Nadya Ostromohov , Deborah Huber , Moran Bercovici , Govind V. Kaigala

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Multidimensional scaling (MDS) is a dimensionality reduction technique for microbial ecology data analysis that represents the multivariate structure while preserving pairwise distances between samples. While its improvement has enhanced…

Fish Feeding Intensity Assessment (FFIA) is crucial in industrial aquaculture management. Recent multi-modal approaches have shown promise in improving FFIA robustness and efficiency. However, these methods face significant challenges when…

Machine Learning · Computer Science 2025-04-22 Meng Cui , Xianghu Yue , Xinyuan Qian , Jinzheng Zhao , Haohe Liu , Xubo Liu , Daoliang Li , Wenwu Wang

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor…

Image and Video Processing · Electrical Eng. & Systems 2020-10-13 Kyrollos Yanny , Nick Antipa , William Liberti , Sam Dehaeck , Kristina Monakhova , Fanglin Linda Liu , Konlin Shen , Ren Ng , Laura Waller

Fourier Transform Interferometry (FTI) is an appealing Hyperspectral (HS) imaging modality for many applications demanding high spectral resolution, e.g., in fluorescence microscopy. However, the effective resolution of FTI is limited by…

Information Theory · Computer Science 2019-01-31 A. Moshtaghpour , L. Jacques , V. Cambareri , P. Antoine , M. Roblin

Microwave impedance microscopy (MIM) is an emerging scanning probe technique for nanoscale complex permittivity mapping and has made significant impacts in diverse fields from semiconductors to quantum materials. To date, the most…

Mesoscale and Nanoscale Physics · Physics 2024-06-14 Jun-Yi Shan , Nathaniel Morrison , Su-Di Chen , Feng Wang , Eric Y. Ma

Spectral molecular imaging is a new imaging technique able to discriminate and quantify different components of tissue simultaneously at high spatial and high energy resolution. Our MARS scanner is an x-ray based small animal CT system…

Microscope mode imaging for secondary ion mass spectrometry is a technique with the promise of simultaneous high spatial resolution and high speed imaging of biomolecules from complex surfaces. Technological developments such as new…

Instrumentation and Detectors · Physics 2013-09-05 András Kiss , Donald F. Smith , Julia H. Jungmann , Ron M. A. Heeren

Omnidirectional multi-view stereo (MVS) vision is attractive for its ultra-wide field-of-view (FoV), enabling machines to perceive 360{\deg} 3D surroundings. However, the existing solutions require expensive dense depth labels for…

Computer Vision and Pattern Recognition · Computer Science 2023-02-23 Zisong Chen , Chunyu Lin , Lang Nie , Kang Liao , Yao Zhao

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

We present a fast and accurate analytical method for fluorescence lifetime imaging microscopy (FLIM) using the extreme learning machine (ELM). We used extensive metrics to evaluate ELM and existing algorithms. First, we compared these…

Biological Physics · Physics 2022-03-28 Zhenya Zang , Dong Xiao , Quan Wang , Zinuo Li , Wujun Xie , Yu Chen , David Day Uei Li

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

We use both FEM (finite element method) and FDTD (finite difference time domain method) to simulate the field distribution in Maxwell's fish eye lens with one or more passive drains around the image point. We use the same Maxwell's fish eye…

Optics · Physics 2010-11-29 Fei Sun , Xiaochen Ge , Sailing He

Optical tweezers are a technique in which microscopic-sized particles, including living cells and bacteria, can be non-intrusively trapped with high accuracy solely using focused light. The technique has therefore become a powerful tool in…

Biological Physics · Physics 2015-03-04 Magnus Andersson